Characterization of detergent-soluble proteins of Corynebacterium pseudotuberculosis

Braithwaite, Cleantis; Smith, Elona; Songer, J. Glenn; Reine, Alden H.

doi:10.1016/0378-1135(93)90075-i

Cited by 22 publications

(16 citation statements)

References 20 publications

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“…Some researchers have analyzed and characterized soluble and insoluble proteins that have immunodominant potential [12,79]. Though many other immunogenic excreted-secreted components have been 212 F.A.…”

Section: Identification Of Immunodominant Peptidesmentioning

confidence: 99%

Corynebacterium pseudotuberculosis: microbiology, biochemical properties, pathogenesis and molecular studies of virulence

et al. 2006

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-Corynebacterium pseudotuberculosis is the etiological agent of caseous lymphadenitis (CLA), a common disease in small ruminant populations throughout the world. Once established, this disease is difficult to eradicate because drug therapy is not effective and because the clinical detection of infected animals is of limited efficiency. We reviewed the microbiological, biochemical and taxonomic features of C. pseudotuberculosis, general aspects of infection, the main virulence determinants and currently available commercial vaccines. We also examined the current molecular strategies for the study of virulence in C. pseudotuberculosis, including the latest research on the identification of novel virulence factors and genes, which will help us to better understand the biology of this microorganism. This knowledge may also contribute to the development of improved CLA vaccines, including subunit and DNA-based types, as well as to improve the diagnosis, treatment and control of this disease.

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Section: Identification Of Immunodominant Peptidesmentioning

confidence: 99%

Corynebacterium pseudotuberculosis: microbiology, biochemical properties, pathogenesis and molecular studies of virulence

et al. 2006

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“…A highly significant relationship between the number of mice dying and the haemolytic activity has been demonstrated (23). The SDS-PAGE technique has been successfully employed to characterise protein antigens of C. pseudotuberculosis (2,10,16). Muckle and co-workers (16) showed that antibodies to at least seven antigens of a WC lysate of C. pseudotuberculosis were present in the serum of naturally infected sheep and goats.…”

Section: Discussionmentioning

confidence: 99%

Studies on vaccine development for ovine caseous lymphadenitis

Izgür¹,

Akan²,

Ilhan³

et al. 2010

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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Caseous lymphadenitis (CLA), which is a serious infectious disease of sheep and goats world-wide, has caused significant economic losses in Turkey in recent years. In this study, it was aimed to identify major antigens of Corynebacterium pseudotuberculosis strains isolated in Turkey and to evaluate the efficiency of the vaccine prepared from local strains of C. pseudotuberculosis against bacterial challenge in lambs. C. pseudotuberculosis strains were isolated from six different flocks in Turkey and were classified according to their similarities by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). SDS-PAGE revealed more than 21 bands ranging in size from 15 kDa to 194 kDa present in different concentrations in C. pseudotuberculosis isolates as determined by visual assessment of their approximate molecular masses. Lambs were vaccinated with a bacterin + toxoid + Freund's complete adjuvant (FCA) vaccine, and the vaccine efficiency against bacteriological challenge was evaluated. When a local C. pseudotuberculosis (Pl 18) strain was used and formulated as per ml of the vaccine, 1.5x10 8 cfu of C. pseudotuberculosis bacterin + toxoid + 1/2 FCA of the toxoid, we obtained encouraging results regarding the potential of this vaccine to control of CLA in sheep.

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“…The bacteria in these studies were grown in complex media containing exogenous proteins that would contaminate extractions of extracellular proteins [23, 24]. Studies showed that the use of chemically defined medium (CDM) is an effective strategy to identify bacterial components for therapeutic applications [25].…”

Section: Proteomicsmentioning

confidence: 99%

Progression of ‘Omics’ Methodologies for Understanding the Pathogenicity of Corynebacterium Pseudotuberculosis: The Brazilian Experience

Dorella

Gala-García

Pinto

et al. 2013

Computational and Structural Biotechnology Journal

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Since the first successful attempt at sequencing the Corynebacterium pseudotuberculosis genome, large amounts of genomic, transcriptomic and proteomic data have been generated. C. pseudotuberculosis is an interesting bacterium due to its great zoonotic potential and because it causes considerable economic losses worldwide. Furthermore, different strains of C. pseudotuberculosis are capable of causing various diseases in different hosts. Currently, we seek information about the phylogenetic relationships between different strains of C. pseudotuberculosis isolates from different hosts across the world and to employ these data to develop tools to diagnose and eradicate the diseases these strains cause. In this review, we present the latest findings on C. pseudotuberculosis that have been obtained with the most advanced techniques for sequencing and genomic organization. We also discuss the development of in silico tools for processing these data to prompt a better understanding of this pathogen.

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Characterization of detergent-soluble proteins of Corynebacterium pseudotuberculosis

Cited by 22 publications

References 20 publications

Corynebacterium pseudotuberculosis: microbiology, biochemical properties, pathogenesis and molecular studies of virulence

Corynebacterium pseudotuberculosis: microbiology, biochemical properties, pathogenesis and molecular studies of virulence

Studies on vaccine development for ovine caseous lymphadenitis

Progression of ‘Omics’ Methodologies for Understanding the Pathogenicity of Corynebacterium Pseudotuberculosis: The Brazilian Experience

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