Characterization of esterase activity from an Acetomicrobium hydrogeniformans enzyme with high structural stability in extreme conditions

Kumagai, Patricia S.; Gutierrez, Raissa Ferreira; Lopes, José Luiz de Souza; Martins, Julia M.; Jameson, David M.; Castro, Aline Machado de; Martins, Luiz Fernando; DeMarco, Ricardo; Bossolan, Nelma Regina Segnini; Wallace, B.A.; Araújo, Ana Paula Ulian de

doi:10.1007/s00792-018-1038-3

Cited by 13 publications

(5 citation statements)

References 49 publications

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“…6a). Similar or even lower solvent tolerance was found for most of the reported OST esterases (Doukyu and Ogino 2010;Xing et al 2012;Zhang et al 2014;Kumagai et al 2018). Thus, in comparison, Est2 exhibited superior stability against higher concentrations of the analyzed water-miscible organic solvents (Fig.…”

Section: Discussionsupporting

confidence: 68%

“…However, the inhibition or inactivation of enzyme activity resulting from organic solvents has restricted the use of many lipolytic enzymes (Klibanov 2001;). To overcome this limitation, some organic solvent-tolerant (OST) lipolytic enzymes have been isolated, including enzymes from Bacillus licheniformis S-86 (Torres et al 2009), Streptomyces coelicolor A3(2) , Psychrobacter celer 3Pb1 , Alcanivorax dieselolei B-5(T) (Zhang et al 2014), and Acetomicrobium hydrogeniformans (Kumagai et al 2018), as well as from metagenomes of seawater , compost , lipid-contaminated soil , mountain soil , swamp sediment , and deep-sea hydrothermal vents ). However, these enzymes only show tolerance towards specific organic solvents.…”

Section: Conflicts Of Interestmentioning

confidence: 99%

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Metagenomic approaches to discover lipolytic enzymes

Lu¹

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Stable in a Microorganism Pseudomonas aeruginosa san-ai 30 °C, 48 h 25 % (v/v) chloroform and n-hexane Karadzic et al. 2006 Bacillus sphaericus 205y 37 °C, 30 min 25 % (v/v) n-hexane and p-xylene. Hun et al. 2003 Bacillus megaterium 29 °C, 1 h 25-80 % (v/v) ethanol and acetone 100 % (v/v) 2-propanol, 1-butanol, Tol, n-hexane and n-heptane Lima et al. 2004 Sulfolobus solfataricus P1 30 °C or 70 °C, 1 h 40 % (v/v) methanol, ethanol and 2propanol. Mandrich et al. 2005 Stenotrophomonas maltophilia CGMCC 4254 30 °C, 24 h 20 and 50 % (v/v) benzene, toluene, nhexane and n-heptane. Li et al. 2013 Pseudomonas aeruginosa MH38 25 °C, 1 h 30 and 50 % (v/v) benzene and hexane Jang et al. 2014 Monascus purpureus 40 °C, 24 h 20 % (v/v) methanol, ethanol, acetonitrile, glycerol, acetone, n-Hexane, toluene and chloroform Kang et al. 2017 Chromohalobacter sp. 35 °C, 30 min 20 and 50 % (v/v) benzene and hexane Ai et al. 2018 Psychrobacter sp. ZY124 37 °C, 5 h 10, 30 and 50 % (v/v) DMSO, methanol, ethanol, acetone, acetonitrile, toluene, pentane, hexane and octane Zhang et al. 2018b Metagenome Marine mud 30 °C, 12 h 20 % (v/v) ethanol, acetonitrile, DMF and cyclohexane Gao et al. 2016 Forest soil sample. 37 °C, 2 h 25 % (v/v) DMSO, Benzene and p-xylene Berlemont et al. 2013 Wastewater treatment plant of a meat packing and dairy industry 4 °C, 48 h 15 and 30 % (v/v) methanol, ethanol, 1propanol, 2-propanol, glycerol, THF, dioxane, DMSO Glogauer et al. 2011 Soil 30 °C, 2 h 15 and 30 % (v/v) DMSO, DMF, p-xylene, hexane, heptane, and octane Wang et al. 2013 Compost Room temp., 26 d 30 % (v/v) methanol, ethanol, isopropanol, DMSO, acetone Lu et al., 2019 Soil contaminated with petroleum hydrocarbons No data 30 % (v/v) DMF and DMSO Pereira et al. 2015 a Abbreviations for the organic solvents are: DMF, N,N-dimethylformamide; DMSO, dimethylsulfoxide; THF, tetrahydrofuran. Halotolerance Among extremophilic LEs, halophilic/halotolerant LEs are another major group of industrial relevant enzymes. In comparison to the non-halophilic/non-halotolerant Source of LEs Enzyme properties Reference Salt range Maximum activity (%) a Microorganism Pelagibacterium halotolerans 0-4 M ~ 160 % at 3 M Jiang et al. 2012 Alcanivorax borkumensis 0-3.5 M 100 % at 0 M Tchigvintsev et al. 2015 Serratia sp. 0-4 M 100 % at 0 M Jiang et al. 2016 Alkalibacterium sp. 0-4 M ~ 105 % at 2 M Wang et al. 2016 Psychrobacter pacificensis 0-5 M 143.2 % at 2 M Wu et al. 2013a Lactobacillus plantarum 0-25 % ~ 250 % at 1M Esteban-Torres et al. 2014 Zunongwangia profunda 0-4.5 M 100 % at 0 M Rahman et al. 2016 Haloarcula marismortui 0-5 M 800 mU at 3 M Rao et al. 2009 Thalassospira sp. 0-4 M 283 % at 3 M De Santi et al. 2016b Bacillus licheniformis 0-5 M 588 % at 3.5 M Zhang et al. 2018 Metagenome Deep sea sponge 0-24% 100 % at 0 M Borchert et al. 2017 Marine sponge 0-4 M 234 % at 5 M Selvin et al. 2012 Soil 0-5 M 155 % at 1 M Jayanath et al. 2018 Marine arctic sediment 0-4 M 675 % at 3 M De Santi et al. 2016a Deep-sea shrimp 0-4 M ~ 250 % at 3.2 M Alcaide et al. 2015b 0-4 M ~ 250 %...

