Characterization of Dickeya and Pectobacterium strains obtained from diseased potato plants in different climatic conditions of Norway and Poland

Dees, Merete Wiken; Lebecka, Renata; Perminow, Juliana; Czajkowski, Robert; Grupa, Anna; Motyka, Agata; Żołędowska, Sabina; Śliwka, Jadwiga; Łojkowska, Ewa; Brurberg, May Bente

doi:10.1007/s10658-016-1140-2

Cited by 46 publications

(46 citation statements)

References 38 publications

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“…Different pathogen species are often isolated from diseased tubers within the same affected crops (33) and the same potato tubers (34). Therefore, it is plausible that multispecies lesions can occur in nature.…”

Section: Discussionmentioning

confidence: 99%

Signal Integration in Quorum Sensing Enables Cross-Species Induction of Virulence in Pectobacterium wasabiae

Valente

Nadal‐Jimenez

Carvalho

et al. 2017

mBio

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Bacterial communities can sense their neighbors, regulating group behaviors in response to cell density and environmental changes. The diversity of signaling networks in a single species has been postulated to allow custom responses to different stimuli; however, little is known about how multiple signals are integrated and the implications of this integration in different ecological contexts. In the plant pathogen Pectobacterium wasabiae (formerly Erwinia carotovora), two signaling networks—the N-acyl homoserine lactone (AHL) quorum-sensing system and the Gac/Rsm signal transduction pathway—control the expression of secreted plant cell wall-degrading enzymes, its major virulence determinants. We show that the AHL system controls the Gac/Rsm system by affecting the expression of the regulatory RNA RsmB. This regulation is mediated by ExpR2, the quorum-sensing receptor that responds to the P. wasabiae cognate AHL but also to AHLs produced by other bacterial species. As a consequence, this level of regulation allows P. wasabiae to bypass the Gac-dependent regulation of RsmB in the presence of exogenous AHLs or AHL-producing bacteria. We provide in vivo evidence that this pivotal role of RsmB in signal transduction is important for the ability of P. wasabiae to induce virulence in response to other AHL-producing bacteria in multispecies plant lesions. Our results suggest that the signaling architecture in P. wasabiae was coopted to prime the bacteria to eavesdrop on other bacteria and quickly join the efforts of other species, which are already exploiting host resources.

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Section: Discussionmentioning

confidence: 99%

Signal Integration in Quorum Sensing Enables Cross-Species Induction of Virulence in Pectobacterium wasabiae

Valente

Nadal‐Jimenez

Carvalho

et al. 2017

mBio

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“…In this study, we selected the most aggressive bacterial isolates to potato tubers of the susceptible cultivar Irys at a temperature of 26°C from our previous studies (Wiken Dees et al, 2017) Latour et al (2007), the optimum temperature for Pba pathogenicity is 20°C, as a result of the optimal temperature for efficient production of lytic enzymes in Pba of between 12°C and 24°C and the optimal temperatures for growth of between 26°C and 27°C. The lower production of PCWDEs at higher temperatures may be partly compensated by increasing population size because of higher MF.…”

Section: Temperature Effects On the Ds Of Potato Tubersmentioning

confidence: 99%

“…The goal of this study was to determine the optimal temperature range between 18°C and 30°C (representing the soil temperature during the vegetation period) for disease development in potato tubers and to identify the association between disease development and the in vitro growth rate of three species of bacteria, Pba, Pcc and Dsol. These species differ in the range of their optimal growth temperatures and are highly aggressive to potato tubers (Wiken Dees et al, 2017). Each of the species was represented by three isolates.…”

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confidence: 99%

“…The isolates representing the three species used in this experiment (except the strain Pcc3) were recently isolated in Poland and Norway. The isolates were used in our previous studies and are characterized by their high level of aggressiveness to potato tubers in the susceptible cultivar Irys at 26°C(Wiken Dees et al, 2017). The multiplication of bacteria in vitro increases significantly with temperature in the range 18°C-30°C for two species, Pcc and Dsol, and for Pba, the growth was slower in the range 26°C-30°C.…”

