Characterization of Erwinia chrysanthemi isolates inciting stalk rot disease of sorghum

Kharayat, Bhupendra Singh; Singh, Yogendra

doi:10.5897/ajar2014.9031

Cited by 4 publications

(1 citation statement)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Pectobacterium spp. proven to have the pectate degrading ability (Bhupendra and Yogendra, 2015), similarly, in the present study the isolates Ikg 2 and Icb 4 were found to degrade the sodium polypectate present in the CVP medium, leading to the formation of fissures in five days. Bacterial isolates were insensitive to erythromycin but sensitive to streptomycin.…”

Section: Kbsupporting

confidence: 81%

Molecular Detection of the Causative Agent of Soft Rot (Pectobacterium carotovorum subsp carotovorum) in Banana (Musa sp.)

Ragavi¹,

Muthamilan²,

Nakkeeran³

et al. 2019

Int.J.Curr.Microbiol.App.Sci

View full text Add to dashboard Cite

Soft rot disease of Banana caused by Pectobacterium carotovorum subsp. Carotovorum is found to be a serious disease and cause considerable yield loss. Common cultivars viz., Grand Naine, Rasthali and Nendran are extremely prone to this disease. Understanding the disease diagnosis and pathogen virulence is important in management programs. The pathogen was isolated from infected rhizomes from five locations of Theni district of Tamil Nadu. Pathogenicity of all isolates was confirmed by in vitro inoculation in rhizome bits and in vivo inoculation in pseudostems of three months old tissue cultured plants of cv. Grand Naine. The virulent isolates Icb 4 and Ikg 2 were characterized according to the morphological and microscopic characteristics, along with the physiological and biochemical assays; which indicates that the bacteria are gram negative, rod shaped, peritrichously flagellated. The colonies form characteristic deep cavities or pits in Crystal violet pectate medium (CVP). Results of biochemical tests showed that isolates Icb 4 and Ikg 2 have a positive reaction for catalase, H 2 S production and nitrate reductase and a negative reaction for oxidase test, Indole test and gelatin liquefaction. The results determining the optimal temperature for the growth of isolates Icb 4, and Ikg 2 have revealed that the temperature at 30 ºC -37 ºC were suitable to obtain profuse growth of Pectobacterium. Total DNA extracted from the isolates Icb 4, and Ikg 2 were subjected to PCR analysis of 16S-23S intergenic transcribed spacer region. PCR amplified 1.5 kb amplicons were sequenced. Sequence Homology and phylogeny analyses had shown that the isolates Icb 4 and Ikg 2 were belonged to Pectobacterium carotovorum subsp. carotovorum.

show abstract

Section: Kbsupporting

confidence: 81%