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Section: Discussionsupporting

confidence: 68%

Section: Conflicts Of Interestmentioning

confidence: 99%

Metagenomic approaches to discover lipolytic enzymes

Lu¹

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“…Thermovirga and norank_f__Synergistaceae were both reported to be capable of fermenting proteins, amino acids, and some other small-molecular organic acids [4], while Aminobacterium could be able to ferment amino acids and organic acids into VFAs [39]. Acetomicrobium was reported to possess the ability to secrete thermophilic esterases for the hydrolysis of esters into small-molecular organic acids [45]. Additionally, Coprothermobacter (3.97%), affiliated with Coprothermobacteraeota, and Corynebacterium (1.21%), affiliated with Actinobacteria, were also reported to be capable of degrading proteins and complex organic matters to small-molecular organics.…”

Section: Microbial Community In the Hydrolytic/acidogenic Processmentioning

confidence: 99%

Composition Characterization and Transformation Mechanism of Dissolved Organic Matters in a Full-Scale Membrane Bioreactor Treating Co-Digestion Wastewater of Food Waste and Sewage Sludge

et al. 2022

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The membrane bioreactor (MBR) serves as the most widely used technology in anaerobic digestion wastewater treatment, but the composition and transformation of the dissolved organic matters (DOMs) are vague. This study focused on the composition characterization and transformation mechanism of DOMs in real co-digestion wastewater of food waste and sewage sludge from a full-scale MBR via molecular weight cut-off, 3D-EEM, FT-IR, and SPME-GC/MS. The results indicated that the co-digestion wastewater mainly comprised organics with molecular weight (MW) lower than 1 kDa and dominated by tryptophane-protein-like substances. The hydrolytic/acidogenic process improved the biodegradability with the conversion of high-MW organics into low-MW organics, while the two-stage A/O process possessed the highest contribution to the organic removal with the consumption of most DOMs. However, the deficient removal of refractory organics (MW < 5 kDa) in the ultrafiltration unit led to the residual DOMs in the effluent. The potential functional bacteria in the biological processes have also been identified and were principally affiliated with Proteobacteria and Firmicutes. These findings could help to advance the understanding of the co-digestion wastewater and provide fundamental information for the optimization and development of MBR in anaerobic digestion wastewater treatment.

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“…Enzyme enhanced oil recovery (EEOR) refers to any enhanced oil recovery technologies that involves using enzyme(s). Comparing to the lab studies and field pilot tests of regular EOR methods aforementioned, EEOR is an emerging technology that has limited peer reviewed papers [6][7][8][9][10][11][12], yet huge amount of conference papers [22,26,30] , and unpublished technical reports and case studies [24,31]. Encouraged by the promising field performance, the rich data of industrial reports, and the potential of minimum footprints,…”

Section: Introductionmentioning

confidence: 99%

Green Enzyme Enhanced Oil Recovery

Zeng¹

2021

GJES

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Enhanced oil recovery (EOR) is widely performed in oil fields after primary and secondary productions. Enzyme enhanced oil recovery (EEOR) has seen rapid growth in both lab research and field applications in recent years due to its promising effectiveness and minimum footprint to the environment in comparing to other EOR methods. To promote the progress of this emerging technology, this preliminary review is conducted with the intention of revealing and sharing experience from many unpublished case studies, and integrating them with results in conference and peer reviewed papers, with special attention to the application of green enzyme, a DNA-modified protein. The collected literature and technical reports included cases from Asia, Middle East, South America and USA. Mechanisms to the success of some cases are discussed. Factors limiting the applications in some cases are shared. Directions of future efforts are proposed.

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Characterization of esterase activity from an Acetomicrobium hydrogeniformans enzyme with high structural stability in extreme conditions

Cited by 13 publications

References 49 publications

Metagenomic approaches to discover lipolytic enzymes

Metagenomic approaches to discover lipolytic enzymes

Composition Characterization and Transformation Mechanism of Dissolved Organic Matters in a Full-Scale Membrane Bioreactor Treating Co-Digestion Wastewater of Food Waste and Sewage Sludge

Green Enzyme Enhanced Oil Recovery

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