Characterization of Dof Transcription Factors and the Heat-Tolerant Function of PeDof-11 in Passion Fruit (Passiflora edulis)

Chen, Ge; Xu, Yi; Jie, Gui; Huang, Ying; Ma, Funing; Wu, Wenhua; Han, Tongwen; Qiu, Wenwu; Liu, Yang; Song, Santai

doi:10.3390/ijms241512091

Cited by 4 publications

(1 citation statement)

References 30 publications

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“…Under salt stress, except for PgDOF8 , the expression of other PgDOFs genes in pearl millet leaves was up-regulated, and the up-regulation of PgDOF5 was the most significant ( Anup et al., 2017 ). Under high temperature stress in passion fruit, the majority of PeDOF members were inducible, and the expression of PeDOF11 was upregulated most significantly ( Chen et al., 2023 ). In walnut, research has revealed that JrDOF3 contributes to enhancing the heat stress response of JrGRAS2 .…”

Section: Discussionmentioning

confidence: 99%

Identification and functional analysis of the DOF gene family in Populus simonii: implications for development and stress response

Cai,

Xie,

Han

et al. 2024

Front. Plant Sci.

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BackgroundPopulus simonii, a notable native tree species in northern China, demonstrates impressive resistance to stress, broad adaptability, and exceptional hybridization potential. DOF family is a class of specific transcription factors that only exist in plants, widely participating in plant growth and development, and also playing an important role in abiotic stress response. To date, there have been no reported studies on the DOF gene family in P. simonii, and the expression levels of this gene family in different tissues of poplar, as well as its expression patterns under cold, heat, and other stress conditions, remain unclear.MethodsIn this study, DOF gene family were identified from the P. simonii genome, and various bioinformatics data on the DOF gene family, gene structure, gene distribution, promoters and regulatory networks were analyzed. Quantitative real time PCR technology was used to verify the expression patterns of the DOF gene family in different poplar tissues.ResultsThis research initially pinpointed 41 PSDOF genes in P. simonii genome. The chromosomal localization results revealed that the PSDOF genes is unevenly distributed among 19 chromosomes, with the highest number of genes located on chromosomes 4, 5, and 11. A phylogenetic tree was constructed based on the homology between Arabidopsis thaliana and P. simonii, dividing the 41 PSDOF genes into seven subgroups. The expression patterns of PSDOF genes indicated that specific genes are consistently upregulated in various tissues and under different stress conditions, suggesting their pivotal involvement in both plant development and response to stress. Notably, PSDOF35 and PSDOF28 serve as pivotal hubs in the interaction network, playing a unique role in coordinating with other genes within the family.ConclusionThe analysis enhances our comprehension of the functions of the DOF gene family in tissue development and stress responses within P. simonii. These findings provide a foundation for future exploration into the biological roles of DOF gene family.

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Section: Discussionmentioning

confidence: 99%

Identification and functional analysis of the DOF gene family in Populus simonii: implications for development and stress response

Cai,

Xie,

Han

et al. 2024

Front. Plant Sci.

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Expression patterns and evidence of the expansion of Nicotiana tabacum L. TALE genes in the Solanaceae family

Song,

Kong,

Dai

et al. 2024

Genet Resour Crop Evol

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The three-amino-acid-loop-extension (TALE) proteins are a class of transcription factors that regulate meristem differentiation during plant growth and development. In this study, we identified a total of 45 members of the tobacco TALE (NtTALE) transcription factor family. Based on domain type, the NtTALE genes were divided into BELL subfamily and KNOX subfamily. Cis-acting element analysis showed that the NtTALE gene promoters had a variety of response elements, including light-responsive elements, hormonal and abiotic stresses responsive elements, plant development related elements. Among the 45 NtTALE genes, the expression patterns of various plant tissues at different stages were different, 10 responded to alkali stress, 19 responded to dehydration treatment, 14 responded to cold stress, two responded to salt stress, and 17 responded to Ralstonia solanacearum infection. Phylogenetic analysis showed that the number of TALE genes in Nicotiana was significantly expanded compared with that in other Solanaceae. The KNOXIII subfamily members (NtTALE29, NtTALE45) were found only in Nicotiana tabacum. This study provides a theoretical basis for further research on the biological function of NtTALE genes.

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Genome-Based Identification of the Dof Gene Family in Three Cymbidium Species and Their Responses to Heat Stress in Cymbidium goeringii

He,

Zhang,

Huang

et al. 2024

IJMS

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As an important genus in Orchidaceae, Cymbidium has rich ecological diversity and significant economic value. DNA binding with one zinc finger (Dof) proteins are pivotal plant-specific transcription factors that play crucial roles in the growth, development, and stress response of plants. Although the Dof genes have been identified and functionally analyzed in numerous plants, exploration in Orchidaceae remains limited. We conducted a thorough analysis of the Dof gene family in Cymbidium goeringii, C. ensifolium, and C. sinensis. In total, 91 Dof genes (27 CgDofs, 34 CeDofs, 30 CsDofs) were identified, and Dof genes were divided into five groups (I–V) based on phylogenetic analysis. All Dof proteins have motif 1 and motif 2 conserved domains and over half of the genes contained introns. Chromosomal localization and collinearity analysis of Dof genes revealed their evolutionary relationships and potential gene duplication events. Analysis of cis-elements in CgDofs, CeDofs, and CsDofs promoters showed that light-responsive cis-elements were the most common, followed by hormone-responsive elements, plant growth-related elements, and abiotic stress response elements. Dof proteins in three Cymbidium species primarily exhibit a random coil structure, while homology modeling exhibited significant similarity. In addition, RT-qPCR analysis showed that the expression levels of nine CgDofs changed greatly under heat stress. CgDof03, CgDof22, CgDof27, CgDof08, and CgDof23 showed varying degrees of upregulation. Most upregulated genes under heat stress belong to group I, indicating that the Dof genes in group I have great potential for high-temperature resistance. In conclusion, our study systematically demonstrated the molecular characteristics of Dof genes in different Cymbidium species, preliminarily revealed the patterns of heat stress, and provided a reference for further exploration of stress breeding in orchids.

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Characterization of Dof Transcription Factors and the Heat-Tolerant Function of PeDof-11 in Passion Fruit (Passiflora edulis)

Cited by 4 publications

References 30 publications

Identification and functional analysis of the DOF gene family in Populus simonii: implications for development and stress response

Identification and functional analysis of the DOF gene family in Populus simonii: implications for development and stress response

Expression patterns and evidence of the expansion of Nicotiana tabacum L. TALE genes in the Solanaceae family

Genome-Based Identification of the Dof Gene Family in Three Cymbidium Species and Their Responses to Heat Stress in Cymbidium goeringii

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