Characterization of Enterobius vermicularis in a human population, employing a molecular-based method from adhesive tape samples

Piperaki, Evangelia-Theophano; Spanakos, Gregory; Patsantara, Giannoula; Vassalou, Evdokia; Vakalis, Nikolaos; Tsakris, Athanassios

doi:10.1016/j.mcp.2011.03.005

Cited by 18 publications

(27 citation statements)

References 19 publications

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“…On the other hand, just like in Danish and Greek populations, a new haplotype, unregistered in GenBank, was detected. The E. vermicularis haplotypes revealed in hereby studies in Poland, similarly to the haplotypes from humans in Greece, Denmark and Germany (Piperaki et al, 2011;Ferrero et al, 2013) clustered within type B, together with the Japanese haplotypes from captive chimpanzees and humans. Type B is the only genetic type of this parasite which has so far been identifi ed in Europe.…”

Section: Discussionsupporting

confidence: 60%

“…Females from positive slides were excised with a piece of tape to extract DNA. A universal kit for DNA isolation from biological traces, Sherlock AX (A&A Biotechnology, Poland) was used according to the manufacturer's instructions with modifi cation according to Piperaki et al (2011) Modifi cation was used in the fi rst step of DNA extraction from females identifi ed on perianal swabs. DNA was eluted in 50 μl of TE buffer.…”

Section: Dna Isolationmentioning

confidence: 99%

“…For the PCR primers EVM1/EVM2 (Piperaki et al, 2011) targeting the region of E. vermicularis mitochondrial cytochrome c oxidase subunit 1 (cox1) gene (accession no. EU281143) were used.…”

Section: Dna Amplifi Cationmentioning

confidence: 99%

“…DNA sequencing reactions were carried out in both directions. The sequences were aligned and trimmed to the length of 333 bp (primers excluded) according to the previously published cox1 alignments of E. vermicularis isolates (Nakano et al, 2006;Piperaki et al, 2011;Ferrero et al, 2013) available in the GenBank database. Phylogenetic analysis was performed using the neighbour-joining method in a Geneious v.7.0 computer program (Biomatters).…”

Section: Sequencing and Phylogenetic Analysismentioning

confidence: 99%

“…Despite that fact, E. vermicularis genetic variability and its epidemiology of based on molecular typing have not been fully investigated (Iñiguez et al,. 2006;Piperaki et al, 2011;Zelck et al, 2011;Ferrero et al, 2013). Complete data on the DNA sequence are only available in relation to the mitochondrial genome of E. vermicularis (Kang et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

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Enterobiasis epidemiology and molecular characterization of Enterobius vermicularis in healthy children in north-eastern Poland

2017

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SummaryEnterobiasis is a human intestinal parasitic disease caused by human pinworm, Enterobius vermicularis. Despite being the most prevalent nematode infection in Europe and North America, predominantly among in school aged children, the data concerning infection rate and knowledge of genetic variability of pinworms are incomplete. The aim of the study was the estimation of prevalence and molecular typing of Enterobius vermicularis among healthy children in north-eastern Poland. In 2013 -2015, 296 individuals (aged 2 -18 years) from 12 kindergartens, schools and orphanages were examined by the adhesive cellophane tape method. Data on socio-demographic status were collected using a questionnaire. Molecular analysis was performed using the DNA of adult female pinworms and primers targeting the region of cytochrome oxidase I gene. The overall prevalence of enterobiasis was 10.1 %. Enterobius vermicularis infection rates were 3.9 % in children living in families and 32.8 % among the orphans (OR=0.08; 95 % CI: 0.04 -0.19; p<0.001). There were no associations between distribution of enterobiasis and gender, pets possession and the season of examination. In 43.3 % of the infected children enterobiasis was asymptomatic. Based on a molecular marker three different haplotypes of pinworm were identifi ed. All sequences clustered within type B, together with human E. vermicularis isolates from Denmark, Germany, Greece, and Japan. This paper provides complementary data on the occurrence and intraspecifi c variability of E. vermicularis in human population in Europe.

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Section: Discussionsupporting

confidence: 60%

Section: Dna Isolationmentioning

confidence: 99%

“…For the PCR primers EVM1/EVM2 (Piperaki et al, 2011) targeting the region of E. vermicularis mitochondrial cytochrome c oxidase subunit 1 (cox1) gene (accession no. EU281143) were used.…”

Section: Dna Amplifi Cationmentioning

confidence: 99%

Section: Sequencing and Phylogenetic Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Enterobiasis epidemiology and molecular characterization of Enterobius vermicularis in healthy children in north-eastern Poland

2017

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Adhesive-tape recovery combined with molecular and microscopic testing for the detection of Cryptosporidium oocysts on experimentally contaminated fresh produce and a food preparation surface

Fayer¹,

Santı́n

Macarisin

et al. 2013

Parasitol Res

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A proof of concept study was conducted to determine if transparent double-sided adhesive tape could be used to recover and detect [by polymerase chain reaction (PCR) and immunofluorescence microscopy (IFA)] Cryptosporidium parvum oocysts on fresh produce and on a food preparation surface. Oocysts were applied on the surface of ten apples, ten peaches, eight cucumbers, and eight tomatoes within circles drawn with a permanent marker. Approximately 18 h later, skin excised from three uncontaminated and three contaminated circles from each piece of produce was subjected to PCR. Pieces of transparent double-sided adhesive tape were lightly pressed onto the surface of three other contaminated circles and examined by PCR. Other pieces of adhesive tape were pressed against the surfaces of three other circles and examined by IFA. At concentrations of 100 and 50 oocysts per circle, every produce item examined by PCR of contaminated excised skin was found positive, and every item examined by adhesive tape subjected to PCR and IFA was found positive, except one. At ten oocysts per circle, every produce item was found positive by PCR of contaminated excised skin, and all apples, cucumbers, and tomatoes were found positive by adhesive tape subjected to IFA. Detection of low numbers of oocysts on peaches by IFA examination of adhesive tape was problematic because trichomes that cover peaches and impart the fuzzy surface partially restrict the tape from reaching some areas where oocysts adhere. Tape combined with IFA was successful in recovering and identifying oocysts from six areas of laminate countertop where the oocysts had been applied and allowed to dry for 30-60 min. These are the first findings to demonstrate that adhesive tape can be used to recover and identify a protozoan parasite from fresh produce and from a laminate food preparation surface.

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Prevalence and genetic analysis of Enterobius vermicularis in schoolchildren in lower northern Thailand

Janthu

Dumidae²,

Subkrasae³

et al. 2022

Parasitol Res

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Characterization of Enterobius vermicularis in a human population, employing a molecular-based method from adhesive tape samples

Cited by 18 publications

References 19 publications

Enterobiasis epidemiology and molecular characterization of Enterobius vermicularis in healthy children in north-eastern Poland

Enterobiasis epidemiology and molecular characterization of Enterobius vermicularis in healthy children in north-eastern Poland

Adhesive-tape recovery combined with molecular and microscopic testing for the detection of Cryptosporidium oocysts on experimentally contaminated fresh produce and a food preparation surface

Prevalence and genetic analysis of Enterobius vermicularis in schoolchildren in lower northern Thailand

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