Characterization of environmental Pseudomonas aeruginosa using multilocus sequence typing scheme

Radó, Júlia; Kaszab, Edit; Petrovics, Tünde; Pászti, Judit; Kriszt, Balázs; Szoboszlay, Sándor

doi:10.1099/jmm.0.000589

Cited by 20 publications

(12 citation statements)

References 57 publications

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“…In municipal WWTP activated sludge, three strains showing the capability of degrading nitrofurantoin were found: Rhizobium radiobacter (P4c), Pseudomonas aeruginosa (P4a), and Sphingobacterium thalpophilum (P3d). R. radiobacter has been previously recognized as degrading several dyes [37,38]; the second strain is an ubiquitous environmental bacteria displaying great biodegradation potential [39,40]. However, the strain from the Sphingobacterium thalpophilum genus was found in activated sludge fed with pharmaceutical waste water [41].…”

Section: Resultsmentioning

confidence: 99%

Nitrofurantoin—Microbial Degradation and Interactions with Environmental Bacterial Strains

Pacholak

Smułek

Zgoła‐Grześkowiak

et al. 2019

IJERPH

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The continuous exposure of living organisms and microorganisms to antibiotics that have increasingly been found in various environmental compartments may be perilous. One group of antibacterial agents that have an environmental impact that has been very scarcely studied is nitrofuran derivatives. Their representative is nitrofurantoin (NFT)—a synthetic, broad-spectrum antibiotic that is often overdosed. The main aims of the study were to: (a) isolate and characterize new microbial strains that are able to grow in the presence of NFT, (b) investigate the ability of isolates to decompose NFT, and (c) study the impact of NFT on microbial cell properties. As a result, five microbial species were isolated. A 24-h contact of bacteria with NFT provoked modifications in microbial cell properties. The greatest differences were observed in Sphingobacterium thalpophilum P3d, in which a decrease in both total and inner membrane permeability (from 86.7% to 48.3% and from 0.49 to 0.42 µM min−1) as well as an increase in cell surface hydrophobicity (from 28.3% to 39.7%) were observed. Nitrofurantoin removal by selected microbial cultures ranged from 50% to 90% in 28 days, depending on the bacterial strain. Although the isolates were able to decompose the pharmaceutical, its presence significantly affected the bacterial cells. Hence, the environmental impact of NFT should be investigated to a greater extent.

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Section: Resultsmentioning

confidence: 99%

Nitrofurantoin—Microbial Degradation and Interactions with Environmental Bacterial Strains

Pacholak

Smułek

Zgoła‐Grześkowiak

et al. 2019

IJERPH

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“…Radó et al characterized PA isolated from hydrocarbon or polycyclic aromatic hydrocarbon-contaminated areas using a multi-locus sequence typing (MLST) scheme [66]; they have found isolates belonging to the ST-253 (belonging to the clonal complex PA14, which are high-risk clones with a propensity to become MDR) and ST-198 (associated with CF) sequence types, and seven different serotypes were detected. All isolates carried three virulence genes (exoY, exoT, and exoA), while many carried the additional two genes (exoS and exoU) as well.…”

Section: Discussionmentioning

confidence: 99%

Relationship between Biofilm-Formation, Phenotypic Virulence Factors and Antibiotic Resistance in Environmental Pseudomonas aeruginosa

