Characterization of Drug Resistant Bacteria, Conjugal Transfer Efficiency and their Growth Kinetics Against Cassia Plants Leaf Extract

Abstract

In this present investigation, two Multi Antibiotics Resistant (MAR) bacterial strains namely SR2 and SR4 were isolated from clinical waste. The bacterial isolate SR2 was resistant to most of the antibiotics tested, but sensitive to levofloxacin. While, the other strain SR4 was sensitive to Cefixime and levofloxacin. Morphological, biochemical and 16S rRNA sequence analysis identified these 2 bacterial strains SR2 and SR4 as Acinetobacter sp. (GenBank Acc. No. KJ879241) and Aeromonas hydrophila (GenBank Acc. No. KJ879242), respectively. The conjugal transfer efficiency of antibiotics resistant gene to another bacterial strain was also tested, using Corynebacterium alkanolyticum ATH3 as a recipient. In order to control these pathogenic bacterial strains, leaf extracts obtained from three Cassia plants named C. siamea, C. allata and C. occidentalis were used at different concentration. Cassia siamea was recorded to be most effective against both of these bacterial strains. Growth kinetics of these two bacterial strains revealed that growth was decreased with gradual increase of concentration of leaf extract, obtained from C. siamea and ultimately negligible growth at 30 mg mLG 1 (3%).