Characterization of Endophytic Bacteria With Plant Growth Promoting Activities Isolated From Six Medicinal Plants

Abstract

The present investigation aimed to isolate and characterize endophytic bacteria from six selected medicinal plants (Ocimum sanctum, Aegle marmelos, Cinnamomum cassia, Azadirachta indica, Calotropis procera and Rauwolfia serpentina) with plant growth promoting activities. Total 38 endophytic bacteria were isolated from the studied plant species by following standard microbiological culture methods. All the isolated bacteria were screened for the production of hydrolyzing enzyme and result of study revealed that maximum isolates have positive catalytic activity and this was followed by amylase, lipase and protease activities. While studying the antibacterial activity of the isolates, it was observed that fifteen isolates showed moderate to higher antibacterial activities against Escherichia coli, Pseudomonas aeruginosa & Staphylococcus aureus. Studies on plant growth promoting activity suggested that 8, 12 & 6 isolates were positive for IAA production, siderophore production and phosphate solubilization activity respectively. Further, eight (21.05%) isolates were found positive for ammonia production. Through this investigation, endophytic bacteria isolated from medicinal plants with plant growth promoting activities were reported, that can be further exploited for sustainable agriculture, with detail scientific investigation.

Isolation and plant growth promoting properties of bacterial endophytes from wild plant (Wedelia urticifolia) of south Gujarat region

“…The phenomenon of solubilization of potassium from soil by endophytic microbes mostly dependent on the pH and type of soil [18] . Endophytic bacteria belonging to genera Achromobacter, Acinetobacter, Bacillus, Burkholderia [19,20,22,32] have been reported for potassium-solubilization. In the present study, three among fourteen isolates were able to solubilize potassium by showing zone of solubilization.…”

“…In the present study, three among fourteen isolates were able to solubilize potassium by showing zone of solubilization. In previous studies, Bacillus licheniformis and Pseudomonas azotoformans which were isolated from the soil of rice plants, using two potassium solubilizing bacteria, could increase the availability of potassium in the soil [20] . Another study concluded that among all investigated Actinobacteria strains; Streptomyces alboviridis, Streptomyces griseorubens and Nocardiopsis Alba were able to solubilize potassium [22] .…”

“…To produce 1 L of siderophore media, the glassware were defer rated by washing with 6-M HCl overnight to eliminate ~ 43 ~ the residual Fe contamination, then rinsed with double distilled water two to three times [15,20] . To measure siderophores, the CAS (Chrome Azurol S) plate method was employed [21] .…”

Characterization of a yellow pigmented, alkali and heavy metal tolerant Glutamicibacter sp. isolated from red mud

“…Antimicrobial activity of the pigment was studied following Panigrahi et al, 2018. Six pathogens namely, Escherichiacoli MTCC 723, Pseudomon asaureus MTCC 741, Vibrio cholerae MTCC 3906, Staphylococcus aureus MTCC 902, Klebsiella pneumoniae MTCC109 and Enterococcus cloacae MTCC 7322 were used.…”

Comprehensive assessment of growth parameters for screening endophytic bacterial strains in Solanum lycopersicum (Tomato)

“…Considering this, an array of sustainable agricultural practices involving soil microbiota and endophytes are being suggested for a stable enhancement in plant health and productivity (Lucy et al, 2004;Arcand and Schneider, 2006). Among all the classes of microbes which have beneficial interactions with plant, the endophytes have gained special interest (Kobayashi and Palumbo, 2000;Dudeja et al, 2012;Panigrahi et al, 2018) as these microorganisms are not only critical for promoting plant growth and productivity (Kloepper and Schroth, 1981), but having a specialized niche within the plant tissues, they act as biocontrol agents (Mercado--Blanco and Bakker, 2007;Ryan et al, 2008). The colonizing capabilities and persistency of these microbes determines their resilience and diversity within the plant and in nature respectively (Rosenblueth and Martínez-Romero, 2006).…”