Characterization of defective interfering (DI) particles of Pestedes petitsruminants vaccine virus Sungri/96 strain-implications in vaccine upscaling

Bora, Mousumi; Yousuf, Raja Wasim; Dhar, P.; Manu, M.; Zafir, Insha; Mishra, Bina; Rajak, Kaushal Kishor; Singh, Rabindra Prasad

doi:10.1016/j.biologicals.2019.09.008

Cited by 6 publications

(3 citation statements)

References 24 publications

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“…New sequencing using high-throughput technology of the IC89 strain held at the Pirbright Institute (the origin of the GenBank sequence) would help identify the real nucleotide differences separating the two virus stocks. Furthermore, cell culture passages are capable of generating defective interfering particles that could make PPRV less virulent by preventing replication of the standard virus [ 50 ]. The presence of interfering particles may explain the low virulence observed in the PPRV IC89 strain at our disposal.…”

Section: Discussionmentioning

confidence: 99%

Comparative pathogenesis of peste des petits ruminants virus strains of difference virulence

Eloiflin

Grau‐Roma

Python

et al. 2022

Vet Res

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Peste des petits ruminants (PPR) is an acute disease of small ruminants caused by a morbillivirus. Clinical observation of the disease in the field revealed that several species of small ruminants are affected to varying degrees. This difference in disease-related effects could depend either on the host or on the virulence of the virus strain. A previous study highlighted the difference in virulence between two strains of PPRV used to infect Saanen goats. For this breed, PPRV Morocco 2008 strain (MA08) was highly virulent while PPRV Côte d’Ivoire 1989 (IC89) strain induced mild disease. Experimental studies generally based on healthy and young animals do not permit exploration of the natural variability of the host susceptibility to PPRV. Therefore, building on the previous study on Saanen goats, the current study focussed on this breed of goat and used commercially available animals with an unknown history of infection with other pathogens. Results confirmed the previous disease pattern for PPRV IC89 and MA08 strains. Viral RNA detection, macroscopic and histological lesions were stronger for the highly virulent MA08 strain. We show here for the first time that viral RNA can be detected in the tissues of vaccinated animals. Viral RNA was also detected for the first time in serum samples, which is in agreement with the role of circulating immune cells in transporting the virus into host target organs. Thus, this study provides insight into the pathogenesis of strains of different virulence of PPRV and will help to better understand the onset of the disease.

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Section: Discussionmentioning

confidence: 99%

Comparative pathogenesis of peste des petits ruminants virus strains of difference virulence

Eloiflin

Grau‐Roma

Python

et al. 2022

Vet Res

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“…For FC virus stock, the number of genomic nucleic acid molecules is equal to the number of virus particles (copies/ml = Particles/ml) so the P/I ratio of FC virus can be replaced by full capsid‐to‐infectivity (FC/I) ratio. In the present study, FC/I ratio is defined as

FC / normalI ratio goodbreak= {Log}_{10} ([], ((), copies / ml) / ((), {TCID}_{50} / ml)) .

In general, virus P/I or FC/I ratio changes according to the conditions used for virus amplification and harvest; for example, the MOI is known to impact the P/I ratio 20 . Amplification of MVM after changing the MOI resulted in virus stocks with a different FC/I ratio (Figure 6a).…”

Section: Resultsmentioning

confidence: 99%

“…In the present study, FC/I ratio is defined as

In general, virus P/I or FC/I ratio changes according to the conditions used for virus amplification and harvest; for example, the MOI is known to impact the P/I ratio. 20 Amplification of MVM after changing the MOI resulted in virus stocks with a different FC/I ratio (Figure 6a ).…”

Section: Resultsmentioning

confidence: 99%

Particle‐based analysis elucidates the real retention capacities of virus filters and enables optimal virus clearance study design with evaluation systems of diverse virological characteristics

Kayukawa

Yanagibashi

Hongo‐Hirasaki

et al. 2022

Biotechnology Progress

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In virus clearance study (VCS) design, the amount of virus loaded onto the virus filters (VF) must be carefully controlled. A large amount of virus is required to demonstrate sufficient virus removal capability; however, too high a viral load causes virus breakthrough and reduces log reduction values. We have seen marked variation in the virus removal performance for VFs even with identical VCS design. Understanding how identical virus infectivity, materials and operating conditions can yield such different results is key to optimizing VCS design. The present study developed a particle number‐based method for VCS and investigated the effects on VF performance of discrepancies between apparent virus amount and total particle number of minute virus of mice. Co‐spiking of empty and genome‐containing particles resulted in a decrease in the virus removal performance proportional to the co‐spike ratio. This suggests that empty particles are captured in the same way as genome‐containing particles, competing for retention capacity. In addition, between virus titration methods with about 2.0 Log10 difference in particle‐to‐infectivity ratios, there was a 20‐fold decrease in virus retention capacity limiting the throughput that maintains the required LRV (e.g., 4.0), calculated using infectivity titers. These findings suggest that ignoring virus particle number in VCS design can cause virus overloading and accelerate filter breakthrough. This article asserts the importance of focusing on virus particle number and discusses optimization of VCS design that is unaffected by virological characteristics of evaluation systems and adequately reflect the VF retention capacity.

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Product-Related Impurities in Therapeutic Virus Bioprocessing

2023

Bioprocess and Analytics Development for Virus-Based Advanced Therapeutics and Medicinal Products (ATMPs)

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Characterization of defective interfering (DI) particles of Pestedes petitsruminants vaccine virus Sungri/96 strain-implications in vaccine upscaling

Cited by 6 publications

References 24 publications

Comparative pathogenesis of peste des petits ruminants virus strains of difference virulence

Comparative pathogenesis of peste des petits ruminants virus strains of difference virulence

Particle‐based analysis elucidates the real retention capacities of virus filters and enables optimal virus clearance study design with evaluation systems of diverse virological characteristics

Product-Related Impurities in Therapeutic Virus Bioprocessing

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