Characterization of eosinophilic hyaline droplets in schwannoma

Yokoo, Hideaki; Arai, Hanako; Isoda, Koji; Arai, Miho; Sasaki, Atsushi; Hirato, Junko; Nakazato, Yoichi

doi:10.1007/s00401-002-0631-8

Cited by 10 publications

(12 citation statements)

References 9 publications

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“…The occurrence of densely eosinophilic, fine or coarse intracytoplasmic granules or droplets has been frequently described in various neoplasms of the central and peripheral nervous systems, such as astrocytoma, 1–5 glioblastoma, 3,4,6–8 oligodendroglioma, 9,10 medulloblastoma 11 and schwannoma 12,13 . However, to our knowledge previous descriptions of similar intracytoplasmic granules in ependymoma have been limited to a few reports 14,15 .…”

Section: Discussionmentioning

confidence: 98%

“…Intracytoplasmic granules in granular cells have various ultrastructural substrates which differ from case to case. In most examples, a granular cell change was the result of an accumulation of autophagolysosomes or residual bodies, and most of the reported cases of astrocytoma, 3,4 glioblastoma, 3,4,6,8 oligodendroglioma, 9 medulloblastoma 11 and schwannoma 12,13 showing granular cell features belong to this category. This accumulation of autophagolysosomes is essentially similar to that seen in granular cell tumors which originate from Schwann cells, 18 and in cases of granular cell tumor and schwannoma, Shintaku demonstrated that they are immunoreactive for LC3, a protein specifically localized to the autophagosomal membrane 13 .…”

Section: Discussionmentioning

confidence: 99%

“…The occurrence of intracytoplasmic, eosinophilic, often refractile granules has been known in various neoplasms. Among neoplasms of the nervous system, astrocytoma, 1–5 glioblastoma, 3,4,6–8 oligodendroglioma, 9,10 medulloblastoma 11 and schwannoma 12,13 occasionally contain these intracytoplasmic granules. According to the descriptions in the literature, they are variable in histopathological, immunohistochemical and ultrastructural features, and their origin and pathogenesis are probably heterogeneous.…”

Section: Introductionmentioning

confidence: 99%

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Ependymoma with “granular cell” features: Report of two cases

Shintaku

Yoshida²,

Ikarashi³

et al. 2012

Neuropathology

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We report two cases of ependymoma which showed prominent “granular cell” changes of the cytoplasm. The patients were a 7‐year‐old boy with a tumor in the cerebellum (case 1) and a 70‐year‐old man with a tumor in the frontal lobe (case 2). The tumor of case 1 showed a histopathological appearance of ependymoma containing many focal aggregates of large polygonal cells in which the cytoplasm was stuffed with numerous eosinophilic granules. The tumor of case 2 predominantly showed the features of papillary ependymoma, and some tumor cells were swollen and contained similar eosinophilic granules. Intracytoplasmic granules in both tumors were immunoreactive for GFAP and ubiquitin, but not for epithelial membrane antigen, CD68 or mitochondria. Ultrastructurally, they were found as aggregates of membrane‐bound, electron‐dense, globular structures. Karyotypic analysis of the tumor in case 1 demonstrated 2, 11 and 12 trisomies. Intracytoplasmic eosinophilic granules occasionally occur in astrocytic and oligodendroglial neoplasms, but an appearance of similar granules is very rare in ependymoma. The two cases presented here may represent a new histopathological variant of ependymoma, and the term “granular cell ependymoma” is appropriate for them.

