Characterization of different tissue changes in normal, betel chewers, potentially malignant lesions, conditions and oral squamous cell carcinoma using reflectance confocal microscopy: Correlation with routine histopathology

Anuthama, Krishnamurthy; Sherlin, Herald J; Anuja, N; Ramani, Pratibha; Premkumar, Priya; Chandrasekar, Thiruvengadam

doi:10.1016/j.oraloncology.2009.12.008

Cited by 17 publications

(17 citation statements)

References 10 publications

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“…[8][9][10][11][12][13][14][15][16] Compared with the wider use of the in vivo confocal microscopy, ex vivo tissue assessment has been performed mainly in skin oncology to examine excision margins in Mohs surgery; 17 only few studies have focused on different tissues. [18][19][20][21][22][23][24][25][26] In surgical oncology, the possibility to obtain a reliable histopathologic diagnosis in a short time represents a relevant clinical advantage for patient management. Nowadays histopathologic diagnosis is routinely rendered through intraoperative frozen tissue examination when a rapid diagnosis is required.…”

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confidence: 99%

Fluorescence confocal microscopy for pathologists

et al. 2014

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Confocal microscopy is a non-invasive method of optical imaging that may provide microscopic images of untreated tissue that correspond almost perfectly to hematoxylin- and eosin-stained slides. Nowadays, following two confocal imaging systems are available: (1) reflectance confocal microscopy, based on the natural differences in refractive indices of subcellular structures within the tissues; (2) fluorescence confocal microscopy, based on the use of fluorochromes, such as acridine orange, to increase the contrast epithelium-stroma. In clinical practice to date, confocal microscopy has been used with the goal of obviating the need for excision biopsies, thereby reducing the need for pathological examination. The aim of our study was to test fluorescence confocal microscopy on different types of surgical specimens, specifically breast, lymph node, thyroid, and colon. The confocal images were correlated to the corresponding histological sections in order to provide a morphologic parallel and to highlight current limitations and possible applications of this technology for surgical pathology practice. As a result, neoplastic tissues were easily distinguishable from normal structures and reactive processes such as fibrosis; the use of fluorescence enhanced contrast and image quality in confocal microscopy without compromising final histologic evaluation. Finally, the fluorescence confocal microscopy images of the adipose tissue were as accurate as those of conventional histology and were devoid of the frozen-section-related artefacts that can compromise intraoperative evaluation. Despite some limitations mainly related to black/white images, which require training in imaging interpretation, this study confirms that fluorescence confocal microscopy may represent an alternative to frozen sections in the assessment of margin status in selected settings or when the conservation of the specimen is crucial. This is the first study to employ fluorescent confocal microscopy on surgical specimens other than the skin and to evaluate the diagnostic capability of this technology from pathologists' viewpoint.

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confidence: 99%

Fluorescence confocal microscopy for pathologists

et al. 2014

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“…Other features of noncancerous and cancerous oral tissue that could be identified in the confocal images included areas of inflammation, fibrosis, muscle fibers, and salivary glands [62] . Additional studies have evaluated the use of CFM for detection of oral malignancy although to the best of the authors knowledge, no clinical trials evaluating the technology for intraoperative margin control have been reported to date [22,63] . In a recent review, Maher et al noted that current device developments for oral CFM include improved probe design and did suggest that improvements could allow CFM to play a role in the intra-operative mapping for cancer surgery [64] .…”

Section: Confocal Microscopy (Cfm)mentioning

confidence: 99%

How Close Are We to Real Time Optical Margin Control in Head and Neck Oncologic Surgery?

Miles

2016

Cancer Cell Microenviron

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The quest for negative margins during head and neck oncologic surgery continues to challenge surgeons worldwide. Current technological advances offer significant promise to advance the field and improve operative margin detection. This review focuses on current optical technology, which has been evaluated for intraoperative margin control in head and neck oncologic surgery. Many of the current systems have technical limitations and there is no current standard technology being applied in the field of head and neck oncology to enhance intraoperative margin control. Surgeons continue to rely on intraoperative frozen section analysis, however many optical systems have shown high rates of sensitivity and specificity when discriminating benign from malignant tissue. These technologies shows significant promise for intraoperative margin control and may be enhanced by targeted molecular imaging, or spectral analysis, in the future. Translational trials are rare and are the key to the path of independence from frozen section analysis.

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“…Quadro 2.1 -Aspectos observados a MCR da mucosa labial normal (White et al,1999) ............................................................................................... Quadro 2.2 -Aspectos observados a MCR da mucosa lingual normal (White et al,1999) (Anuthama et al,2010) ....…”

Section: Lista De Quadrosunclassified

“…Quadro 2.7 -Aspectos do carcinoma epidermoide por meio da MCR (Anuthama et al,2010) Quadro 2.8 -Aspectos do penfigoide das membranas mucosas, pênfigo vulgar e líquen plano oral por meio da MCR (adaptado de Alessi et al, 2013)57 Quadro 4.1 -Critérios considerados no exame de MCR (Rishpon et al, 2009) (Brasil, 2014).…”

Section: Lista De Quadrosunclassified

“…A solução de lugol também é indicada como auxiliar durante o trans operatório para identificar a extensão da lesão e suas margens permitindo uma redução na recorrência da lesão no mesmo local (Kanatas et al, 2011;Kurita et al, 2012) e aumento da sobrevida dos pacientes com carcinoma epidermoide de língua (Umeda et al, 2011 , para avaliação das margens da lesão, para seleção do melhor local para biópsia e protocolos de exames têm sido ativamente pesquisados (Ulrich et al, 2007(Ulrich et al, , 2008Gonzalez, 2009;Anuthama et al, 2010;Rao et al, 2013;Ardigo et al, 2014).…”

Section: Corante Azul De Toluidinaunclassified

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Análise histopatológica de lesões leucoqueratósicas da mucosa oral avaliadas pelos exames de luminescência, cromoscopia e microscopia confocal reflectante

Bezerra¹

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Characterization of different tissue changes in normal, betel chewers, potentially malignant lesions, conditions and oral squamous cell carcinoma using reflectance confocal microscopy: Correlation with routine histopathology

Cited by 17 publications

References 10 publications

Fluorescence confocal microscopy for pathologists

Fluorescence confocal microscopy for pathologists

How Close Are We to Real Time Optical Margin Control in Head and Neck Oncologic Surgery?

Análise histopatológica de lesões leucoqueratósicas da mucosa oral avaliadas pelos exames de luminescência, cromoscopia e microscopia confocal reflectante

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