Characterization of di‐ and tetranucleotide microsatellite markers in rainbow smelt (<i>Osmerus mordax</i>)

Coulson, Mark W.; Paterson, Ian G.; Green, A.; Kepkay, Rosemarie; Bentzen, Paul

doi:10.1111/j.1471-8286.2006.01409.x

Cited by 14 publications

(9 citation statements)

References 12 publications

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“…A final extension was held at 72° C for 5 min. Reactions were run on Eppendorf thermocyclers and imaged on an FMBioII system (Hitachi Genetic Systems, Alameda, CA, U.S.A.) [see Coulson et al (2006) for further details].…”

Section: Methodsmentioning

confidence: 99%

Morphological and genetic differentiation in anadromous smelt Osmerus mordax (Mitchill): disentangling the effects of geography and morphology on gene flow

Bradbury

Coulson

Campana

et al. 2006

Journal of Fish Biology

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Morphological analyses were combined with genetic analyses at nine microsatellite loci to examine the determinants of gene flow at 21 spawning locations of rainbow smelt Osmerus mordax along the east coast of Canada. Associations between morphology, geography and gene flow were examined using a computational geometric approach and partial Mantel tests. Significant barriers to gene flow and discontinuities in morphology were observed between Newfoundland and mainland Canada, as well as within Newfoundland samples. On regional scales, contrasting patterns were present with restricted gene flow between Newfoundland populations (F ST ¼ c. 0Á11) and high gene flow between mainland populations (F ST ¼ c. 0Á017). Within Newfoundland populations, geographic distance was significantly associated with gene flow (r ¼ 0Á85, P < 0Á001) contrasting mainland samples where gene flow was most associated with phenotypic divergence (r ¼ 0Á33, P < 0Á001). At large spatial scales, weak (r ¼ 0Á19, P ¼ 0Á02) associations between gene flow and geographic distance were observed, and moderate associations were also observed between gene flow and morphology (r ¼ 0Á28, P < 0Á001). The presence of significant genetic isolation by distance in Newfoundland samples and the clear discontinuity associated with the Cabot Strait suggest geography may be the primary determinant of gene flow. Interestingly, the association between genetic and morphological divergence within mainland samples and overall, supports the hypothesis that gene flow may be moderated by morphological divergence at larger spatial scales even in high gene flow environments.

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Section: Methodsmentioning

confidence: 99%

Morphological and genetic differentiation in anadromous smelt Osmerus mordax (Mitchill): disentangling the effects of geography and morphology on gene flow

Bradbury

Coulson

Campana

et al. 2006

Journal of Fish Biology

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“…For microsatellites, the following 13 loci were used: Omo1, Omo2, Omo3, Omo4, Omo5, Omo6a, Omo9, Omo11, Omo13, Omo14, Omo15, and Omo16 (Coulson et al 2006). Primers for Omo6a amplified a second locus, Omo6b, which was also scored and examined.…”

Section: Laboratory Methodsmentioning

confidence: 99%

“…Reactions were run on Eppendorf thermocyclers and imaged on an FMBioII system (Hitachi Genetic Systems). See Coulson et al (2006) for further details regarding PCR conditions. This work builds on a previous microsatellite study which examined variation in 8 of these loci (Omo1, Omo2, Omo3, Omo4, Omo5, Omo9, Omo15, and Omo16).…”

Section: Laboratory Methodsmentioning

confidence: 99%

Contemporary nuclear and mitochondrial genetic clines in a north temperate estuarine fish reflect Pleistocene vicariance

Bradbury¹,

Coulson²,

Campana³

et al. 2011

Mar. Ecol. Prog. Ser.

