Characterization of Extended-Spectrum β-Lactamase-Producing
            <i>Escherichia coli</i>
            Isolates That Cause Diarrhea in Sheep in Northwest China

Zhao, Xueliang; Zhao, Haoyu; Zhou, Zilian; Miao, Yongqiang; Li, Ruichao; Yang, Baowei; Cao, Chenyang; Xiao, Sa; Wang, Xinglong; Liu, Haijin; Wang, Juan; Yang, Zhicong

doi:10.1128/spectrum.01595-22

Cited by 13 publications

(13 citation statements)

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“…Consistent with findings in previous studies, our isolates demonstrate a high diversity of serotypes and STs. Notably, most of the serotypes were pathogenic for animals and even humans in our previous study (Zhao et al., 2022). ST types of ESBLs‐producing E. coli strains were complex and exhibit a high genetic diversity.…”

Section: Discussionmentioning

confidence: 64%

“…The number of virulence genes of different functional categories in each strain was plotted and sorted according to the phylogenetic position on the tree. Note : ‘–’: the new sequence type; “/”: the novel serotype; a : data are from a previous study (Zhao et al., 2022). VFDB (no.…”

Section: Resultsmentioning

confidence: 99%

“…The dendrogram of PFGE patterns was constructed using BioNumerics 7.6 with unweighted pair group method using average linkages (UPGMA) clustering. Plasmid replicons were analyzed using Plasmid‐Finder v.2.1 with 60% minimum coverage and 95% minimum identity (Plasmid replicons are from a previous study (Zhao et al, 2022)).…”

Section: Resultsmentioning

confidence: 99%

“…Virulence genes in the complete genome were predicted using the virulence factor database (VFDB) in VirulenceFinder 2.0 (https://cge.food.dtu.dk/services/VirulenceFinder/) and VFDB (http://www.mgc.ac.cn/VFs/main.htm). The serotypes and MLST of all ESBLs‐producing E. coli were predicted using SerotypeFinder and MLST website, respectively (data are from a previous study) (Zhao et al., 2022). The PathogenFinder (https://cge.food.dtu.dk/services/PathogenFinder/) was used to assess pathogenicity towards humans in E. coli genomes (Cosentino et al., 2013).…”

Section: Methodsmentioning

confidence: 99%

“…A collection of ESBL‐producing E. coli ( n = 67) from cloacal swabbing of sheep suffering from severe watery diarrhoea were isolated and identified from Shaanxi, Ningxia, Inner Mongolia and Qinghai Provinces. The detailed information is described in previous studies (Zhao et al., 2021, 2022). Pathogenic E .…”

Section: Methodsmentioning

confidence: 99%

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ESBLs‐producing Escherichia coli from sheep‐origin: Genome‐wide virulence genes identification and in vivo virulence assessment in mice and Galleria mellonella

Zhao¹,

Miao²,

Adam³

et al. 2022

Transbounding Emerging Dis

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The worldwide spread of pathogenic Escherichia coli, together with the multidrug resistant linked with extended‐spectrum β‐lactamases (blaCTX‐M, blaTEM and blaOXA), not only affect the health of animals and humans but also bring huge economic losses to animal husbandry. Despite the high levels of virulence present in many extended‐spectrum beta‐lactamases (ESBLs)‐producing E. coli isolates, however, few studies have comprehensively assessed the pathogenicity of ESBLs‐producing E. coli isolates. Thus, the aim of the present study was to investigate the presence of virulence genes in third‐generation cephalosporin–resistant E. coli and to assess their pathogenicity and zoonotic potential. Previously, we identified 67 ESBLs‐producing E. coli strains from sheep anal swabs in northwest China. In this study, we genotypically and phenotypically characterized isolates of E. coli that produce ESBLs. According to the VirulenceFinder and virulence factors database, all ESBLs‐producing E. coli strains harboured a wide range of virulence genes. The ColV plasmid‐related genes (hlyF, ompT, iss, iutA and cvaC) were present in 52 (77.6%) ESBLs‐producing E. coli isolates. Surprisingly, quite a number of extraintestinal pathogenic E. coli virulence‐related genes were detected in 62 (92.5%) of 67 isolates. A total of 33 serotypes and 37 sequence types (STs) were found in 67 ESBLs‐producing isolates. ST10 is the most prevalent ST, which is represented by five strains. The cluster analysis showed that CC10 and CC23 were the common clonal complexes (CCs). Predominant serotypes were O8 (10%) and O9 (9%) followed by 6% each of O89, O101 and O185. Most sheep‐origin ESBLs‐producing E. coli held the highly pathogenic to human and displayed moderate‐to‐vigorous‐intensity motor capacity. The ESBLs‐producing E. coli isolates with numerous virulence‐related genes were able to cause multiple infectious diseases in animal models (mice, neonatal rats and Galleria mellonella). To our knowledge, this study represents an important first step for a comprehensive characterization of pathogenicity and zoonotic potential of sheep‐origin ESBLs‐producing E. coli isolates. These findings may be of significant value for the identification of pathogenicity and zoonotic potential risks associated with sheep‐origin ESBLs‐producing E. coli.

