Characterization of Endophytic Microbial Communities in Store-Bought Kale Evaluated by Different Plant Tissue Homogenization Methods

McNees, C. Ruth; Law, Audrey D.; Moe, Luke A.

doi:10.1094/pbiomes-08-19-0046-sc

Cited by 3 publications

(6 citation statements)

References 38 publications

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“…Root samples of M. sinensis taken from the Xikuangshan (XKS) site were chosen to extract the cultivable endophytic microorganisms using a Nycodenz density-gradient centrifugation method [ 37 – 39 ]. To ensure only endophytes were extracted, the surface sterilization was further confirmed by applying wash buffer (R1, R2, R3) to LB agar plates.…”

Section: Methodsmentioning

confidence: 99%

Characterization of diazotrophic root endophytes in Chinese silvergrass (Miscanthus sinensis)

Yang

Häggblom

et al. 2022

Microbiome

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Background Phytoremediation is a potentially cost-effective way to remediate highly contaminated mine tailing sites. However, nutrient limitations, especially the deficiency of nitrogen (N), can hinder the growth of plants and impair the phytoremediation of mine tailings. Nevertheless, pioneer plants can successfully colonize mine tailings and exhibit potential for tailing phytoremediation. Diazotrophs, especially diazotrophic endophytes, can promote the growth of their host plants. This was tested in a mine-tailing habitat by a combination of field sampling, DNA-stable isotope probing (SIP) analysis, and pot experiments. Results Bacteria belonging to the genera Herbaspirillum, Rhizobium, Devosia, Pseudomonas, Microbacterium, and Delftia are crucial endophytes for Chinese silvergrass (Miscanthus sinensis) grown in the tailing, the model pioneer plant selected in this study. Further, DNA-SIP using 15N2 identified Pseudomonas, Rhizobium, and Exiguobacterium as putative diazotrophic endophytes of M. sinensis. Metagenomic-binning suggested that these bacteria contained essential genes for nitrogen fixation and plant growth promotion. Finally, two diazotrophic endophytes Rhizobium sp. G-14 and Pseudomonas sp. Y-5 were isolated from M. sinensis. Inoculation of another pioneer plant in mine tailings, Bidens pilosa, with diazotrophic endophytes resulted in successful plant colonization, significantly increased nitrogen fixation activity, and promotion of plant growth. Conclusions This study indicated that diazotrophic endophytes have the potential to promote the growth of pioneer plant B. pilosa in mine tailings.

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Section: Methodsmentioning

confidence: 99%

Characterization of diazotrophic root endophytes in Chinese silvergrass (Miscanthus sinensis)

Yang

Häggblom

et al. 2022

Microbiome

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“…Studies of the microbes colonizing leaves are also of great importance in the field of bacteriological safety in the food industry [ 63 , 70 ]. It has been shown, for example, that although the enteric pathogen Salmonella enterica, is not a native plant endophyte, can actively infiltrate the deep leaf structures and multiply there [ 47 ].…”

Section: Research Of Plant-microorganism Relationshipsmentioning

confidence: 99%

“…Properly collected AWF contains at most only traces of cell contamination, so it is worth considering the use of infiltration-centrifugation as an attractive alternative to invasive methods of plant tissue homogenization used to study microorganisms inhabiting internal leaf tissues [ 2 , 70 ]. It is worth considering the adaptation of AWF extraction procedures towards using them as a starting material for culture-independent microbiome studies using, for example the Illumina Miseq platform.…”

Section: Research Of Plant-microorganism Relationshipsmentioning

confidence: 99%

“…It is worth considering the adaptation of AWF extraction procedures towards using them as a starting material for culture-independent microbiome studies using, for example the Illumina Miseq platform. The material for metagenomic analyses obtained in this way could help to circumvent some of the basic problems encountered when using DNA derived from whole leaves macerated using standard protocols, including the low-throughput nature of plant tissue maceration and the prevalence of plant plastid DNA in metagenomic DNA extracts, which is typically coamplified via PCR strategies that target the bacterial 16S rRNA gene [ 70 ]. The method of pure AWF extraction for metagenomic DNA purification and library preparation could be helpful to overcome these problems.…”

