Characterization of dog serum virome from Northeastern Brazil

Weber, Matheus Nunes; Cibulski, Samuel Paulo; Olegário, J.C.; Silva, M.S. da; Puhl, D.E.; Mósena, Ana Cristina Sbaraini; Alves, Christian Diniz Beduschi Travassos; Paim, Willian Pinto; Baumbach, Leticia Ferreira; Mayer, Fabiana Quoos; Fernandes, António Ramires; Azevêdo, Sérgio Santos de; Canal, Cláudio Wageck

doi:10.1016/j.virol.2018.09.023

Cited by 28 publications

(37 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…2). Recently, the circulation of CanineCV in Brazil was described 23 , although the sequences of this study could not be included in our analysis because they are partial sequences, the analysis make possible to assume that the Brazilian strains also share origin with Italian sequences supporting the Italian migration route of the CanineCV to South America 23 .…”

Section: Discussionmentioning

confidence: 94%

“…Later on, the virus was reported in Argentina in an outbreak of fatal gastroenteritis in www.nature.com/scientificreports/ dogs 25 , which was detected in multiple organs of the infected animals (pancreas, liver, kidney, lung, lymph nodes, spleen and large intestine). Since Kapoor et al, reported the detection of CanineCV for the first time in the serum of healthy dogs in the USA 1 , several studies have demonstrated the high circulation of CanineCV in different canine populations around world, with prevalences (or diagnostic rates) from 1 to 33% of the evaluated samples and it has been reported in almost all continents 20,21,23 . Our results strongly agree with the frequencies reported for northern China where the prevalence of CanineCV among diarrheic dogs was 15.6% 21 .…”

Section: Discussionmentioning

confidence: 99%

“…Based on this epidemiological issue, our results confirmed the wide viral circulation in all south American continent. The CanineCV was first described in Brazil in 1.34% of healthy dog serum samples in a canine virome study 23 . Later on, the virus was reported in Argentina in an outbreak of fatal gastroenteritis in dogs 25 , which was detected in multiple organs of the infected animals (pancreas, liver, kidney, lung, lymph nodes, spleen and large intestine).…”

Section: Discussionmentioning

confidence: 99%

“…It is well known that gastrointestinal disorders in canines are one of the most common diseases reported in clinics of companion animal and that these disease can be caused by a number of synergistic viral, bacterial and parasitic pathogens 20 . Recent molecular surveys have proposed the role of CanineCV not only as a causative agent but also it might play a role as a co-pathogen in the development of gastrointestinal disorders, mainly acting in synergism with other enteric viruses such as Canine Parvovirus-2 (CPV-2) 20 – 23 .…”

Section: Introductionmentioning

confidence: 99%

“…In Colombia, CPV-2 has been previously related as main cause of gastrointestinal disease in CPV-2 non-vaccine and partial vaccinated animals 24 . Considering that CanineCV has been recently reported in other Latin-American countries 23 , 25 and that it has never been reported in Colombia; the aim of this study was to confirm, for the first time, the presence of CanineCV in Colombia and to develop phylogenetic evolutive analyses of CanineCV in CPV-2 positive animals to understand the molecular epidemiology of this emerging virus in Colombia.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

First detection and full genomic analysis of Canine Circovirus in CPV-2 infected dogs in Colombia, South America

Giraldo-Ramirez

Rendón-Marín

Vargas-Bermúdez

et al. 2020

Sci Rep

View full text Add to dashboard Cite

Canine Circovirus (CanineCV) is an emerging virus which since its first report in USA in 2012, it has been described worldwide. It was the second mammalian circovirus species identified in dogs and its role in canine enteritis is still being uncertain as much as its association in disease with the Canine Parvovirus-2 (CPV-2). Here, we aim to confirm for the first time the presence of CanineCV in Colombia and to develop phylogenetic evolutive analyses of CanineCV in CPV-2 positive animals. DNA from samples were extracted and PCR, full genome sequencing and phylogenetic analysis was performed to detect and characterize CanineCV. From a total of 30 CPV-2 positive samples, 16.6% (n = 5) were positives for CanineCV. Sequencing analysis of Colombian CanineCV wild-type strains displayed high identity to each other (99.5–99.7% nt; 99.7% aa). The full genome phylogenetic analysis confirmed that worldwide reported CanineCV strains were separated into four distinct genotypes in addition to a European origin of the South American CanineCV strains. This study demonstrated the importance of continue surveillance of emerging viruses in canine populations and confirm for the first time the circulation and origin of CanineCV in Colombia.

show abstract

Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

First detection and full genomic analysis of Canine Circovirus in CPV-2 infected dogs in Colombia, South America

Giraldo-Ramirez

Rendón-Marín

Vargas-Bermúdez

et al. 2020

Sci Rep

View full text Add to dashboard Cite

show abstract

Family Genomoviridae: 2021 taxonomy update

Varsani

Krupovìč

2021

Arch Virol

View full text Add to dashboard Cite

The family Genomoviridae (phylum Cressdnaviricota, class Repensiviricetes, order Geplafuvirales) includes viruses with circular single-stranded DNA genomes encoding two proteins, the capsid protein and the rolling-circle replication initiation protein. The genomes of the vast majority of members in this family have been sequenced directly from diverse environmental or animal-and plant-associated samples, but two genomoviruses have been identified infecting fungi. Since the last taxonomic update of the Genomoviridae, a number of new members of this family have been sequenced. Here, we report on the most recent taxonomic update, including the creation of one new genus, Gemytripvirus, and classification of ~420 new genomoviruses into 164 new species. We also announce the adoption of the "Genus + freeform epithet" binomial system for the naming of all 236 officially recognized species in the family Genomoviridae. The updated taxonomy presented in this article has been accepted by the International Committee on Taxonomy of Viruses (ICTV).

show abstract

Establishment of hydrolysis probe system real-time PCR assay for rapid detection of canine circovirus

Sun

et al. 2021

3 Biotech

View full text Add to dashboard Cite

In the study, we established a hydrolysis probe-based real-time polymerase chain reaction (PCR) assay to rapidly detect Canine circovirus (CanineCV) DNA in faecal samples. We designed a pair of specific primers and one probe targeting Rep in CanineCV, and sensitivity, specificity, and repeatability tests were performed to evaluate the efficacy of the assay. The assay showed high sensitivity and a minimum detection limit of 8.42 × 10 1 copies/μL, which is 1000-fold more sensitive compared to traditional PCR. The method was also highly specific, without cross-reaction with other common canine viruses. Moreover, the assay showed high repeatability, and the mean intra-assay and inter-assay coefficients of variation were 0.26 and 0.36%, respectively. The results of the detection of clinical samples showed that the positive detection rate of CanineCV was 14.04% (8/57). Notably, 8% of clinical samples were co-infected with other canine pathogens. In conclusion, the establishment of a hydrolysis probe-based real-time PCR method provides a fast, sensitive, specific, reliable, and repeatable method for CanineCV detection. Supplementary Information The online version contains supplementary material available at 10.1007/s13205-021-03031-z.

show abstract

Characterization of dog serum virome from Northeastern Brazil

Cited by 28 publications

References 52 publications

First detection and full genomic analysis of Canine Circovirus in CPV-2 infected dogs in Colombia, South America

First detection and full genomic analysis of Canine Circovirus in CPV-2 infected dogs in Colombia, South America

Family Genomoviridae: 2021 taxonomy update

Establishment of hydrolysis probe system real-time PCR assay for rapid detection of canine circovirus

Contact Info

Product

Resources

About