Characterization of Escherichia coli isolated from foals

Holland, Robert E.; Schmidt, Amelia K; Sriranganathan, N.; Grimes, Sheila; Wilson, Richard A.; Brown, C. M.; Walker, Robert D.

doi:10.1016/0378-1135(95)00162-x

Cited by 24 publications

(30 citation statements)

References 32 publications

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“…Escherichia coli ( E. coli ), despite being part of the normal flora of the gastrointestinal tract of foals and other animals [8], is also known to be an important aetiological agent of foal diarrhoea [9]. The presence of virulence markers in infecting strains of E. coli has been demonstrated to be responsible for the pathogenicity detected in infected foals [9, 10].…”

Section: Introductionmentioning

confidence: 99%

Prevalence and Characteristics of Enteric Pathogens Detected in Diarrhoeic and Non-Diarrhoeic Foals in Trinidad

Harris

Sankar

Small

et al. 2012

Veterinary Medicine International

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The study determined the relative importance of Escherichia coli, E. coli O157, Salmonella spp., Clostridium spp., rotavirus, Cryptosporidium spp., and Strongyloides westeri in foal (diarrhoeic and non-diarrhoeic) available for sampling during the foaling season of 2010 and determined their sensitivity to antimicrobial agents. A cross-sectional study was conducted on 164 foals (9 diarrhoeic and 155 non-diarrhoeic) from 15 farms in Trinidad. Isolation and detection of enteric pathogens followed standard methods, and the antibiograms of E. coli and Salmonella spp. were determined using the disc diffusion method. All organisms investigated were detected except E. coli O157. A high prevalence of E. coli (85.0%), Cryptosporidium spp. (64.8%), Strongyloides westeri (35.7%) was seen, but the prevalence was comparatively low for Clostridium spp. (12.9%), Salmonella spp. (4.4%) and rotavirus (2.1%). Only Salmonella spp. was isolated at a statistically significantly ( P < 0.05; χ 2 ) higher frequency from diarrhoeic (25.0%) than non-diarrhoeic (4.0%) foals. Amongst E. coli isolates, the frequency of resistance was higher in isolates from diarrhoeic compared with non-diarrhoeic foals but the difference was only statistically significant ( P < 0.05; χ 2 ) for tetracycline. All isolates of Salmonella spp. were sensitive to streptomycin and sulphamethoxazole/trimethoprim, a finding that may have therapeutic significance.

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Section: Introductionmentioning

confidence: 99%

Prevalence and Characteristics of Enteric Pathogens Detected in Diarrhoeic and Non-Diarrhoeic Foals in Trinidad

Harris

Sankar

Small

et al. 2012

Veterinary Medicine International

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“…An O86 isolate was reported in a group of E. coli strains collected from horses (Holland et al 1996).…”

Section: ------------------------------------------------------------mentioning

confidence: 99%

Isolation of Escherichia coli O86:K61 producing cyto‐lethal distending toxin from wild birds of the finch family

Foster¹,

Ross²,

Tw³

et al. 1998

Letters in Applied Microbiology

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Escherichia coli was recovered from selected tissues of 43 out of a total of 46 finches found dead in the Scottish Highlands during April–May of 1994 and 1995. The isolates did not ferment sorbitol, rhamnose, sucrose or melibiose; they belonged to serogroup O86:K61, produced cytolethal distending toxin (CLDT) and possessed the eae gene sequence. The consistent recovery of organisms producing CLDT and possessing the eae gene suggests that these organisms may have played a significant role in the finch mortalities.

