Characterization of DNA from three bee species

Jordan, Ronald A.; Brosemer, Ronald W.

doi:10.1016/0022-1910(74)90035-3

Cited by 36 publications

(19 citation statements)

References 15 publications

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“…1c-d (Jordan and Brosemer, 1974), 262 Mbp (0.268 pg) (The Honeybee Genome Sequencing Consortium, 2006) and 625 Mbp (0.639 pg) (Wilfert et al, 2006). The genome size of these two Melipona species is also larger than the mean Cytogenetic studies have allowed the division of several Melipona species with the same number of chromosomes (2n = 18) in two groups based on their heterochromatin content.…”

Section: Discussionmentioning

confidence: 99%

“…Most of the 602 insect C-values measured to date, however, belong to the orders Diptera, Coleoptera, Orthoptera and Hemiptera (Gregory, 2008). Specifically in Hymenoptera, one of the largest insect orders, with approximately 115.000 species described (La Salle and Gauld, 1993), the genome size has been measured in fifty two species: four bees (Jordan and Brosemer, 1974;Crain et al, 1976;Rasch, 1985;Petitpierre, 1996;Gadau et al, 2001;Wilfert et al, 2006;The Honeybee Genome Sequencing Consortium, 2006), six wasps (Rasch et al, 1975(Rasch et al, , 1977Rasch, 1985;Johnston et al, 2004;Barcenas et al, 2008) and forty two ants (Li and Heinz, 2000;Johnston et al, 2004;Tsutsui et al, 2008). The knowledge of the genome size provides data for comparative studies in a variety of taxonomic levels and groups, for phylogenetic associations and for the design of sequencing projects.…”

Section: Introductionmentioning

confidence: 99%

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Genome size estimation of three stingless bee species (Hymenoptera, Meliponinae) by flow cytometry

et al. 2009

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-The present study standardizes a reproducible flow cytometry (FCM) protocol for DNA content measurement of bee species and applied it to Scaptotrigona and Melipona species. The mean nuclear DNA content value of male and female S. xantotricha was 0.42 pg (410.8 Mbp) and 0.44 pg (430.3 Mbp), respectively, while the mean haploid genome size was determined to be 0.93 pg (909.5 Mbp) for M. rufiventris and 0.95 pg (929.1 Mbp) for M. mondury. The variation observed in this study, albeit in a preliminary way, may be related with the variation in the heterochromatin content in the chromosomes of Scaptotrigona and Melipona species. The results provide a starting point for comparative analysis on the patterns of genome size variation in the stingless bees.flow cytometry / genome size / Hymenoptera / stingless bees

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Genome size estimation of three stingless bee species (Hymenoptera, Meliponinae) by flow cytometry

et al. 2009

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“…The large discrepancies between previously reported and current estimates for A. mellifera and B. terrestris are because of these methodological issues. The original estimate for the honeybee was derived through CsCl-gradient centrifugation (Jordan and Brosemer, 1974), which is generally no longer considered adequate (Dolezel et al, 1998). Although the bumblebee estimate was derived using flow cytometry, the inaccurate honeybee estimate was used as a standard, and DAPI was used as a stain.…”

Section: Methodsmentioning

confidence: 99%

“…Furthermore, much of the data from previous studies had to be revised considerably. For example, the estimate of the physical genome size of the honeybee increased from an initial 178 Mb (Jordan and Brosemer, 1974) to 262 Mb (The Honeybee Genome Consortium, 2006), and from 274 Mb (Gadau et al, 2000) to 625 Mb in the bumblebee, Bombus terrestris (Wilfert et al, 2006) owing to more accurate analyses and the complete sequencing of the honeybee genome. Therefore, a re-evaluation of the genomic data, including recombination rates of honeybees and other social Hymenoptera as compared to other taxa, has become necessary.…”

Section: Introductionmentioning

confidence: 99%

Variation in genomic recombination rates among animal taxa and the case of social insects

2007

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Meiotic recombination is almost universal among sexually reproducing organisms. Because the process leads to the destruction of successful parental allele combinations and the creation of novel, untested genotypes for offspring, the evolutionary forces responsible for the origin and maintenance of this counter-intuitive process are still enigmatic. Here, we have used newly available genetic data to compare genome-wide recombination rates in a report on recombination rates among different taxa. In particular, we find that among the higher eukaryotes exceptionally high rates are found in social Hymenoptera. The high rates are compatible with current hypotheses suggesting that sociality in insects strongly selects for increased genotypic diversity in worker offspring to either meet the demands of a sophisticated caste system or to mitigate against the effects of parasitism. Our findings might stimulate more detailed research for the comparative study of recombination frequencies in taxa with different life histories or ecological settings and so help to understand the causes for the evolution and maintenance of this puzzling process.

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“…Subsequently, the physical size of the honeybee genome has been revised from the originally published 178 Mb (Jordan and Brosemer 1974) to 265 Mb (J.S. Johnston, Texas A&M University, Texas, personal communication).…”

Section: Genome Sizementioning

confidence: 99%

A core linkage map of the bumblebeeBombus terrestris

Wilfert

Gadau

Schmid‐Hempel

2006

Genome

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The bumblebee Bombus terrestris is an economically important pollinator and an emerging model species in quantitative and population genetics. We generated genetic linkage maps for 3 independent mapping populations of B. terrestris. The linkage map with the highest resolution had 21 linkage groups, which adequately represents the haploid chromosome number of B. terrestris (n = 18). This map can be considered saturated, with an average marker distance of 10.3 cM and an estimated genome coverage of 81%. Using flow cytometry, we have estimated the genome size of this species to be 625 Mb. With an estimated total recombination genome length of 2760 cM, this results in a ratio of 226 kb/cM between the physical and genetic genome sizes. A recurring set of microsatellites and amplified fragment length polymorphism (AFLP) markers allowed the alignment of 14 linkage groups between the 3 maps. We propose to adopt this core map as a reference tool for future genetic and molecular work in B. terrestris.

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Characterization of DNA from three bee species

Cited by 36 publications

References 15 publications

Genome size estimation of three stingless bee species (Hymenoptera, Meliponinae) by flow cytometry

Genome size estimation of three stingless bee species (Hymenoptera, Meliponinae) by flow cytometry

Variation in genomic recombination rates among animal taxa and the case of social insects

A core linkage map of the bumblebeeBombus terrestris

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