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Section: Discussionmentioning

confidence: 64%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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ESBLs‐producing Escherichia coli from sheep‐origin: Genome‐wide virulence genes identification and in vivo virulence assessment in mice and Galleria mellonella

Zhao¹,

Miao²,

Adam³

et al. 2022

Transbounding Emerging Dis

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Genomic diversity, antibiotic resistance, and virulence in South African Enterococcus faecalis and Enterococcus lactis isolates

Olanrewaju,

Molale-Tom,

Bezuidenhout

2024

World J Microbiol Biotechnol

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This study presents the empirical findings of an in-depth genomic analysis of Enterococcus faecalis and Enterococcus lactis isolates from South Africa. It offers valuable insights into their genetic characteristics and their significant implications for public health. The study uncovers nuanced variations in the gene content of these isolates, despite their similar GC contents, providing a comprehensive view of the evolutionary diversity within the species. Genomic islands are identified, particularly in E. faecalis, emphasizing its propensity for horizontal gene transfer and genetic diversity, especially in terms of antibiotic resistance genes. Pangenome analysis reveals the existence of a core genome, accounting for a modest proportion of the total genes, with 2157 core genes, 1164 shell genes, and 4638 cloud genes out of 7959 genes in 52 South African E. faecalis genomes (2 from this study, 49 south Africa genomes downloaded from NCBI, and E. faecalis reference genome). Detecting large-scale genomic rearrangements, including chromosomal inversions, underscores the dynamic nature of bacterial genomes and their role in generating genetic diversity. The study uncovers an array of antibiotic resistance genes, with trimethoprim, tetracycline, glycopeptide, and multidrug resistance genes prevalent, raising concerns about the effectiveness of antibiotic treatment. Virulence gene profiling unveils a diverse repertoire of factors contributing to pathogenicity, encompassing adhesion, biofilm formation, stress resistance, and tissue damage. These empirical findings provide indispensable insights into these bacteria’s genomic dynamics, antibiotic resistance mechanisms, and virulence potential, underlining the pressing need to address antibiotic resistance and implement robust control measures.

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Current epidemiologic features and health dynamics of ESBL-producing Escherichia coli in China

Salman,

Umar,

Xiao

2024

Biosafety and Health

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Characterization of Extended-Spectrum β-Lactamase-Producing Escherichia coli Isolates That Cause Diarrhea in Sheep in Northwest China

Abstract: Antimicrobial resistance (AMR), especially the simultaneous resistance to several antibiotics (multidrug resistance [MDR]), is one of the greatest threats to global public health in the 21st century. Among animals, chickens, pigs, and cattle are reservoirs of these pathogens worldwide.

Cited by 13 publications

References 55 publications

ESBLs‐producing Escherichia coli from sheep‐origin: Genome‐wide virulence genes identification and in vivo virulence assessment in mice and Galleria mellonella

ESBLs‐producing Escherichia coli from sheep‐origin: Genome‐wide virulence genes identification and in vivo virulence assessment in mice and Galleria mellonella

Genomic diversity, antibiotic resistance, and virulence in South African Enterococcus faecalis and Enterococcus lactis isolates

Current epidemiologic features and health dynamics of ESBL-producing Escherichia coli in China

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