Characterization of extended-spectrum β-lactamases (ESBLs)-producing Salmonella in retail raw chicken carcasses

Qiao, Jing; Zhang, Qiang; Alali, Walid Q.; Wang, Jiawei; Lingyuan, Meng; Xiao, Yingping; Yang, Hua; Chen, Sheng; Cui, Shenghui; Yang, Baowei

doi:10.1016/j.ijfoodmicro.2017.02.016

Cited by 55 publications

(42 citation statements)

References 59 publications

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“…In the present study, the most commonly detected ESBLs encoding genes were OXAs (42.9%), followed by CTX-Ms (23.8%). This was similar to the results of previous studies in which CTX-M- types were the main family of ESBLs ( Nguyen et al, 2016 ; Qiao et al, 2017 ). Furthermore, reports indicated that CTX-Ms have been disseminating rapidly among populations of gram-negative bacteria in clinical settings in recent years ( Shahid et al, 2009 ).…”

Section: Discussionsupporting

confidence: 92%

“…ESBLs encoding genes detected included bla TEM , bla CTX-M , bla CMY , bla OXA , bla PSE , and bla SHV . The primers for the ESBLs encoding gene and the reaction conditions were as previously described ( Li et al, 2013 ; Nguyen et al, 2016 ; Qiao et al, 2017 ). The PCR products were stained with ethidium bromide, visualized under UV light after gel electrophoresis using 1% agarose, purified, and sent to Sangon Biotech Co., Ltd. (Shanghai, China) for sequencing.…”

Section: Methodsmentioning

confidence: 99%

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Highly Prevalent Multidrug-Resistant Salmonella From Chicken and Pork Meat at Retail Markets in Guangdong, China

et al. 2018

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This study aimed to investigate the prevalence, serotype distribution, and antibiotic resistance, and to characterize the extended spectrum β-lactamases (ESBLs) producing Salmonella isolates from chicken and pork meats from retail markets in Guangdong province, China. A total of 903 retail meat samples (475 chicken and 428 pork meats) were obtained from six cities (Guangzhou, Shenzhen, Heyuan, Shaoguan, Foshan, and Yunfu) of Guangdong province between May 2016 and April 2017. High levels of Salmonella contamination were detected in chicken (302/475, 63.6%) and pork (313/428, 73.1%). Thirty-eight serotypes were identified in 615 detected Salmonella, and the serotypes varied greatly between chicken and pork samples. Agona (55/302, 18.2%), Corvallis (45/302, 14.9%), Kentucky (38/302, 12.6%), Mbandaka (32/302, 10.6%) was the dominant serotypes in chicken samples. However, Typhimurium (78/313, 24.9%), Rissen (67/313, 24.1%), Derby (66/313, 21.1%), and London (48, 15.3%) were the most common in pork samples. High rates of antibiotic resistance were found to sulfisoxazole (468/615, 76.1%), tetracycline (463/615, 75.3%), ampicillin (295/615, 48.0%), and ofloxacin (275/615, 44.7%). Notably, antimicrobial susceptibility tests identified resistance to polymyxin B (12/615, 2.0%) and imipenem (3/615, 0.5%). Multidrug-resistance (MDR) was detected in Salmonella isolated from chicken (245/302, 81.1%) and pork (229/313, 73.2%). The resistance rate of different Salmonella serotypes varied widely. Especially, isolates such as Typhimurium, Agona, Corvallis and Kentucky exhibited highly resistance to antibiotics. The MDR rate of Salmonella isolates from chicken was significantly higher than that from pork isolates (P < 0.05). Twenty-one Salmonella isolates were identified as ESBLs-producing, covering six Salmonella serotypes and displaying different pulse field gel electrophoresis (PFGE) genotypes. BlaOXA-1 was the dominant ESBLs gene (9/21, 42.9%), followed by blaCTX-M-55 (5/21, 23.8%). This study indicated that Salmonella was widespread in chicken and pork from retail markets in Guangdong province and the isolates showed high multidrug-resistance, especially the known multidrug-resistant Salmonella serotypes. Therefore, it is important to focus on Salmonella serotypes and strengthen the long-term monitoring of MDR Salmonella serotypes in animal-derived foods.