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Ependymoma with “granular cell” features: Report of two cases

Shintaku

Yoshida²,

Ikarashi³

et al. 2012

Neuropathology

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“…In our previous article we described bright, eosinophilic and occasionally large globules in schwannomas and named them eosinophilic hyaline droplets (EHD) 3 . They occur in 7% of all schwannomas, but they appear in nearly 20% of acoustic schwannomas.…”

Section: Introductionmentioning

confidence: 99%

Oxidative stress is related to the formation of Antoni B patterns and eosinophilic hyaline droplets in schwannomas

et al. 2007

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Schwannomas, particularly of vestibular origin, often accompany degenerative hypocellular areas known as Antoni B patterns; however, the detailed mechanism is uncertain. Eosinophilic hyaline droplets (EHD), the substantial nature of which are autophagic vacuoles, preferentially appear in acoustic schwannomas and distribute around areas of Antoni B. We investigated their common background using schwannomas with (15 cases) or without (10 cases) EHD, and demonstrated that EHD showed selective immunoreactivity with an anti-nitrotyrosine antibody, suggesting the overproduction of nitric oxide in this condition. The expression of inducible nitric oxide synthase was emphasized in infiltrating macrophages around hyalinized vessels. Protein-bound 4-hydroxy 2-nonenal, another oxidative stress marker, was detected in Antoni B tissue, but not in EHD. Antibodies to cleaved caspase-3 and single strand DNA, indicators of apoptosis, did not label tumors cells in Antoni B areas as well as EHD-bearing cells. The morphology and the mitotically static state of EHD-laden cells are phenotypically similar to autophagic cell death; however, autophagy in normal cells is a cell survival strategy against starvation, so the possibility remains that EHD are formed in that context. In either case, schwannomas may show a characteristic autophagic change by an endogenous mechanism. Tumor growth in a narrow intracranial space and resultant ischemia by self-oppression were postulated to be an initial event, because ischemia-reperfusion injury is a major source of reactive oxygen species and ischemia is also a potent trigger of autophagy as well as of tissue degeneration. Moreover, potential roles of chemokines and hemosiderosis are discussed.

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“…Yokoo et al indicated that oxidative stress could provoke regressive histopathology in schwannomas. 13,14 Cellular degeneration associated with iron deposition has been also indicated in neurodegenerative diseases, 15,16 indicating that regressive findings in meningiomas are associated with iron-derived oxidative stress.…”

Section: Discussionmentioning

confidence: 99%

Cytoplasmic iron deposition is associated with the expression of oxidative DNA damage marker in meningiomas

et al. 2013

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Angiomatous meningiomas are rare meningioma subtypes, which are characterized by abundant, well‐formed vessels. We encountered two cases of newly diagnosed angiomatous meningiomas exhibiting tumor cells with brown pigments, which were histochemically proven to be iron. In an attempt to understand its pathological significance, we assessed this unusual finding in representatives for each grade of meningiomas and immunoexpression of transferrin receptor (CD71) and the oxidative DNA damage marker, 8‐hydroxy‐2′‐deoxyguanosine (8‐OHdG). Iron deposition in the tumor cells was observed in 8/15 (53%) angiomatous meningioma cases, 2/6 (33%) microcystic meningiomas and 2/20 (10%) meningothelial meningiomas, which included clustered microvessels, but not in fibrous, atypical or anaplastic meningiomas (P = 0.001). Cytoplasmic CD71 expression was largely negative in angiomatous meningioma cases, but positive in meningothelial and high‐grade meningiomas, suggesting that the transferrin‐dependent iron transporter was involved in iron uptake in meningiomas. Nuclear expression of 8‐OHdG was observed in ≥50% of the tumor cells in all 15 cases of angiomatous meningioma and was associated with the presence of regressive histopathological findings, such as hyalinized vessels and cystic changes. In addition, the fraction of iron‐containing tumor cells was correlated to those expressing 8‐OHdG (P = 0.005). Our finding indicates that cytoplasmic iron deposition in tumor cells is characteristic of highly vascularized benign meningiomas and related to increased oxidative DNA damage markers.

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Characterization of eosinophilic hyaline droplets in schwannoma

Cited by 10 publications

References 9 publications

Ependymoma with “granular cell” features: Report of two cases

Ependymoma with “granular cell” features: Report of two cases

Oxidative stress is related to the formation of Antoni B patterns and eosinophilic hyaline droplets in schwannomas

Cytoplasmic iron deposition is associated with the expression of oxidative DNA damage marker in meningiomas

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