Characterization of denitrifying polyphosphate-accumulating organisms in activated sludge based on nitrite reductase gene

Tsuneda, Satoshi; Miyauchi, Ryuki; Ohno, Takashi; Hirata, Akira

doi:10.1263/jbb.99.403

Cited by 44 publications

(27 citation statements)

References 15 publications

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“…However, the "Ca. Accumulibacter phosphatis" putative clone was not found in the clone library in our previous study (29). This result is not in conflict with the present study because the "Ca.…”

Section: Discussionsupporting

confidence: 49%

“…Clone Bd was obtained from the sorted cells. Clone 28-8 is from an anaerobic/aerobic SBR (29). Sequences of Sludge Oz, Jazz, and Phrap included in the tree were metagenomic sequences of nirS binned as "Ca.…”

Section: Discussionmentioning

confidence: 99%

“…These observations may support the second explanation. In our previous study (29), the cloning of the nirS gene fragment with the same primer set used in the present study was also performed for similar sludge samples without flow cytometric sorting. As a result, one of the nirS clones occupied approximately 70% of all nirS clones.…”

Section: Discussionmentioning

confidence: 99%

“…The SBR was mixed constantly with a magnetic stirrer (300 rpm) in anaerobic and aerobic phases. Synthetic wastewater of the following composition was used as the feeding solution: 512 mg of CH 3 (29). Acetate has been commonly used for enrichment of PAOs responsible for an enhanced biological phosphorus removal in wastewater treatment plants (25).…”

Section: Methodsmentioning

confidence: 99%

“…Accumulibacter phosphatis" can denitrify or not. An attempt to obtain a better understanding of the population dynamics of denitrifying bacteria in anaerobic/aerobic SBR was made in our previous study (29), but the investigation of the denitrification ability of "Ca. Accumulibacter phosphatis" was not successful.…”

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confidence: 99%

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Diversity of Nitrite Reductase Genes in “ Candidatus Accumulibacter phosphatis”-Dominated Cultures Enriched by Flow-Cytometric Sorting

Miyauchi

Oki

Aoi

et al. 2007

Appl Environ Microbiol

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"Candidatus Accumulibacter phosphatis" is considered a polyphosphate-accumulating organism (PAO) though it has not been isolated yet. To reveal the denitrification ability of this organism, we first concentrated this organism by flow cytometric sorting following fluorescence in situ hybridization (FISH) using specific probes for this organism. The purity of the target cells was about 97% of total cell count in the sorted sample. The PCR amplification of the nitrite reductase genes (nirK and nirS) from unsorted and sorted cells was performed. Although nirK and nirS were amplified from unsorted cells, only nirS was detected from sorted cells, indicating that "Ca. Accumulibacter phosphatis" has nirS. Furthermore, nirS fragments were cloned from unsorted (Ba clone library) and sorted (Bd clone library) cells and classified by restriction fragment length polymorphism analysis. The most dominant clone in clone library Ba, which represented 62% of the total number of clones, was not found in clone library Bd. In contrast, the most dominant clone in clone library Bd, which represented 59% of the total number of clones, represented only 2% of the total number of clones in clone library Ba, indicating that this clone could be that of "Ca. Accumulibacter phosphatis." The sequence of this nirS clone exhibited less than 90% similarity to the sequences of known denitrifying bacteria in the database. The recovery of the nirS genes makes it likely that "Ca. Accumulibacter phosphatis" behaves as a denitrifying PAO capable of utilizing nitrite instead of oxygen as an electron acceptor for phosphorus uptake.

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Section: Discussionsupporting

confidence: 49%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

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Diversity of Nitrite Reductase Genes in “ Candidatus Accumulibacter phosphatis”-Dominated Cultures Enriched by Flow-Cytometric Sorting

Miyauchi

Oki

Aoi

et al. 2007

Appl Environ Microbiol

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Nitrite reductase genes as functional markers to investigate diversity of denitrifying bacteria during agricultural waste composting

Chen

Zhou

et al. 2014

Appl Microbiol Biotechnol

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The purpose of this study was to investigate the diversity of denitrifier community during agricultural waste composting. The diversity and dynamics of the denitrifying genes (nirK and nirS) were determined using polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE). Relationships between physico-chemical parameters and denitrifying genes structures were simultaneously evaluated by redundancy analysis (RDA). Phylogenetic analysis indicated that nirK clones grouped into six clusters and nirS clones into two major clusters, respectively. The results showed a very high diversity of nir gene sequences within composting samples. RDA showed that the nirK and nirS gene structures were significantly related to pH and pile temperature (P < 0.05). Significant amounts of the variation (49.2 and 38.3 % for nirK and nirS genes, respectively) were explained by pH and pile temperature, suggesting that those two parameters were the most likely ones to influence, or be influenced by the denitrifiers harboring nirK and nirS genes.

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Operation performance and microbial community dynamics of phosphorus removal sludge with different electron acceptors

Shao

et al. 2014

Journal of Industrial Microbiology and Biotechnology

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Operation performances of phosphorus removal sludge with different electron acceptors in three parallel SBRs were firstly compared in the present study, and the effect of post-aeration on denitrifying phosphorus removal was also studied. Moreover, community dynamics of different phosphorus removal sludge was systematically investigated with high-throughput sequencing for the first time. TP removal rates for nitrate-, nitrite-, and oxygen-based phosphorus removal sludge were 84.8, 78.5, and 87.4 %, with an average effluent TP concentration of 0.758, 0.931, and 0.632 mg/l. The average specific phosphorus release and uptake rates were 20.3, 10.8, and 21.5, and 9.43, 8.68, and 10.8 mgP/(gVSS h), respectively. Moreover, electron utilization efficiency of denitrifying phosphorus removal sludge with nitrate as electron acceptor was higher than nitrite, with P/e(-) were 2.21 and 1.51 mol-P/mol-e(-), respectively. With the assistance of post-aeration for nitrate-based denitrifying phosphorus removal sludge, settling ability could be improved, with SVI decreased from 120 to 80 and 72 ml/g when post-aeration time was 0, 10, and 30 min, respectively. Moreover, further phosphorus removal could be achieved during post-aeration with increased aeration time. However, the anoxic phosphorus uptake was deteriorated, which was likely a result of shifted microbial community structure. Post-aeration of approximately 10 min was proposed for denitrifying phosphorus removal. Nitrate- and nitrite-based denitrifying phosphorus removal sludge exhibited similar community structure. More phosphorus accumulating organisms were enriched under anaerobic-aerobic conditions, while anaerobic-anoxic conditions were favorable for suppressing glycogen-accumulating organisms. Significant differences in pathogenic bacterial community profiles revealed in the current study indicated the potential public health hazards of non-aeration activated sludge system.

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Characterization of denitrifying polyphosphate-accumulating organisms in activated sludge based on nitrite reductase gene

Cited by 44 publications

References 15 publications

Diversity of Nitrite Reductase Genes in “ Candidatus Accumulibacter phosphatis”-Dominated Cultures Enriched by Flow-Cytometric Sorting

Diversity of Nitrite Reductase Genes in “ Candidatus Accumulibacter phosphatis”-Dominated Cultures Enriched by Flow-Cytometric Sorting

Nitrite reductase genes as functional markers to investigate diversity of denitrifying bacteria during agricultural waste composting

Operation performance and microbial community dynamics of phosphorus removal sludge with different electron acceptors

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