Characterization of digenetic trematodes infecting African catfish (Clarias gariepinus) based on integrated morphological, molecular, histopathological, and immunological examination

Attia, Marwa M.; Abdelsalam, Mohamed; Korany, Reda M. S.; Mahdy, Olfat A.

doi:10.1007/s00436-021-07257-x

Cited by 20 publications

(10 citation statements)

References 50 publications

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“…The tissue specimens from the positive samples were fixed in 10% formalin, dehydrated in ascending alcohol concentrations (50%, 70%, 90%, 95%, absolute ethanol), cleared with xylene and embedded in paraffin blocks. Then, the specimens were stained by hematoxylin and eosin and examined microscopically [ 41 ].…”

Section: Methodsmentioning

confidence: 99%

Prevalence and Morphological Investigation of Parasitic Infection in Freshwater Fish (Nile Tilapia) from Upper Egypt

Abd-ELrahman

Gareh

Mohamed

et al. 2023

Animals

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Fish are a source of high-quality protein with low cholesterol, but they are susceptible to parasitic infections, which have a significant impact on aquaculture, in addition to their zoonotic potential. The present study estimated parasitic infections and evaluated the diversity of zoonotic parasites in freshwater Nile tilapia (Oreochromis niloticus) in Assiut Governorate, Upper Egypt. A total of 300 samples were randomly collected from the Assiut Governorate. These fish were examined for both ectoparasites and endoparasites, followed by the experimental infection of mice with encysted metacercariae (EMC) for the retrieval of the adult worms. The overall prevalence of the variable parasites was 82% (246 of 300). Both ecto- and endoparasites were detected in 41% (123 of 300) of the examined fish. The identified ectoparasites were Gyrodactylus, Dactylogrus, Cichlidogyrus, Trichodina and Icthyophthirius multifiliis, in 5%, 4%, 22%, 6% and 4% of the fish, respectively. The endoparasites were trematodes (Orientocreadium batrachoides 3%), nematodes (Contracaecum. 2%), acanthocephala (Acanthosentis tilapiae 25%) and protozoa that included Isospora and Eimeria spp., in 1% and 8% of fish, respectively. Myxobolus was detected in 2% of the examined fish. The overall prevalence of encysted metacercariae (EMC) was 95% (285 of 300), while infection with macroscopic EMC had a prevalence of 37% and microscopic EMC had a prevalence of 58%. The adult worms recovered from the experimental infections were Prohemistomum vivax and Mesostephanus spp., which belong to the family Cyathocotylidae. Collectively, these findings reflect the relatively high occurrence of parasites among the studied fish, confirming the necessity of strict measures to control infection.

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Section: Methodsmentioning

confidence: 99%

Prevalence and Morphological Investigation of Parasitic Infection in Freshwater Fish (Nile Tilapia) from Upper Egypt

Abd-ELrahman

Gareh

Mohamed

et al. 2023

Animals

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“…A Step One™ Real-time PCR System (Applied Biosystems, USA) was used to perform quantitative PCR tests. In the reaction, 10 µL SYBR® Premix Ex Taq™ (Tli RNase H Plus), ROX plus (TaKaRa, Japan), 1 µL cDNA, and 0.5 μL of forward and reverse primers (100 nM) were combined and filled to a final volume of 20 μL with ultrapure water [ 35 ]. The RT-PCR methodology was run as follows: denaturation for 30 s at 94°C, annealing for 30 s at 60°C, and extension for 45 s at 72°C, in a 40-cycle amplification.…”

Section: Methodsmentioning

confidence: 99%

Mass kills in hatchery-reared European seabass (Dicentrarchus labrax) triggered by concomitant infections of Amyloodinium ocellatum and Vibrio alginolyticus

