Characterization of DEAD-box family of RNA helicases in tomato provides insights into their roles in biotic and abiotic stresses

Pandey, Saurabh; Muthamilarasan, Mehanathan; Sharma, Namisha; Chaudhry, Vaishali; Dulani, Priya; Shweta, Shweta; Jha, Sarita; Mathur, Saloni

doi:10.1016/j.envexpbot.2018.11.018

Cited by 23 publications

(17 citation statements)

References 51 publications

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“…This was also reported as a result of seed priming by Hussain et al, [9] and Alayafi [28]. Pandey and his co-authors [92] found that membrane RNA helicases recognized, contributing to development, and stress tolerance. It is settled from the findings that AsA improved chilling stress tolerance of tomato seedlings concerning transcriptional induction of associated defensive genes, similar to the results of Kosová et al [93] and Park and Park [94].…”

Section: Relative Gene Expressionsupporting

confidence: 60%

Exogenous Ascorbic Acid Induced Chilling Tolerance in Tomato Plants Through Modulating Metabolism, Osmolytes, Antioxidants, and Transcriptional Regulation of Catalase and Heat Shock Proteins

Elkelish

Qari

Mazrou

et al. 2020

Plants

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Chilling, a sort of cold stress, is a typical abiotic ecological stress that impacts the development as well as the growth of crops. The present study was carried to investigate the role of ascorbic acid root priming in enhancing tolerance of tomato seedlings against acute chilling stress. The treatments included untreated control, ascorbic acid-treated plants (AsA; 0.5 mM), acute chilling-stressed plants (4 °C), and chilling stressed seedlings treated by ascorbic acid. Exposure to acute chilling stress reduced growth in terms of length, fresh and dry biomass, pigment synthesis, and photosynthesis. AsA was effective in mitigating the injurious effects of chilling stress to significant levels when supplied at 0.5 mM concentrations. AsA priming reduced the chilling mediated oxidative damage by lowering the electrolyte leakage, lipid peroxidation, and hydrogen peroxide. Moreover, up regulating the activity of enzymatic components of the antioxidant system. Further, 0.5 mM AsA proved beneficial in enhancing ions uptake in normal and chilling stressed seedlings. At the gene expression level, AsA significantly lowered the expression level of CAT and heat shock protein genes. Therefore, we theorize that the implementation of exogenous AsA treatment reduced the negative effects of severe chilling stress on tomato.

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Section: Relative Gene Expressionsupporting

confidence: 60%

Exogenous Ascorbic Acid Induced Chilling Tolerance in Tomato Plants Through Modulating Metabolism, Osmolytes, Antioxidants, and Transcriptional Regulation of Catalase and Heat Shock Proteins

Elkelish

Qari

Mazrou

et al. 2020

Plants

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“…In this analysis, we excluded sequences containing large insertions between the two helicase core domains (DEAD and helicase_c), a specific architecture we exclusively detected in the algae, possibly representing an evolutionary group lost by land plants. Similar to previous studies (Pandey et al 2019 ), the phylogenetic analysis revealed that the DEAD-box RNA helicases cluster into six clades (Fig. 1 b and Supplementary Fig.…”

Section: Resultssupporting

confidence: 87%

“…Sequence similarity searches (pBLAST) using Pp RH1 as query resulted in the identification of 47 P. patens , 28 Marchantia polymorpha, 35 Mesostigma viride and 37 Chlamydomonas reinhardtii RNA helicases containing the DEAD-box motif. The domain structure and sizes of these proteins, in addition to their homologues in Solanum lycopersicum (Pandey et al 2019 ), O. sativa and A. thaliana (Xu et al 2013 ) can be found in Supplementary Table S2. Structural analysis of the selected RNA helicases from these organisms, representing major Viridiplantae groups, revealed that only a restricted number of additional domains, including WW (PF00397), DUF4217 (PF13959), DBP10CT (PF08147), GUCT and Zf_CCHC (PF00098), are found in combination and in an identical arrangement to the helicase core.…”

Section: Resultsmentioning

confidence: 99%

“…Conversely, DEAD-box RNA helicase 42 (OsRH42, LOC_Os08g06344) in O. sativa was shown to be necessary for effective splicing of pre-mRNA during mRNA maturation at low temperatures (Lu et al 2020 ). In two comprehensive expression profiling studies in tomato, organ- and/or tissue-specific expression patterns of DEAD-box RNA helicases were observed in relation to various abiotic (Cai et al 2018 ) and biotic (Pandey et al 2019 ) stress conditions, thus providing evidence for RNA helicases performing diverse roles in cellular stress responses and during plant growth and development.…”

