Characterization of endophytic bacteria Enterobacter cloacae MG00145 isolated from Ocimum sanctum with Indole Acetic Acid (IAA) production and plant growth promoting capabilities against selected crops

Panigrahi, S.; Mohanty, Santosh Ranjan; Rath, Chandi Charan

doi:10.1016/j.sajb.2019.09.017

Cited by 51 publications

(35 citation statements)

References 35 publications

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“…cloacae ATCC 13047 [ 31 ] which confirms the presence of multiple pathways for IAA biosynthesis in these bacteria. Indole 3-pyruvic acid pathway in E. cloacae was reported in previous studies [ 20 , 32 , 33 ]. Interestingly, the extract Zch127 showed higher antivirulence activity against tested isolates compared to Cbg70 which could be attributed to the presence of the three unique compounds IAN, methyl indole and I3CA.…”

Section: Resultssupporting

confidence: 60%

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Indole Derivatives Obtained from Egyptian Enterobacter sp. Soil Isolates Exhibit Antivirulence Activities against Uropathogenic Proteus mirabilis

et al. 2021

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Proteus mirabilis is a frequent cause of catheter associated urinary tract infections (CAUTIs). Several virulence factors contribute to its pathogenesis, but swarming motility, biofilm formation, and urease activity are considered the hallmarks. The increased prevalence in antibiotic resistance among uropathogens is alarming and requires searching for new treatment alternatives. With this in mind, our study aims to investigate antivirulence activity of indole derivatives against multidrug resistant P. mirabilis isolates. Ethyl acetate (EtOAc) extracts from Enterobacter sp. (rhizobacterium), isolated from Egyptian soil samples were tested for their ability to antagonize the virulence capacity and biofilm activity of P. mirabilis uropathogens. Extracts of two Enterobacter sp. isolates (coded Zch127 and Cbg70) showed the highest antivirulence activities against P. mirabilis. The two promising rhizobacteria Zch127 and Cbg70 were isolated from soil surrounding: Cucurbita pepo (Zucchini) and Brassica oleracea var. capitata L. (Cabbage), respectively. Sub-minimum inhibitory concentrations (Sub-MICs) of the two extracts showed potent antibiofilm activity with significant biofilm reduction of ten P. mirabilis clinical isolates (p-value < 0.05) in a dose-dependent manner. Interestingly, the Zch127 extract showed anti-urease, anti-swarming and anti-swimming activity against the tested strains. Indole derivatives identified represented key components of indole pyruvate, indole acetamide pathways; involved in the synthesis of indole acetic acid. Additional compounds for indole acetonitrile pathway were detected in the Zch127 extract which showed higher antivirulence activity. Accordingly, the findings of the current study model the feasibility of using these extracts as promising antivirulence agent against the P. mirabilis uropathogens and as potential therapy for treatment of urinary tract infections (UTIs).

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Section: Resultssupporting

confidence: 60%

“…Enterobacter sp. has been previously shown to be abundant in the soil samples [ 18 , 19 , 20 ]. The phylogenetic tree of the two selected strains and highly similar strains in the NCBI database was constructed using Mega 6.0 ( Figure S1 ).…”

Section: Resultsmentioning

confidence: 99%

Indole Derivatives Obtained from Egyptian Enterobacter sp. Soil Isolates Exhibit Antivirulence Activities against Uropathogenic Proteus mirabilis

et al. 2021

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“…Enterobacter cloacae is another popular example of PGP Enterobacter which is ubiquitous in soil, sewage, water, and food. The efficiency of endophytic E. cloacae's application on crop productivity has been discussed extensively in the previous reports which encompassed its functional phenotypic, genotypic and metabolomic aspects of the species [6,[13][14][15]. Interestingly, the PGP potential of its closed counterpart, Enterobacter hormaechei has been recently explored.…”

