Characterization of environmental isolates of Enterococcus spp. by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

Giebel, Rebecca A.; Fredenberg, Weston; Sandrin, Todd R.

doi:10.1016/j.watres.2007.09.005

Cited by 58 publications

(38 citation statements)

References 32 publications

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“…MALDI-TOF MS has been used for the characterization of enterococcal isolates from both human and environmental sources (122,123). Böhme et al documented the accuracy of the Bruker BioTyper system for identification of 30 Enterococcus spp.…”

Section: Enterococcus Sppmentioning

confidence: 99%

“…A second study utilized MALDI-TOF MS as a bacterial source-tracking tool to characterize environmental isolates of Enterococcus spp. Animal and human enterococcal isolates were collected and analyzed to determine if MS spectra could be used to group isolates according to their respective sources (123). MALDI-TOF MS showed promise as a source-tracking tool for environmental studies, but the technique required further refinement, particularly in the sample-processing phase of experimentation.…”

Section: Enterococcus Sppmentioning

confidence: 99%

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Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry: a Fundamental Shift in the Routine Practice of Clinical Microbiology

et al. 2013

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SUMMARY Within the past decade, clinical microbiology laboratories experienced revolutionary changes in the way in which microorganisms are identified, moving away from slow, traditional microbial identification algorithms toward rapid molecular methods and mass spectrometry (MS). Historically, MS was clinically utilized as a high-complexity method adapted for protein-centered analysis of samples in chemistry and hematology laboratories. Today, matrix-assisted laser desorption ionization–time of flight (MALDI-TOF) MS is adapted for use in microbiology laboratories, where it serves as a paradigm-shifting, rapid, and robust method for accurate microbial identification. Multiple instrument platforms, marketed by well-established manufacturers, are beginning to displace automated phenotypic identification instruments and in some cases genetic sequence-based identification practices. This review summarizes the current position of MALDI-TOF MS in clinical research and in diagnostic clinical microbiology laboratories and serves as a primer to examine the “nuts and bolts” of MALDI-TOF MS, highlighting research associated with sample preparation, spectral analysis, and accuracy. Currently available MALDI-TOF MS hardware and software platforms that support the use of MALDI-TOF with direct and precultured specimens and integration of the technology into the laboratory workflow are also discussed. Finally, this review closes with a prospective view of the future of MALDI-TOF MS in the clinical microbiology laboratory to accelerate diagnosis and microbial identification to improve patient care.

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Section: Enterococcus Sppmentioning

confidence: 99%

Section: Enterococcus Sppmentioning

confidence: 99%

Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry: a Fundamental Shift in the Routine Practice of Clinical Microbiology

et al. 2013

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“…The influence of lysozyme treatment on the Enterococcus spp. MALDI mass spectra was studied in detail by Giebel, Fredenberg, and Sandrin (2008). The best repeatability of the obtained MALDI-MS profile was achieved after 30 min of exposure to lysozyme.…”

Section: Lysozyme Applicationmentioning

confidence: 99%

Sample preparation methods for MALDI‐MS profiling of bacteria

Šedo¹,

Sedláček²,

Zdráhal³

2010

Mass Spectrometry Reviews

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Direct matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) bacterial cell or lysate analysis appears to meet all the criteria required for a rapid and reliable analytical microorganism identification and taxonomical classification tool. Few-minute analytical procedure providing information extending up to sub-species level underlines the potential of the MALDI-MS profiling in comparison with other methods employed in the field. However, the quality of MALDI-MS profiles and consequently the performance of the method are influenced by numerous factors, which involve particular steps of the sample preparation procedure. This review is aimed at advances in development and optimization of the MALDI-MS profiling methodology. Approaches improving the quality of the MALDI-MS profiles and universal feasibility of the method are discussed.

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“…atrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has been applied in medical (1), environmental (2)(3)(4), and food-related (5-8) studies as an excellent cost-and time-effective tool for the identification of microorganisms. Different types of identification approaches were recently reviewed (9).…”

mentioning

confidence: 99%

Effects of Growth Medium on Matrix-Assisted Laser Desorption–Ionization Time of Flight Mass Spectra: a Case Study of Acetic Acid Bacteria

Wieme

Spitaels

Aerts

et al. 2014

Appl Environ Microbiol

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The effect of the growth medium used on the matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectra generated and its consequences for species and strain level differentiation of acetic acid bacteria (AAB) were determined by using a set of 25 strains. The strains were grown on five different culture media that yielded a total of more than 600 mass spectra, including technical and biological replicates. The results demonstrate that the culture medium can have a profound effect on the mass spectra of AAB as observed in the presence and varying signal intensities of peak classes, in particular when culture media do not sustain optimal growth. The observed growth medium effects do not disturb species level differentiation but strongly affect the potential for strain level differentiation. The data prove that a well-constructed and robust MALDI-TOF mass spectrometry identification database should comprise mass spectra of multiple reference strains per species grown on different culture media to facilitate species and strain level differentiation. Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has been applied in medical (1), environmental (2-4), and food-related (5-8) studies as an excellent cost-and time-effective tool for the identification of microorganisms. Different types of identification approaches were recently reviewed (9). These include linking of protein biomarkers derived from the mass spectra to a corresponding experimental database or comparing mass spectra with in silico-generated spectra predicted by whole-genome sequence analyses (10, 11). Yet, the most frequently used approach is the comparison of mass spectra of unknowns with those of well-characterized reference strains stored in a profile database. These comparisons are conducted by using either a peak-or a curve-based algorithm (9, 12). With a peak-based algorithm, the presence of specific biomarker peaks in the unknown isolate's mass spectrum is verified (6, 13). A curve-based method considers the complete spectrum, i.e., not only the presence of certain peaks but also the variation in peak signal intensity. The major drawback of these pattern recognition approaches is that they can be prone to experimental variations (14).Previous studies revealed that MALDI-TOF mass spectra of bacteria consist of signals derived mostly from ribosomal and other abundant proteins (15-18). For proteins that are of ribosomal origin (19), the effect of growth conditions on the mass spectra and thus on the identification result is expected to be minimal (20). However, growth conditions can influence the expression pattern of other proteins (21) and hence alter the mass spectrum (16,22). Additionally, growth medium compounds can interfere with the ionization of the bacterial biomolecules, especially when the bacterial cells have a tendency to adhere to the culture medium surface (15). Nevertheless, several studies found that variations related to the growth medium had no impact on the species l...

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Characterization of environmental isolates of Enterococcus spp. by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

Cited by 58 publications

References 32 publications

Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry: a Fundamental Shift in the Routine Practice of Clinical Microbiology

Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry: a Fundamental Shift in the Routine Practice of Clinical Microbiology

Sample preparation methods for MALDI‐MS profiling of bacteria

Effects of Growth Medium on Matrix-Assisted Laser Desorption–Ionization Time of Flight Mass Spectra: a Case Study of Acetic Acid Bacteria

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