Ragab

Elgendy

Sabry

et al. 2022

International Journal of Veterinary Science and Medicine

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Amyloodiniosis and vibriosis are serious diseases in European seabass ( Dicentrarchus labrax ) hatcheries with noticeable high mortality. This study was conducted on tank-cultured D. labrax frys at a private marine hatchery near Mariout Lake (Alexandria, Egypt). Frys showed a high mortality rate (70%), lethargy, darkening, asphyxia, ascites, and velvety skin appearance. Both infectious agents were presumptively identified in all investigated frys. The identities of the two recovered agents were confirmed by molecular assay and phylogenetic analysis. On the tissue level, histopathological examination of skin, splenic, and renal tissue indicated severe alterations due to the direct impacts of both infections. On the cellular level, scanning electron micrographs showed both protozoal and bacterial pathogens on/in gill epithelial cells in solitary and colonial forms. Vibrio alginolyticus showed variable results for tested antibiotics, with a higher sensitivity to florfenicol. A successful control strategy was strictly adopted to overcome infections and stop mortalities. Copper sulphate and hydrogen peroxide were efficiently applied to tank water to overcome A. ocellatum infections. Further, florfenicol was effectively used to overcome systemic V. alginolyticus infections. The efficacy of treatments was confirmed by the absence of infectious agents in randomly collected fish samples. To the best of the authors’ knowledge, this study is one of the earliest Egyptian studies that dealt with the dilemma of mass kills associated with external parasitic/systemic bacterial infections among hatchery-reared European seabass.

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“…The approach described by Attia et al [45][46][47] was followed for the extraction and synthesis of the analyzed mRNA. In a Step One™ Real-Time PCR System (Applied Biosystems, Bedford, MA, USA).…”

Section: Genesmentioning

confidence: 99%

“…The used cycling conditions were followed by Attia et al [45]. The ∆ CT value was determined by subtracting the controlled gene (β-actin), ∆ CT, as an internal control, from the result of the examined gene.…”

Section: Genesmentioning

confidence: 99%

The Biological Activity of Illicium verum (Star Anise) on Lernaea cyprinacea-Infested Carassius auratus (Goldfish): In Vivo Study

Attia

Alzahrani

Hanna

et al. 2022

Life

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Lernaea cyprinacea infestation is considered a serious economic problem in the fish market. An assessment to control this parasite is needed to manage this problem. The Illicium verum oil extract has considerable antioxidant activity and scavenges 96.22% of free radicals; the high antioxidant activity refers to the phenolic content presence. The extract contains minerals, especially K, fibers, and dry matter. So, the Illicium verum ingredients were tested against this copepod for in vitro and in vivo investigation with the assessment of the treatment trial using a scanning electron microscope and evaluating the change in different immunological genes in goldfish. Female parasitic L. cyprinacea worms were blackish and hairy. The in vitro study on L. cyprinacea adults using star anise revealed that the LC50 was 12.5 and 25 μg/mL for 2 and 1 h exposure periods, respectively. Interleukin (IL-1β) and IL-6 were grossly upregulated in C. auratus-infested skin by L. cyprinacea after treatment by 1 week, then declined after 3 weeks. In contrast, TNF-α was 18 folds upregulated in the first week after treatment, with a decline after 3 weeks. In conclusion, star anise is recommended as a safe and economical agent for controlling L. cyprinacea infestation in fish.

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Characterization of digenetic trematodes infecting African catfish (Clarias gariepinus) based on integrated morphological, molecular, histopathological, and immunological examination

Cited by 20 publications

References 50 publications

Prevalence and Morphological Investigation of Parasitic Infection in Freshwater Fish (Nile Tilapia) from Upper Egypt

Prevalence and Morphological Investigation of Parasitic Infection in Freshwater Fish (Nile Tilapia) from Upper Egypt

Mass kills in hatchery-reared European seabass (Dicentrarchus labrax) triggered by concomitant infections of Amyloodinium ocellatum and Vibrio alginolyticus

The Biological Activity of Illicium verum (Star Anise) on Lernaea cyprinacea-Infested Carassius auratus (Goldfish): In Vivo Study

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