Characterization of Dental Pulp Stem Cells of Human Tooth Germs

Abstract

In previous studies, human dental pulp stem cells (hDPSCs) were mainly isolated from adults. In this present study, we characterized hDPSCs isolated from an earlier developmental stage to evaluate the potential usage of these cells for tissue-regenerative therapy. hDPSCs isolated at the crown-completed stage showed a higher proliferation rate than those isolated at a later stage. When the cells from either group were cultured in medium promoting differentiation toward cells of the osteo/odontoblastic lineage, both became alkaline-phosphatase-positive, produced calcified matrix, and were also capable of forming dentin-like matrix on scaffolds in vivo. However, during long-term passage, these cells underwent a change in morphology and lost their differentiation ability. The results of a DNA array experiment showed that the expression of several genes, such as WNT16, was markedly changed with an increasing number of passages, which might have caused the loss of their characteristics as hDPSCs.

Isolation and characterization of stem cells derived from human third molar tooth germs of young adults: implications in neo-vascularization, osteo-, adipo- and neurogenesis

“…Isolation of multipotent MSC-like cells from dental follicles, dental pulp, dental papilla and peri-odontal ligaments, all of which originate from the tooth germ, has been successfully achieved by various groups, including our laboratory. [17][18][19][20]23,24,[26][27][28] Unlike earlier reports, in this study we isolated stem cells for the first time from the entire tooth germ, which includes aforementioned tissues. Although we did not compare side-by-side, this approach yielded higher numbers of cells, showing a higher proliferation rate compared with the dental follicle cells we isolated earlier.…”

“…13 Multipotent tooth germ progenitor cells derived from the dental papilla of developing third molars have been successfully differentiated into ecto-dermal and meso-dermal cells and have shown a therapeutic effect on damaged liver. 27 In a recent study, Takeda et al 28 reported that human dental pulp stem cells isolated from earlier stage (crown-completed) tooth development showed higher proliferation rates than those isolated at a later stage (rootcompleted). However, both cell types showed similar osteo/ odonto-blastic differentiation efficiency.…”

Induced in vitro differentiation of neural-like cells from human exfoliated deciduous teeth-derived stem cells

“…Therefore, there are many interests in banking person-specific tooth-derived stem cells (Arora et al, 2009;Perry et al, 2008). The proliferation rate of SHED is significantly higher than that of DPSCs and BM-MSCs (Nakamura et al, 2009;Takeda et al, 2008). It is interesting to note that DPSC isolation is possible for at least five days after tooth extraction, which implies that immediate processing after extraction may not be necessary for successful DPSC banking.…”

Dental Kök Hücre Kaynaklari Ve Kemi̇k Doku Rejenerasyonunda Kullanilma Potansi̇yelleri̇

“…49 Bir farenin immun sistemi baskılanarak sırtına transplante edilen dental pulpa kök hücrelerinin dentin-pulpa kompleksine benzer bir yapı oluşturduğu izlenmiştir. [50][51][52][53] Düşen süt dişleri, kök hücre çalışmalarında etik açıdan bir sorun olmadan kullanılabilmektedir-ler. 37,38,43,44,[54][55][56] Bu hücrelerin osteoblastlar, odontoblastlar, adipositler, kondrositler, ve hepatositler gibi çeşitli hücre tiplerine (in vitro ve in vivo) diferansiye olma yeteneğine sahip olduğu gösterilmiştir.…”