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Contemporary genetic spatial structure in north temperate marine species is likely the culmination of multiple vicariant and dispersive cycles. Here we evaluate spatial genetic structure in an estuarine fish, rainbow smelt Osmerus mordax, from coastal Newfoundland, Canada, using data from both mtDNA (ND5) sequences and nuclear loci (11 microsatellites). Sequence analysis of ND5 identified a previously unrecognized genetic discontinuity between 2 hypothesized glacial clades in southeastern Newfoundland. Microsatellite based tests for directional selection identified a locus (Omo11, p < 0.001) that mirrored mtDNA clades in the geographic distribution of its 2 common alleles but did not display elevated differentiation following correction for heterozygosity. Bayesian multilocus clustering of the remaining micro satellite loci supported the presence of 2 predominant groups, for which the spatial distribution was also largely consistent with those of the mtDNA and Omo11 clades. Taken together, the similarity in microsatellite and mtDNA clines supports the hypothesis that contemporary spatial structure in smelt reflects historical landscape isolation maintained by low dispersal and selective processes producing reinforcement between diverging populations. As genetic structure in northern marine and estuarine species may be largely determined by historical glacial cycles of vicariance, contemporary estimates of connectivity should be interpreted in the context of both past and present landscape structure. KEY WORDS:Dispersal · Connectivity · Microsatellite · mtDNA · Glacial isolation · Phylogeography · Rainbow smelt Resale or republication not permitted without written consent of the publisher

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“…Reactions were run on Eppendorf thermocyclers (Eppendorf; http://www.eppendorf.com) and imaged on an FMBioII system (Hitachi Genetic Systems; http://www.miraibo.com). For O. mordax , nine microsatellite loci were used ( Omo 1, Omo 2, Omo 3, Omo 4, Omo 5, Omo 9, Omo 11, Omo 15 and Omo 16) following Coulson et al . (2006).…”

Section: Methodsmentioning

confidence: 99%

Structural and functional connectivity of marine fishes within a semi‐enclosed Newfoundland fjord

Bradbury

Snelgrove

Bentzen

et al. 2009

Journal of Fish Biology

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The interplay between structural connectivity (i.e. habitat continuity) and functional connectivity (i.e. dispersal probability) in marine fishes was examined in a coastal fjord (Holyrood Pond, Newfoundland, Canada) that is completely isolated from the North Atlantic Ocean for most of the year. Genetic differentiation was described in three species (rainbow smelt Osmerus mordax, white hake Urophycis tenuis and Atlantic cod Gadus morhua) with contrasting life histories using seven to 10 microsatellite loci and a protein-coding locus, PanI (G. morhua). Analysis of microsatellite differentiation indicated clear genetic differences between the fjord and coastal regions; however, the magnitude of difference was no more elevated than adjacent bays and was not enhanced by the fjord's isolation. Osmerus mordax was characterized by the highest structure overall with moderate differentiation between the fjord and St Mary's Bay (F(ST)c.0.047). In contrast, U. tenuis and G. morhua displayed weak differentiation (F(ST) < 0.01). Nonetheless, these populations did demonstrate high rates (< 75%) of Bayesian self-assignment. Furthermore, elevated differentiation was observed at the PanI locus in G. morhua between the fjord and other coastal locations. Interestingly, locus-specific genetic differentiation and expected heterozygosity were negatively associated in O. mordax, in contrast to the positive associations observed in U. tenuis and G. morhua. Gene flow in these species is apparently unencumbered by limited structural connectivity, yet the observed differentiation suggests that population structuring exists over small scales despite high dispersal potential.

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Characterization of di‐ and tetranucleotide microsatellite markers in rainbow smelt (Osmerus mordax)

Cited by 14 publications

References 12 publications

Morphological and genetic differentiation in anadromous smelt Osmerus mordax (Mitchill): disentangling the effects of geography and morphology on gene flow

Morphological and genetic differentiation in anadromous smelt Osmerus mordax (Mitchill): disentangling the effects of geography and morphology on gene flow

Contemporary nuclear and mitochondrial genetic clines in a north temperate estuarine fish reflect Pleistocene vicariance

Structural and functional connectivity of marine fishes within a semi‐enclosed Newfoundland fjord

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