Characterization of dentine structure in three dimensions using FIB‐SEM

Earl, J.S.; Leary, Rowan K.; Perrin, James S.; Brydson, Rik; Harrington, John P.; Markowitz, Kenneth; Milne, S.J.

doi:10.1111/j.1365-2818.2010.03396.x

Cited by 41 publications

(34 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Human teeth, however, are difficult to obtain. Other problems include their small size, variability with age and disease level [9,10,11], infection hazard [12], and ethical issues [13]. Bovine teeth on the other hand are easy to obtain, but have same drawbacks like human teeth, which includes small size, variability, and cross infection.…”

Section: Introductionmentioning

confidence: 99%

Characterization of Dentine to Assess Bond Strength of Dental Composites

et al. 2015

View full text Add to dashboard Cite

This study was performed to develop alternating dentine adhesion models that could help in the evaluation of a self-bonding dental composite. For this purpose dentine from human and ivory was characterized chemically and microscopically before and after acid etching using Raman and SEM. Mechanical properties of dentine were determined using 3 point bend test. Composite bonding to dentine, with and without use of acid pre-treatment and/or the adhesive, were assessed using a shear bond test. Furthermore, micro gap formation after restoration of 3 mm diameter cavities in dentine was assessed by SEM. Initial hydroxyapatite level in ivory was half that in human dentine. Surface hydroxyapatites decreased by approximately half with every 23 s of acid etch. The human dentine strength (56 MPa) was approximately double that of ivory, while the modulus was almost comparable to that of ivory. With adhesive use, average shear bond strengths were 30 and 26 MPa with and without acid etching. With no adhesive, average bond strength was 6 MPa for conventional composites. This, however, increased to 14 MPa with a commercial flowable “self–bonding” composite or upon addition of low levels of an acidic monomer to the experimental composite. The acidic monomer additionally reduced micro-gap formation with the experimental composite. Improved bonding and mechanical properties should reduce composite failures due to recurrent caries or fracture respectively.

show abstract

Section: Introductionmentioning

confidence: 99%

Characterization of Dentine to Assess Bond Strength of Dental Composites

et al. 2015

View full text Add to dashboard Cite

show abstract

“…However, the geometrical nature of these structures must be confirmed through serial sections rather than observed on a single section. In fact, FIB-SEM has been recently employed to assess the three-dimensional aspects of several tissues using a specific imaging software (3,(25)(26)(27), and also in dental histology, where it revealed the connections among dentinal tubules (28). One limitation of this study is that only two-dimensional images were used; that being said, we are currently refining one three-dimensional FIB-SEM reconstruction protocol that will be presented to the bone research community in a near future.…”

Section: Discussionmentioning

confidence: 99%

“…backscattering, a process known as "slice-and-view" (5,6). While targeting specific regions of a given tissue sample can be a challenging task with transmission electron microscopy (TEM), FIB-SEM may overcome such limitation by employing faster visualization of serial ultra-thin section.…”

Section: Introductionmentioning

confidence: 99%

Biological application of focus ion beam-scanning electron microscopy (FIB-SEM) to the imaging of cartilaginous fibrils and osteoblastic cytoplasmic processes

Hasegawa

Endo

Tsuchiya

et al. 2017

Journal of Oral Biosciences

View full text Add to dashboard Cite

Objectives: The aim of this study is a biological application of focused ion beam-scanning electron microscopy (FIB-SEM) to demonstrate serial sectional images of skeletal tissues, here presenting the ultrastructure of 1) cartilaginous extracellular fibrils and 2) osteoblastic cytoplasmic processes.Methods: Seven weeks-old female wild-type mice were fixed with half-Karnovsky solution and subsequent OsO4, and the tibiae were extracted for block staining prior to observation under transmission electron microscope (TEM) and FIB-SEM.Results: TEM showed the fine fibrillar, but somewhat amorphous ultrastructure of the intercolumnar septa in the growth plate cartilage. Alternatively, FIB-SEM revealed bundles of stout fibrils at regular intervals paralleling the septa's longitudinal axis, as well as vesicular structures embedded in the cartilaginous matrix of the proliferative zone. In the primary trabeculae, both TEM and FIB-SEM showed several osteoblastic cytoplasmic processes on the osteoid, with numbers higher than those seen in the bone matrix. FIB-SEM revealed the agglomeration of cytoplasmic processes beneath the osteoblasts, which formed a tubular continuum extending from those cells. Based on these findings, we postulated that osteoblasts not only extend their cytoplasmic processes through to the bone matrix, but also stack these cell processes on the osteoid of the primary trabeculae. Conclusion:Taken together, it is likely that FIB-SEM imaging strategy on serial sections may successfully deliver new insights on the ultrastructure of cartilage and bone tissues. words

show abstract

“…The viability of the SEM technique for the 3D characterization of dentine tubule structure was considered. It has shown the capability to capture the complex structure and interconnection in a porous insulating biological tissue with nanoscale resolution, but over volumes covering thousands of cubic micrometres [14].…”

Section: Discussionmentioning

confidence: 99%

Shear Bond Strength of Ormocer-Based Restorative Material Using Specific and Nonspecific Adhesive Systems

Hamouda

Shehata

2011

ISRN Materials Science

View full text Add to dashboard Cite

The aim of this study was to evaluate the shear bond strength of ormocer-based restorative material bonded to tooth structure using specific ormocer and nonspecific resin-based adhesives. Human molars were prepared to obtain flat buccal enamel surfaces and flat occlusal dentin surfaces. Admira bond, and Prime & Bond NT, Excite, AdheSE, and Prompt-L-Pop were applied to the prepared enamel and dentin surfaces. Ormocer restorative material was inserted into a mold fixed onto the prepared tooth surfaces. The restorative material was applied and cured. The shear bond strength was measured using a universal testing machine. The highest bond strength was recorded for Admira bond. The non-specific adhesives (AdheSE and Prompt-L-Pop) were recorded the lowest bond strength. Etch & Rinse (Prime & Bond NT and Excite) adhesives were recorded an intermediate values. Admira bond usually showed cohesive failure in the material with enamel and cohesive and mixed failure with the dentin. Etch & Rinse adhesives showed mixed failure with the enamel and, mixed and adhesive failures with the dentin. Self-etching adhesives commonly exhibited adhesive mode of failure. So, Etch & Rinse, non-specific bonding agents can be used with Admira ormocer-based restorative material when the specific adhesive is depleted.

show abstract

Characterization of dentine structure in three dimensions using FIB‐SEM

Cited by 41 publications

References 20 publications

Characterization of Dentine to Assess Bond Strength of Dental Composites

Characterization of Dentine to Assess Bond Strength of Dental Composites

Biological application of focus ion beam-scanning electron microscopy (FIB-SEM) to the imaging of cartilaginous fibrils and osteoblastic cytoplasmic processes

Shear Bond Strength of Ormocer-Based Restorative Material Using Specific and Nonspecific Adhesive Systems

Contact Info

Product

Resources

About