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confidence: 99%

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Comparison of temperature effects on the in vitro growth and disease development in potato tubers inoculated with bacteria Pectobacterium atrosepticum, P carotovorum subsp. carotovorum and Dickeya solani

Lebecka

Flis

Murawska

2018

Journal of Phytopathology

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The relationship between the rate of in vitro growth of bacterial isolates of Pectobacterium atrosepticum, P. carotovorum subsp. carotovorum and Dickeya solani and their pathogenicity was investigated in tubers of two potato cultivars at four temperatures ranging from 18°C to 30°C. The rate of in vitro growth was highly positively correlated with the number of rotted tubers (r ranged from 0.91 to 0.93) and with the weight of macerated potato tissue, which, however, was only found for P. carotovorum and D. solani (r = 0.76; r = 0.91, respectively) and not for P. atrosepticum. The weight of macerated tissue increased with the temperature, but significant differences between species of bacteria were observed only at 26°C and above, at which temperatures D. solani was the most aggressive, followed by P. carotovorum and P. atrosepticum. Almost all potato tubers inoculated with bacteria showed symptoms of soft rot at 26°C and 30°C, but the number of rotting tubers at lower temperatures (22°C and 18°C) decreased significantly. The lowest disease incidence, 11% of tubers with symptoms, was observed for the D. solani and cultivar Sonda at 18°C, what was also confirmed in a separate experiment with tubers from four potato cultivars inoculated with the highly aggressive isolate of D. solani. At temperatures from 18°C to 30°C, the differences in disease severity between potato cultivars with various resistance to bacteria increased in line with temperature, while the differences in disease incidence decreased.

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“…Blackleg and soft rot of potato cause heavy losses of potato production and always occur in the field as well as during storage and transportation. Soft rot of tubers has been shown to be initiated at the stolon end, wounds or through lenticels (Dees et al, ). The symptoms of soft rot and blackleg disease are curled upper leaves, compact foliage, stunting and leaves fading from green to yellow‐green followed by blackleg symptoms on lower stems.…”

Section: Introductionmentioning

confidence: 99%

Characterization of blackleg and soft rot from potato in northern Thailand

Kumvinit

Akarapisan

2019

Journal of Phytopathology

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Blackleg and soft rot of potato cause economic loss through reduced yield and quality. The causal agents of bacterial blackleg and soft rot of potato were identified based on biological data and sequence analyses of the 16S rDNA gene. Between 2016 and 2018, diseased potato stems and tubers were collected in Chai Prakan District, Chiang Mai Province, and Chiang Khum District, Pa Yao Province. The symptoms included black stem lesions, soft rot on tubers, wilting, break down of the stem vascular ring and foliar yellowing. Of 13 bacterial isolates, five were identified as Pectobacterium carotovorum subsp. brasiliense, four‐Dickeya dadantii, two‐Pseudomonas putida and two‐Bacillus altitudinis. Pathogenicity tests of P. carotovorum subsp. brasiliense and D. dadantii resulted in lower leaves turning yellow and wilting followed by blackleg symptoms on lower stems and maceration of tuber tissue. Symptoms caused by P. putida were yellowing and wilting of leaves. B. altitudinis caused yellowing of the lower leaves and wilting followed by drying of leaf tissue. This is a first report of these bacterial pathogens causing blackleg and soft rot of potato in Thailand.

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Characterization of Dickeya and Pectobacterium strains obtained from diseased potato plants in different climatic conditions of Norway and Poland

Cited by 46 publications

References 38 publications

Signal Integration in Quorum Sensing Enables Cross-Species Induction of Virulence in Pectobacterium wasabiae

Signal Integration in Quorum Sensing Enables Cross-Species Induction of Virulence in Pectobacterium wasabiae

Comparison of temperature effects on the in vitro growth and disease development in potato tubers inoculated with bacteria Pectobacterium atrosepticum, P carotovorum subsp. carotovorum and Dickeya solani

Characterization of blackleg and soft rot from potato in northern Thailand

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