Section: Research Of Plant-microorganism Relationshipsmentioning

confidence: 99%

“…Recently, plant tissues infiltration mechanisms are being studied not only by researchers whose aim is to improve infiltration effectivity but also in research areas where infiltration is perceived as a very unfavourable process, for example in the food industry dealing with microbial food contamination [ 14 , 20 , 70 ].…”

Section: Infiltration In the Food Industry Is Not Always A Desirable Phenomenonmentioning

confidence: 99%

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Leaf infiltration in plant science: old method, new possibilities

Chincinska

2021

Plant Methods

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The penetration of substances from the surface to deep inside plant tissues is called infiltration. Although various plant tissues may be effectively saturated with externally applied fluid, most described infiltration strategies have been developed for leaves. The infiltration process can be spontaneous (under normal atmospheric pressure) or forced by a pressure difference generated between the lamina surface and the inside of the leaf. Spontaneous infiltration of leaf laminae is possible with the use of liquids with sufficiently low surface tension. Forced infiltration is most commonly performed using needle-less syringes or vacuum pumps.Leaf infiltration is widely used in plant sciences for both research and application purposes, usually as a starting technique to obtain plant material for advanced experimental procedures. Leaf infiltration followed by gentle centrifugation allows to obtain the apoplastic fluid for further analyses including various omics. In studies of plant-microorganism interactions, infiltration is used for the controlled introduction of bacterial suspensions into leaf tissues or for the isolation of microorganisms inhabiting apoplastic spaces of leaves. The methods based on infiltration of target tissues allow the penetration of dyes, fixatives and other substances improving the quality of microscopic imaging. Infiltration has found a special application in plant biotechnology as a method of transient transformation with the use of Agrobacterium suspension (agroinfiltration) enabling genetic modifications of mature plant leaves, including the local induction of mutations using genome editing tools. In plant nanobiotechnology, the leaves of the target plants can be infiltrated with suitably prepared nanoparticles, which can act as light sensors or increase the plant resistance to environmental stress. In addition the infiltration has been also intensively studied due to the undesirable effects of this phenomenon in some food technology sectors, such as accidental contamination of leafy greens with pathogenic bacteria during the vacuum cooling process.This review, inspired by the growing interest of the scientists from various fields of plant science in the phenomenon of infiltration, provides the description of different infiltration methods and summarizes the recent applications of this technique in plant physiology, phytopathology and plant (nano-)biotechnology.

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Pathogenic fungi shape the fungal community, network complexity, and pathogenesis in kiwifruit

Huang,

Sun,

et al. 2023

Microbial Biotechnology

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Kiwifruit decay caused by endophytic fungi is affected by exogenous pathogens that trigger changes in fungal community composition and interact with the endophytic fungal community. Four fungal pathogens of kiwifruit were identified. These were Aspergillus japonicus, Aspergillus flavus, Botryosphaeria dothidea, and Penicillium oxalicum. Except for P. oxalicum, the remaining three species represent newly described pathogens of kiwifruit. All four fungal species caused disease and decay in mature kiwifruit. Results of the fungal community analysis indicated that three pathogens that A. japonicus, A. flavus and P. oxalicum were the most dominant, however, other fungal species that did not cause disease symptoms were also present. Positive interactions between fungal species were found in asymptomatic, symptomatic, and infected kiwifruit. The ability of all four pathogens to infect kiwifruit was confirmed in an inoculation experiment. The presence of any one of the four identified pathogens accelerated decay development and limited the postharvest longevity of harvested kiwifruit. Results of the study identified and confirmed the ability of four fungal species to infect and cause decay in harvested kiwifruit. Changes in the structure and composition of the kiwifruit microbiome during the decay process were also characterized. This provides a foundation for the further study of the microbiome of kiwifruit and their involvement in postharvest diseases.

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Characterization of Endophytic Microbial Communities in Store-Bought Kale Evaluated by Different Plant Tissue Homogenization Methods

Cited by 3 publications

References 38 publications

Characterization of diazotrophic root endophytes in Chinese silvergrass (Miscanthus sinensis)

Characterization of diazotrophic root endophytes in Chinese silvergrass (Miscanthus sinensis)

Leaf infiltration in plant science: old method, new possibilities

Pathogenic fungi shape the fungal community, network complexity, and pathogenesis in kiwifruit

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