Characterization of Ethiopian Wheat Germplasm for Resistance to Four <i>Puccinia graminis</i> f. sp. <i>tritici</i> Races Facilitated by Single-Race Nurseries

Hundie, Bekele; Girma, Bedada; Tadesse, Zerihun; Edae, Erena A.; Olivera, Pablo D.; Abera, Endale Hailu; Bulbula, Worku Denbel; Abeyo, Bekele; Badebo, Ayele; Cisar, G.; Brown‐Guedira, Gina; Gale, S.; Jin, Yue; Rouse, Matthew N.

doi:10.1094/pdis-07-18-1243-re

Cited by 21 publications

(22 citation statements)

References 45 publications

(29 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The durum genotypes were all resistant to race TRTTF. In contrast to TRTTF, race JRCQC that is adapted to durum wheat (Hundie et al, 2019) in Ethiopia was the most virulent race on the durum genotypes in our study. This suggests that Sr genes/alleles effective to races TTKSK, TTKST, TTKTT, TKTTF, and TRTTF do not provide resistance to JRCQC.…”

Section: Stem Rust Resistance In Durum Wheat Genotypescontrasting

confidence: 63%

See 1 more Smart Citation

Genome-Wide Association Studies Reveal All-Stage Rust Resistance Loci in Elite Durum Wheat Genotypes

et al. 2021

Self Cite

View full text Add to dashboard Cite

Leaf rust, caused by Puccinia triticina (Pt), stripe rust caused by Puccinia striiformis f. sp. tritici (Pst), and stem rust caused by Puccinia graminis f. sp. tritici (Pgt) are major diseases to wheat production globally. Host resistance is the most suitable approach to manage these fungal pathogens. We investigated the phenotypic and genotypic structure of resistance to leaf rust, stem rust, and stripe rust pathogen races at the seedling stage in a collection of advanced durum wheat breeding lines and cultivars adapted to Upper Mid-West region of the United States. Phenotypic evaluation showed that the majority of the durum wheat genotypes were susceptible to Pt isolates adapted to durum wheat, whereas all the genotypes were resistant to common wheat type-Pt isolate. The majority of genotypes were resistant to stripe rust and stem rust pathogen races. The durum panel genotyped using Illumina iSelect 90 K wheat SNP assay was used for genome-wide association mapping (GWAS). The GWAS revealed 64 marker-trait associations (MTAs) representing six leaf rust resistance loci located on chromosome arms 2AS, 2AL, 5BS, 6AL, and 6BL. Two of these loci were identified at the positions of Lr52 and Lr64 genes, whereas the remaining loci are most likely novel. A total of 46 MTAs corresponding to four loci located on chromosome arms 1BS, 5BL, and 7BL were associated with stripe rust response. None of these loci correspond to designated stripe rust resistance genes. For stem rust, a total of 260 MTAs, representing 22 loci were identified on chromosome arms 1BL, 2BL, 3AL, 3BL, 4AL, 5AL, 5BL, 6AS, 6AL, 6BL, and 7BL. Four of these loci were located at the positions of known genes/alleles (Sr7b, Sr8155B1, Sr13a, and Sr13b). The discovery of known and novel rust resistance genes and their linked SNPs will help diversify rust resistance in durum wheat.

show abstract

Section: Stem Rust Resistance In Durum Wheat Genotypescontrasting

confidence: 63%

“…Five seedlings per line were planted per replicate for all six Pgt races. The inoculum preparation, inoculation, greenhouse conditions, and disease screening were as described by Hundie et al (2019). In brief, the urediniospores stored at -80 • C were heat shocked at 45 • C for 15 min.…”

Section: Stem Rust Phenotypingmentioning

confidence: 99%

Genome-Wide Association Studies Reveal All-Stage Rust Resistance Loci in Elite Durum Wheat Genotypes

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…The races were evaluated in a greenhouse for seedling responses at the USDA‐ARS Cereal Disease Laboratory, St. Paul, MN according to methods described by Hundie et al. (2019). Race TTKSK was screened both in the greenhouse under a normal temperature (19–22 °C), and a cold temperature regime (15 °C to 18 °C, night/day, with a 16 h photoperiod) in a growth chamber.…”

Section: Methodsmentioning

confidence: 99%

Association mapping of resistance to emerging stem rust pathogen races in spring wheat using genotyping‐by‐sequencing

