Characterization of Extended-Spectrum β-Lactamase–Producing Escherichia coli Isolates from Pigs and Farm Workers

Zhang, Hongna; Zhai, Zhenzhen; Li, Qing; Liu, Linghong; Guo, Shuyuan; Li, Qimeng; Yang, Lingling; Ye, Chaoqun; Chang, Wei-Shan; Zhai, Jing

doi:10.4315/0362-028x.jfp-16-093

Cited by 16 publications

(14 citation statements)

References 26 publications

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“…This prevalence is much higher than in other European countries such as the Netherlands, Germany and Denmark, in which the carriage rates of farm workers have been similar to the general population (2.5 to 13%) [36][37][38][39]. Rates similar to ours have been reported in China (20%) where colonization with ESBL-Enterobacteriaceae in healthy non-animal exposed humans was also very high, reaching up to 70% and carriage rate in pigs is reported above 60% [4,40]. In contrast to farmers, the phenotypic resistance to 3rd generation cephalosporins isolated from pigs in Estonia has been reported as low, i.e., 2.5% and 3.3% in healthy and 4.2% and 7.7% in diseased animals, for cefotaxime and ceftazidime, respectively [41].…”

Section: Discussionsupporting

confidence: 82%

Characteristics of Extended-Spectrum Beta-Lactamase-Producing Enterobacteriaceae and Contact to Animals in Estonia

et al. 2020

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We have attempted to define the prevalence and risk factors of extended-spectrum beta-lactamase-producing Enterobacteriaceae (ESBL-Enterobacteriaceae) carriage, and to characterize antimicrobial susceptibility, beta-lactamase genes, and major types of isolated strains in volunteers, with a specific focus on humans in contact with animals. Samples were collected from 207 volunteers (veterinarians, pig farmers, dog owners, etc.) and cultured on selective agar. Clonal relationships of the isolated ESBL-Enterobacteriaceae were determined by whole genome sequencing and multi-locus sequence typing. Beta-lactamases were detected using a homology search. Subjects filled in questionnaires analyzed by univariate and multiple logistic regression. Colonization with ESBL-Enterobacteriaceae was found in fecal samples of 14 individuals (6.8%; 95%CI: 3.75–11.09%). In multiple regression analysis, working as a pig farmer was a significant risk factor for ESBL-Enterobacteriaceae carriage (OR 4.8; 95%CI 1.2–19.1). The only species isolated was Escherichia coli that distributed into 11 sequence types. All ESBL-Enterobacteriaceae isolates were of CTX-M genotype, with the blaCTX-M-1 being the most prevalent and more common in pig farmers than in other groups. Despite the generally low prevalence of ESBL-Enterobacteriaceae in Estonia, the pig farmers may still pose a threat to transfer resistant microorganisms. The clinical relevance of predominant blaCTX-M-1 carrying E. coli is still unclear and needs further studies.

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Section: Discussionsupporting

confidence: 82%

Characteristics of Extended-Spectrum Beta-Lactamase-Producing Enterobacteriaceae and Contact to Animals in Estonia

et al. 2020

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“…bla CTX-M-14 is also frequently detected in farm animals, including pigs, and in pets in China. Although bla CTX-M-65 does occur in both humans and pets, it appears to be more common in pigs and broiler chickens (17)(18)(19)(20). The other bla CTX-M genes identified in this study (Table 1) are also frequently detected in humans and animals, especially bla CTX-M-55 among clinical isolates.…”

mentioning

confidence: 61%

Occurrence of bla _KPC-2 , bla _CTX-M , and mcr-1 in Enterobacteriaceae from Well Water in Rural China

Sun

Nilsson

et al. 2017

Antimicrob Agents Chemother

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We report on the coexistence of and in multidrug-resistant, extended-spectrum β-lactamase-producing belonging to the sequence type 10 complex isolated from well water in rural China. with was also detected in well water from the same area. This study shows that genes coding for resistance to last-resort antibiotics are present in wells in rural China, indicating a potential source of antibiotic resistance.

