Characterization of Ebinur Lake Virus and Its Human Seroprevalence at the China–Kazakhstan Border

Xia, Han; Liu, Ran; Zhao, Lu; Sun, Xiang Dong; Zheng, Zhong; Atoni, Evans; Hu, Xiaomin; Zhang, Bo; Zhang, Guilin; Yuan, Zhiming

doi:10.3389/fmicb.2019.03111

Cited by 20 publications

(36 citation statements)

References 35 publications

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“…In a previously conducted study by Pingen and team, they note that mosquito bites could significantly enhance infection with BUNV infection ( Pingen et al, 2016 ). Although EBIV has been isolated from Culex modestus ( Xia et al, 2020 ), the primary transmission vector of EBIV is still not clear. And we have not yet detected any EBIV RNA in the sampled Aedes flavipectus , Aedes Caspian , and Culex pipiens mosquitoes that formed part of our mosquito surveillance studies for the years 2014 and 2019 (data not shown).…”

Section: Discussionmentioning

confidence: 99%

“…The reported hosts of Bunyamwera serogroup viruses are rodents, sheep, cattle, equine, avian species, primates, or humans ( Dutuze et al, 2018 ). Being a not well-characterized virus, we do not know whether EBIV can cause disease to humans even though the seroprevalence clue of EBIV infection in humans has been reported ( Xia et al, 2020 ). It is possible that other vertebrate species, such as small mammals or birds could be more susceptible to EBIV.…”

Section: Discussionmentioning

confidence: 99%

“…BHK-21 was maintained at 37°C in Dulbecco’s minimal essential medium (DMEM; 4.5 g/liter D-glucose) containing 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin in 5% CO 2 . EBIV isolate Cu-XJ20 was first isolated from Culex modestus mosquitoes in Xinjiang, China ( Xia et al, 2020 ). The EBIV virus stock was propagated in BHK-21 cells in DMEM containing 2% FBS and stored in aliquots at −80°C.…”

Section: Methodsmentioning

confidence: 99%

“…Virus titrations were performed using BHK-21 cells as previously described ( Xia et al, 2020 ), and the results expressed as PFU ml −1 .…”

Section: Methodsmentioning

confidence: 99%

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Pathogenesis and Immune Response of Ebinur Lake Virus: A Newly Identified Orthobunyavirus That Exhibited Strong Virulence in Mice

et al. 2021

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Orthobunyaviruses are a group of viruses with significant public and veterinary health importance. These viruses are mainly transmitted through mosquito-, midge-, and tick-vectors, and are endemic to various regions of the world. Ebinur Lake virus (EBIV), a newly identified member of Orthobunyavirus, was isolated from Culex mosquitoes in Northwest China. In the present study, we aimed to characterize the pathogenesis and host immune responses of EBIV in BALB/c mice, as an animal model. Herein, we determined that BALB/c mice are highly susceptible to EBIV infection. The infected mice exhibited evident clinical signs including weight loss, mild encephalitis, and death. High mortality of mice was observed even with inoculation of one plaque-forming unit (PFU) of EBIV, and the infected mice succumbed to death within 5–9 days. After EBIV challenge, rapid viremic dissemination was detected in the peripheral tissues and the central nervous system, with prominent histopathologic changes observed in liver, spleen, thymus, and brain. Blood constituents’ analysis of EBIV infected mice exhibited leukopenia, thrombocytopenia, and significantly elevated ALT, LDH-L, and CK. Further, EBIV infection induced obvious cytokines changes in serum, spleen, and brain in mice. Collectively, our data describe the first study that systematically examines the pathogenesis of EBIV and induced immune response in an immunocompetent standard mouse model, expanding our knowledge of this virus, which may pose a threat to One Health.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…Virus titrations were performed using BHK-21 cells as previously described ( Xia et al, 2020 ), and the results expressed as PFU ml −1 .…”

Section: Methodsmentioning

confidence: 99%

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Pathogenesis and Immune Response of Ebinur Lake Virus: A Newly Identified Orthobunyavirus That Exhibited Strong Virulence in Mice

et al. 2021

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“…Ktedonobacteraceae is a novel taxonomic group that has only been recently added to the phylogenetic tree of Chloroflexi 30 , 31 . Their genome has been reported to be large and they are known to have a wide potential for different metabolic mechanisms 32 . Ktedonobacteraceae are mostly found in extreme environments, including volcanic ash and hydrothermal vents 30 .…”

Section: Discussionmentioning

confidence: 99%

Metagenomic analysis reveals rapid development of soil biota on fresh volcanic ash

Song

Kerfahi

Takahashi

et al. 2020

Sci Rep

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Little is known of the earliest stages of soil biota development of volcanic ash, and how rapidly it can proceed. We investigated the potential for soil biota development during the first 3 years, using outdoor mesocosms of sterile, freshly fallen volcanic ash from the Sakurajima volcano, Japan. Mesocosms were positioned in a range of climates across Japan and compared over 3 years, against the developed soils of surrounding natural ecosystems. DNA was extracted from mesocosms and community composition assessed using 16S rRNA gene sequences. Metagenome sequences were obtained using shotgun metagenome sequencing. While at 12 months there was insufficient DNA for sequencing, by 24 months and 36 months, the ash-soil metagenomes already showed a similar diversity of functional genes to the developed soils, with a similar range of functions. In a surprising contrast with our hypotheses, we found that the developing ash-soil community already showed a similar gene function diversity, phylum diversity and overall relative abundances of kingdoms of life when compared to developed forest soils. The ash mesocosms also did not show any increased relative abundance of genes associated with autotrophy (rbc, coxL), nor increased relative abundance of genes that are associated with acquisition of nutrients from abiotic sources (nifH). Although gene identities and taxonomic affinities in the developing ash-soils are to some extent distinct from the natural vegetation soils, it is surprising that so many of the key components of a soil community develop already by the 24-month stage. In this system, however, rapid development may be facilitated by the relatively moderate pH of the Sakurajima ash, proximity of our mesocosms to propagule sources, and the rapid establishment of a productive bryophyte and lichen layer on the surface. Ash from other volcanoes richer in acids or more distant from propagule sources could show a different pattern and slower soil biota development.

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A critical review on microbial degradation of petroleum-based plastics: quantitatively effects of chemical addition in cultivation media on biodegradation efficiency

Sun

Yusuf

et al. 2022

Biodegradation

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Characterization of Ebinur Lake Virus and Its Human Seroprevalence at the China–Kazakhstan Border

Cited by 20 publications

References 35 publications

Pathogenesis and Immune Response of Ebinur Lake Virus: A Newly Identified Orthobunyavirus That Exhibited Strong Virulence in Mice

Pathogenesis and Immune Response of Ebinur Lake Virus: A Newly Identified Orthobunyavirus That Exhibited Strong Virulence in Mice

Metagenomic analysis reveals rapid development of soil biota on fresh volcanic ash

A critical review on microbial degradation of petroleum-based plastics: quantitatively effects of chemical addition in cultivation media on biodegradation efficiency

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