Section: Introductionmentioning

confidence: 99%

Seed biopriming with P- and K-solubilizing Enterobacter hormaechei sp. improves the early vegetative growth and the P and K uptake of okra (Abelmoschus esculentus) seedling

et al. 2020

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Limited information is available that seed biopriming with plant growth-promoting Enterobacter spp. play a prominent role to enhance vegetative growth of plants. Contrary to Enterobacter cloacae, Enterobacter hormaechei is a less-studied counterpart despite its vast potential in plant growth-promotion mainly through the inorganic phosphorus (P) and potassium (K) solubilization abilities. To this end, 18 locally isolated bacterial pure cultures were screened and three strains showed high P-and K-solubilizing capabilities. Light microscopy, biochemical tests and 16S rRNA gene sequencing revealed that strains 15a1 and 40a were closely related to Enterobacter hormaechei while strain 38 was closely related to Enterobacter cloacae (Accession number: MN294583; MN294585; MN294584). All Enterobacter spp. shared common plant growth-promoting traits, namely nitrogen (N 2) fixation, indole-3-acetic acid production and siderophore production. The strains 38 and 40a were able to produce gibberellic acid, while only strain 38 was able to secrete exopolysaccharide on agar. Under in vitro germination assay of okra (Abelmoschus esculentus) seeds, Enterobacter spp. significantly improved overall germination parameters and vigor index (19.6%) of seedlings. The efficacy of root colonization of Enterobacter spp. on the pre-treated seedling root tips was confirmed using Scanning Electron Microscopy (SEM). The pot experiment of bioprimed seeds of okra seedling showed significant improvement of the plant growth (> 28%) which corresponded to the increase of P and K uptakes (> 89%) as compared to the uninoculated control plants. The leaf surface area and the SPAD chlorophyll index of bioprimed plants were increased by up to 29% and 9% respectively. This report revealed that the under-explored species of P-and K-solubilizing Enterobacter hormaechei sp. with multiple plant beneficial traits presents a great potential sustainable approach for enhancement of soil fertility and P and K uptakes of plants.

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“…L-Asparaginase producing a bacterial endophyte has been reported from Ocimum tenuiflorum (Tulasi) which was identified as Bacillus stratosphericus by using 16 s rRNA sequencing [ 114 ]. Panigrahi et al [ 115 ] obtained Enterobacter cloacae , a bacterial endophyte from the stem of Ocimum sanctum . Sahu et al [ 116 ] in their study reported twenty-one endophytic bacteria recovered from Ocimum tenuiflorum .…”

Section: Endomicrobial Diversity Of Important Medicinal Plants/genusmentioning

confidence: 99%

“… Enterobacter cloacae MG00145 Alternaria tenuissima , Colletotricum gloesporioides , Diaporthe sp., and Trichoderma sp. - Anti-diabetic Singh et al [ 148 ]; Upadhyay et al [ 204 ]; Kumar et al [ 49 ]; Panigrahi et al [ 115 ] Roots and shoots Bacillus cereus Lasiodiplodia pseudotheobromae - Antibacterial activity and antimicrobial activity Nayak and Shashirekha [ 149 ]; Tanvir et al [ 150 ]; Taufiq and Darah [ 111 ] Roots - Paecilomyces variotti - Hepatoprotective and antioxidant activities Shukla et al [ 112 ] Ocimum basilicum var. Thyrsiflora Leaves To cure respiratory problem.…”

Section: Endomicrobial Diversity Of Important Medicinal Plants/genusmentioning

confidence: 99%

Bioprospecting of endophytes in medicinal plants of Thar Desert: An attractive resource for biopharmaceuticals

Yadav

Meena

2021

Biotechnology Reports

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Highlights Endophytes act as chemical synthesizers of the secondary metabolites of the host plant. Endophytic microflora has been isolated from a wide array of plants. It can serve as a good alternative source for pharmaceutically important compounds. The arid ecosystem of Thar Desert is a rich repository of ethnomedicinal plants. Role of endophytes in the synthesis of bioactive molecules used to enhance production.

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Characterization of endophytic bacteria Enterobacter cloacae MG00145 isolated from Ocimum sanctum with Indole Acetic Acid (IAA) production and plant growth promoting capabilities against selected crops

Cited by 51 publications

References 35 publications

Indole Derivatives Obtained from Egyptian Enterobacter sp. Soil Isolates Exhibit Antivirulence Activities against Uropathogenic Proteus mirabilis

Indole Derivatives Obtained from Egyptian Enterobacter sp. Soil Isolates Exhibit Antivirulence Activities against Uropathogenic Proteus mirabilis

Seed biopriming with P- and K-solubilizing Enterobacter hormaechei sp. improves the early vegetative growth and the P and K uptake of okra (Abelmoschus esculentus) seedling

Bioprospecting of endophytes in medicinal plants of Thar Desert: An attractive resource for biopharmaceuticals

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