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“…Of note, ESBL-producing E. coli isolates were resistant to ampicillin and cefotaxime. The resistance to these antibiotics may be largely linked with the frequently use of ampicillin and cefotaxime in local human and animal clinical practices [43]. Our study provides evidence that poultry farms and retail chicken should be reflected as an important reservoir of ESBL-producing E. coli.…”

Section: Mics For Esbl-producing Escherichia Colimentioning

confidence: 79%

Multiple Antimicrobial Resistance of Extended Spectrum Beta-Lactamase-Producing Escherichia coli from Small-Scaled Poultry Farms and Retail Chicken

Kilonzo‐Nthenge¹,

Nahashon²,

Liu³

et al. 2018

JFSE

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Antibiotics used for agricultural purpose has contributed to the increased prevalence of antibiotic-resistant bacteria. The goal of this study was to investigate the prevalence and antimicrobial resistance of ESBL-producing E. coli in small-scaled poultry farms and retail chicken. The cultured E. coli isolates were subjected to phenotypic tests, susceptibility tests, and the polymerase chain reaction for detection of bla CTX-M , bla SHV , and bla TEM genes. From 120 samples each of chicken feces, retail chicken, soil and chicken feed, ESBL-producing E. coli isolates were detected in 75.9%, 63.6%, 39.2%, and 13.3% of the samples, respectively. Minimum inhibitory concentration (MICs) values indicated that ESBL-producing E. coli were resistance to ampicillin (MIC ≥ 32 μg/mL), gentamicin (MIC ≥ 16 μg/mL), cefotaxime (MIC ≥ 4 μg/mL) and ceftriaxone (MIC ≥ 4 μg/mL), respectively. The total resistance for imipenem was also observed at 1.0% (MIC ≥ 4 μg/mL) and none of the isolates were resistant to ceftazidime (MIC ≥ 16 μg/mL). ESBL-producing E. coli from chicken feces and retail chicken carried bla SHV gene at a rate of 6.8% and 5.7%, respectively and bla CTX-M gene was also revealed at 2.9% in retail chicken. Moreover, ESBL-producing E. coli isolated from soil harbored bla SHV and bla CTX-M genes at 5%. None of the feed samples yielded ESBLs genes. Twenty three resistance patterns were observed for multi-resistant ESBL-producing E. coli. This study highlights the prevalence of multi-antimicrobial resistant ESBL-producing E. coli in small-scaledpoultry farms and retail chicken, hence the need to review poultry management practices to minimize the occurrence.

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Characterization of Extended-Spectrum β-Lactamase–Producing Escherichia coli Isolates from Pigs and Farm Workers

Cited by 16 publications

References 26 publications

Characteristics of Extended-Spectrum Beta-Lactamase-Producing Enterobacteriaceae and Contact to Animals in Estonia

Characteristics of Extended-Spectrum Beta-Lactamase-Producing Enterobacteriaceae and Contact to Animals in Estonia

Occurrence of bla _KPC-2 , bla _CTX-M , and mcr-1 in Enterobacteriaceae from Well Water in Rural China

Multiple Antimicrobial Resistance of Extended Spectrum Beta-Lactamase-Producing Escherichia coli from Small-Scaled Poultry Farms and Retail Chicken

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Characterization of Extended-Spectrum β-Lactamase–Producing Escherichia coli Isolates from Pigs and Farm Workers

Cited by 16 publications

References 26 publications

Characteristics of Extended-Spectrum Beta-Lactamase-Producing Enterobacteriaceae and Contact to Animals in Estonia

Characteristics of Extended-Spectrum Beta-Lactamase-Producing Enterobacteriaceae and Contact to Animals in Estonia

Occurrence of bla KPC-2 , bla CTX-M , and mcr-1 in Enterobacteriaceae from Well Water in Rural China

Multiple Antimicrobial Resistance of Extended Spectrum Beta-Lactamase-Producing Escherichia coli from Small-Scaled Poultry Farms and Retail Chicken

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Product

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Occurrence of bla _KPC-2 , bla _CTX-M , and mcr-1 in Enterobacteriaceae from Well Water in Rural China