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Section: Discussionsupporting

confidence: 92%

Section: Methodsmentioning

confidence: 99%

Highly Prevalent Multidrug-Resistant Salmonella From Chicken and Pork Meat at Retail Markets in Guangdong, China

et al. 2018

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“…The β-lactamase encoding gene bla TEM conferring resistance to penicillins and first-generation cephalosporins was detected in 73.3% of S. enterica serovars and in 76.9% of ampicillin-resistant isolates which is significantly high in comparison to 57.3% ( bla TEM-1 ) in S. enterica isolated from retail chickens in China [ 47 ]. Another β-lactamase encoding gene bla CMY-2 , an AmpC beta-lactamase gene that confers resistance to a wide variety of β-lactam antibiotics detected in 13.3% of isolates.…”

Section: Discussionmentioning

confidence: 99%

Occurrence of Salmonella enterica and Escherichia coli in raw chicken and beef meat in northern Egypt and dissemination of their antibiotic resistance markers

et al. 2017

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BackgroundThe global incidence of foodborne infections and antibiotic resistance is recently increased and considered of public health concern. Currently, scarcely information is available on foodborne infections and ESBL associated with poultry and beef meat in Egypt.MethodsIn total, 180 chicken and beef meat samples as well as internal organs were collected from different districts in northern Egypt. The samples were investigated for the prevalence and antibiotic resistance of Salmonella enterica serovars and Escherichia coli. All isolates were investigated for harbouring class 1 and class 2 integrons.ResultsOut of 180 investigated samples 15 S. enterica (8.3%) and 21 E. coli (11.7%) were isolated and identified. S. enterica isolates were typed as 9 S. Typhimurium (60.0%), 3 S. Paratyphi A (20.0%), 2 S. Enteritidis (13.3%) and 1 S. Kentucky (6.7%). Twenty-one E. coli isolates were serotyped into O1, O18, O20, O78, O103, O119, O126, O145, O146 and O158. The phenotypic antibiotic resistance profiles of S. enterica serovars to ampicillin, cefotaxime, cefpodoxime, trimethoprim/sulphamethoxazole and tetracycline were 86.7, 80.0, 60.0, 53.3 and 40.0%, respectively. Isolated E. coli were resistant to tetracycline (80.9%), ampicillin (71.4%), streptomycin, trimethoprim/sulphamethoxazole (61.9% for each) and cefotaxime (33.3%). The dissemination of genes coding for ESBL and AmpC β-lactamase in S. enterica isolates included bla CTX-M (73.3%), bla TEM (73.3%) and bla CMY (13.3%). In E. coli isolates bla TEM, bla CTX-M and bla OXA were identified in 52.4, 42.9 and 14.3%, respectively. The plasmid-mediated quinolone resistance genes identified in S. enterica were qnrA (33.3%), qnrB (20.0%) and qnrS (6.7%) while qnrA and qnrB were detected in 33.3% of E. coli isolates. Class 1 integron was detected in 13.3% of S. enterica and in 14.3% of E. coli isolates. Class 2 integron as well as the colistin resistance gene mcr-1 was not found in any of E. coli or S. enterica isolates.ConclusionsThis study showed high prevalence of S. enterica and E. coli as foodborne pathogens in raw chicken and beef meat in Nile Delta, Egypt. The emergence of antimicrobial resistance in S. enterica and E. coli isolates is of public health concern in Egypt. Molecular biological investigation elucidated the presence of genes associated with antibiotic resistance as well as class 1 integron in S. enterica and E. coli.

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“…2), mostly in studies performed after 2010 (with few exceptions [21,38,43,52,54,61]). Carbapenemaseencoding genes in CRE isolates from livestock or (sea)food items were first described in China (2007e2012) [22] and Germany (2011) [38e42]. The following carbapenemase genes were [19,35,36,48e50], and imipenem-carbapenemase (IMP-1, IMP-2, IMP-27) ( (Table 1).…”

Section: Occurrence Of Cre In Livestock and (Sea)foodmentioning

confidence: 99%

Carbapenem-resistant Enterobacteriaceae in wildlife, food-producing, and companion animals: a systematic review

Köck

Daniels-Haardt²,

Becker

et al. 2018

Clinical Microbiology and Infection

252

167

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Characterization of extended-spectrum β-lactamases (ESBLs)-producing Salmonella in retail raw chicken carcasses

Cited by 55 publications

References 59 publications

Highly Prevalent Multidrug-Resistant Salmonella From Chicken and Pork Meat at Retail Markets in Guangdong, China

Highly Prevalent Multidrug-Resistant Salmonella From Chicken and Pork Meat at Retail Markets in Guangdong, China

Occurrence of Salmonella enterica and Escherichia coli in raw chicken and beef meat in northern Egypt and dissemination of their antibiotic resistance markers

Carbapenem-resistant Enterobacteriaceae in wildlife, food-producing, and companion animals: a systematic review

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