1998
DOI: 10.1128/jvi.72.4.3155-3160.1998
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Characterization of Ebola Virus Entry by Using Pseudotyped Viruses: Identification of Receptor-Deficient Cell Lines

Abstract: Studies analyzing Ebola virus replication have been severely hampered by the extreme pathogenicity of this virus. To permit analysis of the host range and function of the Ebola virus glycoprotein (Ebo-GP), we have developed a system for pseudotyping these glycoproteins into murine leukemia virus (MLV). This pseudotyped virus, MLV(Ebola), can be readily concentrated to titers which exceed 5 × 106 infectious units/ml and is effectively neutralized by antibodies specific for Ebo-GP. Analysis of MLV(Ebola) infecti… Show more

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Cited by 336 publications
(178 citation statements)
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“…Pseudotyped viruses with heterogenic glycoproteins incorporated into retroviral particles were proved to be a safe viral entry model, which only go through a single-cycle infection and acquired the host range of the parent viruses where the glycoproteins were derived [4][5][6], thus they could facilitate the research on viral entry mechanism, cell tropism, neutralization antibody analysis, and receptor identification [7][8][9][10].…”
mentioning
confidence: 99%
“…Pseudotyped viruses with heterogenic glycoproteins incorporated into retroviral particles were proved to be a safe viral entry model, which only go through a single-cycle infection and acquired the host range of the parent viruses where the glycoproteins were derived [4][5][6], thus they could facilitate the research on viral entry mechanism, cell tropism, neutralization antibody analysis, and receptor identification [7][8][9][10].…”
mentioning
confidence: 99%
“…Data are means of duplicate wells of one representative experiment and the error bars represent the standard errors of the means. Matsuura et al, 2001;Moore et al, 2004;Nie et al, 2004;Simmons et al, 2004;Wool-Lewis and Bates, 1998). In this study, we have successfully established a retrovirus-based pseudotype reporter virus for four serotypes of dengue viruses.…”
Section: Discussionmentioning
confidence: 99%
“…Like other flaviviruses, dengue virus is believed to enter cells through receptor-mediated endocytosis, a process sensitive to low pH environment in the endosome (Guirakhoo et al, 1993;Lindenbach and Rice, 2001;Mellman et al, 1986;Mukhopadhyay et al, 2005;. To investigate entry of the pseudotype reporter virus, Huh7 cells were treated with increasing concentrations of two lysosomotropic agents, Bafilomycin A and ammonium chloride, and infected with D2(HIVluc) and VSV(HIVluc), which has been shown to enter cells through a pH-dependent route Nie et al, 2004;Simmons et al, 2004;Wool-Lewis and Bates, 1998). As shown in Fig.…”
Section: Ph-dependent Entry Of Pseudotype Reporter Virusmentioning
confidence: 99%
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“…It belongs to the genus Ebolavirus, within the family of Filoviridae. Its envelope glycoprotein facilitates the entry of the virus to living cells [7,8] . Till date five strains of the virus are known: Zaire, Sudan, Tai Forest, Bundibugyo, and Reston with Zaire stain being the most virulent with up to 90% fatalities [9].…”
Section: Introductionmentioning
confidence: 99%