Section: Introductionmentioning

confidence: 99%

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The nuclear GUCT domain-containing DEAD-box RNA helicases govern gametophytic and sporophytic development in Physcomitrium patens

et al. 2021

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Key messageIn Physcomitrium patens, PpRH1/PpRH2 are GUCT-domain-containing DEAD-BOX RNA helicases localize to the nucleus. They are implicated in cell and tissue development in all stages of the moss life cycle. Abstract The DEAD-box-containing RNA helicase family encompasses a large and functionally important group of enzymes involved in cellular processes committed to the metabolism of RNA, including its transcription, processing, transport, translation and decay. Studies indicate this protein family has implied roles in plant vegetative and reproductive developmental processes as well as response to environmental stresses such has cold and high salinity. We focus here on a small conserved sub-group of GUCT domain-containing RNA helicase in the moss Physcomitrium patens. Phylogenetic analysis shows that RNA helicases containing the GUCT domain form a distinct conserved clade across the green lineage. In this clade, the P. patens genome possesses two closely related paralogues RNA helicases predicted to be nuclear, PpRH1 and PpRH2. Using in-locus gene fluorescent tagging we show that PpRH1 is localized to the nucleus in protonema. Analysis of PpRH1 and PpRH2 deletions, individually and together, indicates their potential roles in protonema, gametophore and sporophyte cellular and tissue development in P. patens. Additionally, the ultrastructural analysis of phyllid chloroplasts in Δrh2 and Δrh1/2 shows distinct starch granule accumulation under standard growth conditions associated with changes in photosynthetic activity parameters. We could not detect effects of either temperature or stress on protonema growth or PpRH1 and PpRH2 expression. Together, these results suggest that nuclear GUCT-containing RNA helicases play a role primarily in developmental processes directly or indirectly linked to photosynthesis activity in the moss P. patens.

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“…This RPT4 binds to the intergenic region of the ToLCNDV genome and inhibits the transcription of viral genes by RNA polymerase II (Sahu et al, 2016). Furthermore, transient DEAD35-silencing in a tolerant cultivar results in increased susceptibility to ToLCNDV infection (Pandey et al, 2019). Similarly, Arabidopsis histone reader EML1 (EMSY-LIKE 1) suppresses CabLCV infection by inhibiting the association of RNA polymerase II with viral chromatin and represses viral gene expression (Coursey et al, 2018).…”

Section: Combating Host-mediated Antiviral Strategiesmentioning

confidence: 99%

Plant Antiviral Immunity Against Geminiviruses and Viral Counter-Defense for Survival

Kumar

2019

Front. Microbiol.

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The family Geminiviridae includes plant-infecting viruses whose genomes are composed of one or two circular non-enveloped ssDNAs(+) of about 2.5–5.2 kb each in size. These insect-transmissible geminiviruses cause significant crop losses across continents and pose a serious threat to food security. Under the control of promoters generally located within the intergenic region, their genomes encode five to eight ORFs from overlapping viral transcripts. Most proteins encoded by geminiviruses perform multiple functions, such as suppressing defense responses, hijacking ubiquitin-proteasomal pathways, altering hormonal responses, manipulating cell cycle regulation, and exploiting protein-signaling cascades. Geminiviruses establish complex but coordinated interactions with several host elements to spread and facilitate successful infection cycles. Consequently, plants have evolved several multilayered defense strategies against geminivirus infection and distribution. Recent studies on the evasion of host-mediated resistance factors by various geminivirus proteins through novel mechanisms have provided new insights into the development of antiviral strategies against geminiviruses. This review summarizes the current knowledge concerning virus movement within and between cells, as well as the recent advances in our understanding of the biological roles of virus-encoded proteins in manipulating host-mediated responses and insect transmission. This review also highlights unexplored areas that may increase our understanding of the biology of geminiviruses and how to combat these important plant pathogens.

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Characterization of DEAD-box family of RNA helicases in tomato provides insights into their roles in biotic and abiotic stresses

Cited by 23 publications

References 51 publications

Exogenous Ascorbic Acid Induced Chilling Tolerance in Tomato Plants Through Modulating Metabolism, Osmolytes, Antioxidants, and Transcriptional Regulation of Catalase and Heat Shock Proteins

Exogenous Ascorbic Acid Induced Chilling Tolerance in Tomato Plants Through Modulating Metabolism, Osmolytes, Antioxidants, and Transcriptional Regulation of Catalase and Heat Shock Proteins

The nuclear GUCT domain-containing DEAD-box RNA helicases govern gametophytic and sporophytic development in Physcomitrium patens

Plant Antiviral Immunity Against Geminiviruses and Viral Counter-Defense for Survival

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