Characterization of Enterococcus faecium EO1 isolated from mutton and activity of bacteriocin-like substances in the control of Listeria monocytogenes in fresh mutton sausage

Costa, Roger Junges da; Silva, Andresa Pereira da; Fonseca, Renata Nobre da; Hübner, Sílvia de Oliveira; Nalério, Élen Silveira; Marques, Juliana de Lima; Vitola, Helena Reissig Soares; Silva, Wladimir Padilha da; Duval, Eduarda Hallal; Fiorentini, Ângela Maria

doi:10.1016/j.lwt.2021.110954

Cited by 8 publications

(1 citation statement)

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“…Enterococcal strains can produce bacteriocins, and several of these probiotic proteins have been purified and genetically characterized to control pathogenic bacteria [ 8 ]. Despite their benefits, certain enterococcal strains are associated with their pathogenic characteristic as opportunistic bacteria.…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization of tetracycline and vancomycin-resistant Enterococcus faecium isolates from healthy dogs in Egypt: a public health threat

El-Razik,

Ibrahim,

Arafa

et al. 2023

BMC Genomics

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Background Vancomycin-resistant enterococci (VRE) are among the most common causative pathogens for nosocomial infections worldwide. Moreover, strains of VRE have been isolated from several domestic livestock in Egypt. Methods This study examined if healthy dogs are a potential source of VRE infection by isolating and characterizing Enterococcus faecium strains from stool samples on a morphological basis and biochemical activities. Subsequently, it was confirmed by genotypic characterization using polymerase chain reaction (PCR), followed by the detection of antibiotic resistance genes, virulence determinants, and genes contributing to enterocin production by PCR. Furthermore, the phylogenetic relationships among vanB and tetL genes were analyzed. Results All ten fecal samples were identified as E. faecium and confirmed by PCR. In addition, 90% of the isolates tested were positive for the virulence genes gelE and esp, and all the isolates tested were positive for the antibiotic resistance genes tetL and vanB. Only three of the five enterocin genes examined were detected. Ent As-48, bacteriocin 31, and Ent L50 were identified in 100%, 80%, and 60% of the samples, respectively. Conclusion Dogs should be regarded as a reservoir of E. faecium that carries vancomycin resistance and virulence determinants that may affect public health in Egypt, considering a "One Health" task force approach to restrict their spread.

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Section: Introductionmentioning

confidence: 99%