et al. 2022

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The formation of a protective biofilm by Pseudomonas aeruginosa (PA) is one of the hallmarks of their survival both in vivo and in harsh environmental conditions, thus, biofilm-eradication has relevance from therapeutic perspectives and for infection control. The aim of our study was to investigate the possible relationship between antibiotic resistance, biofilm-forming capacity and virulence factors in n = 166 PA isolates of environmental origin. Antimicrobial susceptibility testing and the phenotypic detection of resistance determinants were carried out using standard protocols. The biofilm-forming capacity of PA was tested using a standardized crystal violet microtiter plate-based method. Motility (swimming, swarming, and twitching) and siderophore production of the isolates were also assessed. Resistance rates were highest for ciprofloxacin (46.98%), levofloxacin (45.18%), ceftazidime (31.92%) and cefepime (30.12%); 19.28% of isolates met the criteria to be classified as multidrug-resistant (MDR). Efflux pump overexpression, AmpC overexpression, and modified Hodge-test positivity were noted in 28.31%, 18.07% and 3.61%, respectively. 22.89% of isolates were weak/non-biofilm producers, while 27.71% and 49.40% were moderate and strong biofilm producers, respectively. Based on MDR status of the isolates, no significant differences in biofilm-production were shown among environmental PA (non-MDR OD570 [mean ± SD]: 0.416 ± 0.167 vs. MDR OD570: 0.399 ± 0.192; p > 0.05). No significant association was observed between either motility types in the context of drug resistance or biofilm-forming capacity (p > 0.05). 83.13% of isolates tested were positive for siderophore production. The importance of PA as a pathogen in chronic and healthcare-associated infections has been described extensively, while there is increasing awareness of PA as an environmental agent in agriculture and aquaculture. Additional studies in this field would be an important undertaking to understand the interrelated nature of biofilm production and antimicrobial resistance, as these insights may become relevant bases for developing novel therapeutics and eradication strategies against PA.

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“…These data demonstrate that Pa secretes active substances able to kill Cryptococcus spp. T3SS is a host cell-dependent protein secretion pathway and stands out for the secretion of two exotoxins of great invasive power on eukaryotic cells: ExoS and ExoU ( Yousefi-Avarvand et al., 2015 ; Radó et al., 2017 ). However, even in the absence of these exotoxins, using the strains with deletion for the secretion of ExoS (PAKΔexoS) and ExoU (PA103ΔexoU), other metabolites secreted by these mutants inhibited the growth of C. gattii and C. neoformans ( Figure 1G ).…”

Section: Discussionmentioning

confidence: 99%

Pseudomonas aeruginosa Infection Modulates the Immune Response and Increases Mice Resistance to Cryptococcus gattii

Peres-Emidio

Freitas

Costa

et al. 2022

Front. Cell. Infect. Microbiol.

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Cryptococcosis is an invasive mycosis caused by Cryptococcus spp. that affects the lungs and the central nervous system (CNS). Due to the severity of the disease, it may occur concomitantly with other pathogens, as a coinfection. Pseudomonas aeruginosa (Pa), an opportunistic pathogen, can also cause pneumonia. In this work, we studied the interaction of C. gattii (Cg) and Pa, both in vitro and in vivo. Pa reduced growth of Cg by the secretion of inhibitory molecules in vitro. Macrophages previously stimulated with Pa presented increased fungicidal activity. In vivo, previous Pa infection reduced morbidity and delayed the lethality due to cryptococcosis. This phenotype was correlated with the decreased fungal burden in the lungs and brain, showing a delay of Cg translocation to the CNS. Also, there was increased production of IL-1β, CXCL-1, and IL-10, together with the influx of iNOS-positive macrophages and neutrophils to the lungs. Altogether, Pa turned the lung into a hostile environment to the growth of a secondary pathogen, making it difficult for the fungus to translocate to the CNS. Further, iNOS inhibition reverted the Pa protective phenotype, suggesting its important role in the coinfection. Altogether, the primary Pa infection leads to balanced pro-inflammatory and anti-inflammatory responses during Cg infection. This response provided better control of cryptococcosis and was decisive for the mild evolution of the disease and prolonged survival of coinfected mice in a mechanism dependent on iNOS.

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Characterization of environmental Pseudomonas aeruginosa using multilocus sequence typing scheme

Cited by 20 publications

References 57 publications

Nitrofurantoin—Microbial Degradation and Interactions with Environmental Bacterial Strains

Nitrofurantoin—Microbial Degradation and Interactions with Environmental Bacterial Strains

Relationship between Biofilm-Formation, Phenotypic Virulence Factors and Antibiotic Resistance in Environmental Pseudomonas aeruginosa

Pseudomonas aeruginosa Infection Modulates the Immune Response and Increases Mice Resistance to Cryptococcus gattii

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