Edae

Rouse

2020

The Plant Genome

Self Cite

View full text Add to dashboard Cite

The identification and characterization of resistance genes should outpace the rapid emergence of new P. graminis f. sp. tritici races, such as TTRTF and TTKTT, to mitigate stem rust damage to wheat. The objective of the current study was to identify and characterize P. graminis f. sp. tritici race resistance association signals. A total of 250 North American spring wheat lines were evaluated at the seedling stage with a total of seven isolates including TKKTP, TKTTF, TKTTF, TRTTF, TTRTF, TTKSK, and TTKTT. The lines were genotyped by a GBS platform and 9,042 SNPs were used for identification of chromosome regions associated with resistance against the seven isolates. Strong association signals were detected on chromosomes 6BL (Sr11 gene region) and 4AL, likely Sr7a, for resistance against both TKKTP and TKTTF. Similarly, association signals were also detected on chromosomes 4AL (race TTRTF resistance) and 4BS (race TTKSK and TTKTT resistance). Association analysis based on mean phenotypic differences between closely related isolates identified QTL that were not elucidated by direct association mapping of the responses, individually. Overall, with the exception of race TRTTF, each race shared at least one association signal with another race. However, the number of race-specific association signals are larger than that of association signals common among races suggesting the need for identifying and characterizing QTL/genes for newly emerging stem rust pathogen races. There was also high concordance between PCA-based GWAS association signals and association signals from that of both single and multilocus mixed models.

show abstract

“…In a 2‐yr field study with the Ug99 group race PTKST, mean yield loss of the susceptible check cultivar was 47.9%, while mean yield loss of Kingbird was 10.1% (Soko, Bender, Prins, & Pretorius, 2018). Kingbird's field performance in Ethiopia against races TTKSK, TKTTF, TRTTF, and JRCQC was very good, with stem rust severities ranging from trace to 15% (Borlaug Global Rust Initiative, 2019; Hundie et al., 2019). We report here the release of three hard white winter wheat germplasm lines, KS14U6380R5 (Reg.…”

Section: Introductionmentioning

confidence: 99%

Registration of hard white winter wheat germplasms KS14U6380R5, KS16U6380R10, and KS16U6380R11 with adult plant resistance to stem rust

Guttieri

Bowden

Reinhart

et al. 2020

J of Plant Registrations

Self Cite

View full text Add to dashboard Cite

Resistance to the Ug99 group of races of the stem rust fungus Puccinia graminis f. sp. tritici is limited in winter wheat (Triticum aestivum L.) germplasm adapted to the Great Plains of the United States. Our objective was to generate regionally adapted hard winter wheat germplasm with combinations of adult plant resistance genes that are expected to provide durable resistance. KS14U6380R5 (Reg. no. GP-1043, PI 689115), KS16U6380R10 (Reg. no. GP-1044, PI 689116), and KS16U6380R11 (Reg. no. GP-1045, PI 689117) were derived from backcrosses of the hard white winter wheat germplasm KS05HW14 to the stem rust-resistant Kenyan spring wheat cultivar 'Kingbird'. KS14U6380R5, KS16U6380R11, and KS16U6380R10 were developed by pedigree selection and were initially evaluated as U6380-11-2R-0A, U6380-210-2R-0A, and U6380-148-4R-2T, respectively. The germplasms were developed by the USDA-ARS and jointly released with the Kansas State University Agricultural Experiment Station. These germplasms provide parents for development of hard winter wheat cultivars with durable resistance to stem rust.

show abstract

Characterization of Ethiopian Wheat Germplasm for Resistance to Four Puccinia graminis f. sp. tritici Races Facilitated by Single-Race Nurseries

Cited by 21 publications

References 45 publications

Genome-Wide Association Studies Reveal All-Stage Rust Resistance Loci in Elite Durum Wheat Genotypes

Genome-Wide Association Studies Reveal All-Stage Rust Resistance Loci in Elite Durum Wheat Genotypes

Association mapping of resistance to emerging stem rust pathogen races in spring wheat using genotyping‐by‐sequencing

Registration of hard white winter wheat germplasms KS14U6380R5, KS16U6380R10, and KS16U6380R11 with adult plant resistance to stem rust

Contact Info

Product

Resources

About