Characterization of eosinophilic esophagitis murine models using optical coherence tomography

Alex, Aneesh; Noti, Mario; Wojno, Elia D. Tait; Artis, David; Zhou, Chao

doi:10.1364/boe.5.000609

Cited by 9 publications

(2 citation statements)

References 65 publications

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“…The muscularis mucosa (MM) layer between the lamina propria (LP) and SM is a less reflective layer as it predominantly comprises skeletal muscle, while LP and SM exhibit strong scattering and appear as a bright layer since they contain abundant collagen fibers. 21,22 The penetration depth of the 1550-nm band is slightly larger than that of the 1310-nm band, and the axial resolution of the 1310-nm band is obviously better than that of the 1550-nm band, which correlates well with the theoretical prediction. In consequence of the better resolution of the 1310-nm wavelength band, the visibility of LP is better in Fig.…”

Section: Resultssupporting

confidence: 77%

Simultaneous dual-band optical coherence tomography for endoscopic applications

Wei

et al. 2014

J. Biomed. Opt

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Dual-band optical coherence tomography (OCT) can greatly enhance the imaging contrast with potential applications in functional (spectroscopic) analysis. A new simultaneous dual-band Fourier domain mode-locked swept laser configuration for dual-band OCT is reported. It was based on a custom-designed dual-channel driver to synchronize two different wavelength bands at 1310 and 1550 nm, respectively. Two lasing wavelengths were swept simultaneously from 1260 to 1364.8 nm for the 1310-nm band and from 1500 to 1604 nm for the 1550-nm band at an A-scan rate of 45 kHz. Broadband wavelength-division multiplexing was utilized to couple two wavelength bands into a common catheter for circumferential scanning to form dual-band OCT. The proposed dual-band OCT scheme was applied to endoscopic OCT imaging of mouse esophageal wall ex vivo and human fingertip in vivo to justify the feasibility of the proposed imaging technique. The proposed dual-band OCT system is fast and easy to be implemented, which allows for in vivo high-speed biomedical imaging with potential applications in spectroscopic investigations for endoscopic imaging.

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Section: Resultssupporting

confidence: 77%

Simultaneous dual-band optical coherence tomography for endoscopic applications

Wei

et al. 2014

J. Biomed. Opt

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“…Feasibility of OCT as a diagnostic tool has been demonstrated in various pre-clinical animal [24,25] and human [14,[26][27][28][29][30] oral cavity studies. Computational methods for automated OCT image analysis and classification [31], quantitative assessment [28,32] have been developed to aid diagnosis of oral cavity lesions.…”

Section: Introductionmentioning

confidence: 99%

In vivo wide-field reflectance/fluorescence imaging and polarization-sensitive optical coherence tomography of human oral cavity with a forward-viewing probe

Yoon

Jang

Xiao

et al. 2015

Biomed. Opt. Express

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Abstract:We report multimodal imaging of human oral cavity in vivo based on simultaneous wide-field reflectance/fluorescence imaging and polarization-sensitive optical coherence tomography (PS-OCT) with a forward-viewing imaging probe. Wide-field reflectance/fluorescence imaging and PS-OCT were to provide both morphological and fluorescence information on the surface, and structural and birefringent information below the surface respectively. The forward-viewing probe was designed to access the oral cavity through the mouth with dimensions of approximately 10 mm in diameter and 180 mm in length. The probe had field of view (FOV) of approximately 5.5 mm in diameter, and adjustable depth of field (DOF) from 2 mm to 10 mm by controlling numerical aperture (NA) in the detection path. This adjustable DOF was to accommodate both requirements for image-based guiding with high DOF and high-resolution, high-sensitivity imaging with low DOF. This multimodal imaging system was characterized by using a tissue phantom and a mouse model in vivo, and was applied to human oral cavity. Information of surface morphology and vasculature, and under-surface layered structure and birefringence of the oral cavity tissues was obtained. These results showed feasibility of this multimodal imaging system as a tool for studying oral cavity lesions in clinical applications. 281-284 (1996). 6. M. Olivo, R. Bhuvaneswari, and I. Keogh, "Advances in bio-optical imaging for the diagnosis of early oral cancer," Pharmaceutics 3(4), 354-378 (2011). 7 fold imaging with polarization sensitive optical coherence tomography and a MEMS scanning catheter," Opt. ©2015 Optical Society of America

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Label-Free Imaging of Eosinophilic Esophagitis Mouse Models Using Optical Coherence Tomography

Alex

Wojno

Artis

et al. 2016

Methods in Molecular Biology

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Eosinophilic esophagitis (EoE) is an immune-mediated disorder characterized by esophageal inflammation and related structural changes causing symptoms such as feeding difficulties and food impaction. The pathophysiological mechanisms underlying EoE remain poorly understood. Preclinical studies using mouse models have been critical in comprehending human disease mechanisms and associated pathways. In this chapter, we describe an experimental method using a noninvasive label-free optical imaging technique, optical coherence tomography, to characterize the pathophysiological changes in the esophagus of mice with EoE-like disease ex vivo.

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Characterization of eosinophilic esophagitis murine models using optical coherence tomography

Cited by 9 publications

References 65 publications

Simultaneous dual-band optical coherence tomography for endoscopic applications

Simultaneous dual-band optical coherence tomography for endoscopic applications

In vivo wide-field reflectance/fluorescence imaging and polarization-sensitive optical coherence tomography of human oral cavity with a forward-viewing probe

Label-Free Imaging of Eosinophilic Esophagitis Mouse Models Using Optical Coherence Tomography

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