Characterization of eleven monosomic alien addition lines added from Gossypium anomalum to Gossypium hirsutum using improved GISH and SSR markers

Abstract

Background Gossypium anomalum (BB genome) possesses the desirable characteristics of drought tolerance, resistance to diseases and insect pests, and the potential for high quality fibers. However, it is difficult to transfer the genes associated with these desirable traits into cultivated cotton (G. hirsutum, AADD genome). Monosomic alien addition lines (MAALs) can be used as a bridge to transfer desired genes from wild species into G. hirsutum. In cotton, however, the high number and smaller size of the chromosomes has resulted in difficulties in discriminating chromosomes from wild species in cultivated cotton background, the development of cotton MAALs has lagged far behind many other crops. To date, no set of G. hirsutum-G. anomalum MAALs was reported. Here the amphiploid (AADDBB genome) derived from G. hirsutum × G. anomalum was used to generate a set of G. hirsutum-G. anomalum MAALs through a combination of consecutive backcrossing, genomic in situ hybridization (GISH), morphological survey and microsatellite marker identification.ResultsWe improved the GISH technique used in our previous research by using a mixture of two probes from G. anomalum and G. herbaceum (AA genome). The results indicate that a ratio of 4:3 (G. anomalum : G. herbaceum) is the most suitable for discrimination of chromosomes from G. anomalum and the At-subgenome of G. hirsutum. Using this improved GISH technique, 108 MAAL individuals were isolated. Next, 170 G. hirsutum- and G. anomalum-specific codominant markers were obtained and employed for characterization of these MAAL individuals. Finally, eleven out of 13 MAALs were identified. Unfortunately, we were unable to isolate Chrs. 1Ba and 5Ba due to their very low incidences in backcrossing generation, as these remained in a condition of multiple additions.ConclusionsThe characterized lines can be employed as bridges for the transfer of desired genes from G. anomalum into G. hirsutum, as well as for gene assignment, isolation of chromosome-specific probes, development of chromosome-specific “paints” for fluorochrome-labeled DNA fragments, physical mapping, and selective isolation and mapping of cDNAs/genes for a particular G. anomalum chromosome.Electronic supplementary materialThe online version of this article (doi:10.1186/s12870-016-0913-2) contains supplementary material, which is available to authorized users.

A complete set of monosomic alien addition lines developed from <i>Gossypium anomalum</i> in a <i>Gossypium hirsutum</i> background: genotypic and phenotypic characterization

“…To date, there has been no report of the construction of a complete set of MAALs for diploid and tetraploid populations of Gossypium. In a previous study, MAALs covering Developing monosomic alien addition lines from G. anomalum Breeding Science Preview BS 11 G. anomalum chromosomes were developed in the cotton genotype TM-1 background (Wang et al 2016). In the present study, we developed a complete set of MAALs from G. anomalum chromosomes in the background of the cotton variety Su8289, which comprehensively shows good performance and makes a suitable recipient.…”

“…MAALs in the genetic background of Upland cotton have been obtained from a number of diploid Gossypium species such as G. anomalum ( Wang et al 2016 ), G. sturtianum (CC) ( Rooney et al 1991 ), G. somalense (E 2 E 2 ) ( Zhou et al 2004 ) and G. australe (G 2 G 2 ) ( Sarr et al 2011 ). It is, however, difficult to construct a whole set of diploid cotton MAALs due to the instability of alien chromosomes and time-consuming identification processes.…”

“… Chen et al (2014) developed a complete set of alien addition lines from G. australe in G. hirsutum , but two lines were not monosomic. Wang et al (2016) described 11 MAALs derived from G. anomalum into G. hirsutum , but MAALs of chromosome 1B and 5B were not obtained. To date, no complete set of MAALs from diploid cotton in Upland cotton has been reported.…”

Inducement and identification of chromosome introgression and translocation of Gossypium australe on Gossypium hirsutum

“…In addition, it is necessary to point out that until now, only two sets of chromosome alien addition lines in cotton had been developed by our lab [ 5 , 27 ]. Here, from the M 1 generation, we also found that 44 individuals were monosomic alien additions that involved 12 chromosomes (all except chromosome 13G).…”

Alien introgression and morpho-agronomic characterization of diploid progenies of Solanum lycopersicoides monosomic alien addition lines (MAALs) toward pre-breeding applications in tomato (S. lycopersicum)

“…MAALs, on the other hand, are pre-breeding lines that carry an additional chromosome from the wild donor alongside the full chromosome complement of the cultivated, recipient parent (2 n + 1) (Khush 2010 ). This distinctive feature of MAALs makes them highly suitable for assigning alien traits to specific chromosomes of the wild donor, dissecting wild genomes into individual chromosome entities in a functional genomic background, and identifying novel genes underlying traits of agronomic importance (Khush 2010 ; Wang et al 2016 ; Narain et al 2016 ). For introgressive breeding applications, however, the utilization of MAALs is largely restricted by the unstable transmission of the additional chromosome, as well as the potential loss of chromosome integrity due to homeologous recombinations (Miller and Tanksley 1990 ; Chetelat et al 1998 ).…”