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“…However, the evidence was probe based, and no studies were done to test for the presence of large plasmids in the O86:K61 isolates. It would be valuable to compare the isolates described here with isolates derived from humans, calves, pigs, and chickens (3,12,32,56).…”

Section: Vol 68 2002 E Coli Isolates Possessing Gamma-like Intiminmentioning

confidence: 99%

“…coli O86:K61 has been associated with diarrheal disease in calves (12,56), pigs (3), and horses (32) and on one occasion has been implicated in cellulitis in broiler chickens (47). To date, this serotype has not been isolated from commercial poultry (Rob Davies, personal communication).…”

mentioning

confidence: 99%

Phenotypic and Genotypic Characterization of Avian Escherichia coli O86:K61 Isolates Possessing a Gamma-Like Intimin

Ragione

McLaren

Foster³

et al. 2002

Appl Environ Microbiol

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Escherichia coli O86:K61 has long been associated with outbreaks of infantile diarrhea in humans and with diarrheal disease in many animal species. Studies in the late 1990s identified E. coli O86:K61 as the cause of mortality in a variety of wild birds, and in this study, 34 E. coli O86:K61 isolates were examined. All of the isolates were nonmotile, but most elaborated at least two morphologically distinct surface appendages that were confirmed to be type 1 and curli fimbriae. Thirty-three isolates were positive for the eaeA gene encoding a gamma type of intimin. No phenotypic or genotypic evidence was obtained for elaboration of Shiga-like toxins, but most isolates possessed the gene coding for the cytolethal distending toxin. Five isolates were selected for adherence assays performed with tissue explants and HEp-2 cells, and four of these strains produced attaching and effacing lesions on HEp-2 cells and invaded the cells, as determined by transmission electron microscopy. Two of the five isolates were inoculated orally into 1-day-old specific-pathogen-free chicks, and both of these isolates colonized, invaded, and persisted well in this model. Neither isolate produced attaching and effacing lesions in chicks, although some pathology was evident in the alimentary tract. No deaths were recorded in inoculated chicks. These findings are discussed in light of the possibility that wild birds are potential zoonotic reservoirs of attaching and effacing E. coli.Escherichia coli O86:K61 has long been associated with outbreaks of infantile diarrhea in humans (24). This serotype is classified historically as enteropathogenic E. coli (EPEC) along with serotypes O55, O111, and O127 among others and has been implicated as a major cause of acute and persistent infantile diarrhea in developing parts of the world (39). Smallbowel biopsies of children infected with EPEC revealed discrete colonies of bacteria attached to the mucosa (58). Binding of EPEC to the brush border triggers a cascade of transmembrane and intracellular signals leading to cytoskeletal reorganization and formation of a specific lesion, termed an attaching and effacing (A/E) lesion, which involves intimate bacterial attachment to the host cell and associated effacement of microvilli (43). Jerse et al. (34) first described the eaeA gene of EPEC serotype O127:H6 that encodes intimin, a surfacearrayed protein essential for intimate association with enterocytes. Subsequent analysis showed that this gene is one of a cluster of over 40 genes within the locus of enterocyte effacement (LEE) that encode a type III secretion system essential for A/E lesion formation (45). EPEC possess plasmid-encoded factors, such as the bundle-forming pili (bfp) and the plasmidencoded regulator (per), which also play roles in intimate adherence of EPEC (45). Similar A/E lesions are produced by enterohemorrhagic E. coli (EHEC) (51, 55), Hafnia alvei (2), Citrobacter rodentium (10), and rabbit diarrheagenic E. coli (15). The deduced amino acid sequences of the EaeA protein products of...

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Characterization of Escherichia coli isolated from foals

Cited by 24 publications

References 32 publications

Prevalence and Characteristics of Enteric Pathogens Detected in Diarrhoeic and Non-Diarrhoeic Foals in Trinidad

Prevalence and Characteristics of Enteric Pathogens Detected in Diarrhoeic and Non-Diarrhoeic Foals in Trinidad

Isolation of Escherichia coli O86:K61 producing cyto‐lethal distending toxin from wild birds of the finch family

Phenotypic and Genotypic Characterization of Avian Escherichia coli O86:K61 Isolates Possessing a Gamma-Like Intimin

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