Characterization of dark septate endophyte fungi associated with cultivated soybean at two growth stages

Rothen, Carolina Paola; Miranda, Victoria; Aranda-Rickert, Adriana; Fracchia, Sebastián; Rodríguez, Martha

doi:10.1016/j.apsoil.2017.07.033

Cited by 18 publications

(12 citation statements)

References 53 publications

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“…On the other hand Ascomycota is known to include "dark septate endophytes" (DSEs) and has been observed in roots of plants that grow in stressful and nutrient-limited [52] and have shown a positive effect on plant growth [53,54] probably because of nitrogen mineralization and pathogen protection [55].…”

Section: Discussionmentioning

confidence: 99%

Fungal and Bacterial Microbiome Associated with the Rhizosphere of Native Plants from the Atacama Desert

et al. 2020

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The rhizosphere microbiome is key in survival, development, and stress tolerance in plants. Salinity, drought, and extreme temperatures are frequent events in the Atacama Desert, considered the driest in the world. However, little information of the rhizosphere microbiome and its possible contribution to the adaptation and tolerance of plants that inhabit the desert is available. We used a high-throughput Illumina MiSeq sequencing approach to explore the composition, diversity, and functions of fungal and bacterial communities of the rhizosphere of Baccharis scandens and Solanum chilense native plants from the Atacama Desert. Our results showed that the fungal phyla Ascomycota and Basidiomycota and the bacterial phyla Actinobacteria and Proteobacteria were the dominant taxa in the rhizosphere of both plants. The linear discriminant analysis (LDA) effect size (LefSe) of the rhizosphere communities associated with B. scandens showed the genera Penicillium and Arthrobacter were the preferential taxa, whereas the genera Oidiodendron and Nitrospirae was the preferential taxa in S. chilense. Both plant showed similar diversity, richness, and abundance according to Shannon index, observed OTUs, and evenness. Our results indicate that there are no significant differences (p = 0.1) between the fungal and bacterial communities of both plants, however through LefSe, we find taxa associated with each plant species and the PCoA shows a separation between the samples of each species. This study provides knowledge to relate the assembly of the microbiome to the adaptability to drought stress in desert plants.

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Section: Discussionmentioning

confidence: 99%

Fungal and Bacterial Microbiome Associated with the Rhizosphere of Native Plants from the Atacama Desert

et al. 2020

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“…In Argentina, DSE of crops are not well-known yet, their identities are poorly understood, and even less their interaction with cereal species (Rothen et al, 2017). In this work, a strain of a DSE fungus, morphologically identified as M. bolleyi, was isolated from wheat roots in agricultural soils in the province of Buenos Aires.…”

Section: Discussionmentioning

confidence: 99%

“…Although, DSE studies have focused on descriptive analyses of the colonization and fungal strains, mostly isolated from natural ecosystems (Jumpponen & Trappe, 1998;Newsham, 2011), while, when considering crop species, the information related to their association with DSEs is scarce and limited (Wang et al, 2016;Yuan et al, 2010;Rothen et al, 2017;Fernandes et al, 2015;Muthukumar & Tamilselvi, 2010). Argentina produces approximately 2% of the global wheat production, with an average planting area of 6 million hectares in the last 5 years (Andrade & Satorre, 2015), thus becoming one of the most important crops in the country.…”

Section: Introductionmentioning

confidence: 99%

Xylariales): Physiological characterization and structural features of its association with wheat.

Rothen¹,

Miranda²,

Fracchia³

et al. 2018

Bol. Soc. Argent. Bot.

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Summary: Plant roots can be colonized by asymptomatic fungal strains belonging to several taxa, among them, the group defined as Dark Septate Endophytes (DSE). Microdochium bolleyi commonly colonizes wheat roots and other crops. It is considered a weak pathogen or even a non-pathogenic fungal species, which has also been considered as a potential biocontrol agent against aggressive soil-borne pathogens in cereal crops. We isolated a strain of M. bolleyi from wheat roots sampled in a crop field in Argentina, and characterized its abilities to grow in different carbon and nitrogen sources, to produce indole and to solubilize phosphorus; also several enzymatic activities were evaluated. In addition, resynthesis was performed under controlled conditions in order to characterize root fungal colonization under both, optical and transmission microscopy. The strain 22-1 colonized wheat root parenchymal tissue, forming chlamysdospores inside parenchymal cells and root hairs, and poorly grew in carbon and nitrogen sources. This fungus also synthesized indoles in in vitro culture, but it cannot solubilize phosphorus. Only amylase activity was detected out of seven enzymatic activity measured. Microdochium bolleyi (strain 22-1) colonized the roots, it formed typical DSE fungal structures and behaved like a "true endophyte"; however further studies are necessary to elucidate its role in the association with wheat.Key words: Dark septate endophytes, Microdochium bolleyi, root endophytes, wheat crop.Resumen: Microdochium bolleyi (Ascomycota: Xylariales): Caracterización fisiológica y caracteres estructurales de su asociación con trigo. Las raíces de las plantas hospedan una gran diversidad de hongos, entre ellos, se encuentran los Endofitos Septados Oscuros (ESO). Microdochium bolleyi coloniza las raíces de trigo y otros cereales, aunque algunos autores lo han considerado un patógeno débil, otros han demostrado su acción biocontroladora contra patógenos agresivos del suelo. En el presente trabajo, se aisló una cepa de M. bolleyi (22-1) de raíces de trigo. Esta cepa fue metabólicamente caracterizada y se realizó un ensayo de resíntesis bajo condiciones controladas con el fin de caracterizar la colonización del hongo en la raíz bajo microscopía óptica y de transmisión. Su crecimiento fue escaso en las fuentes de carbono y nitrógeno evaluadas, sintetizó indoles en cultivo in vitro, pero no mostró habilidades para solubilizar el fósforo, por último, solo se detectó actividad amilasa. La cepa 22-1 coloniza la corteza radicular del trigo, formando clamidosporas melanizadas inter e intracelularmente y en el interior de los pelos radiculares. Microdochium bolleyi (cepa 22-1) coloniza la raíz de trigo formando las típicas estructuras de los ESO y comportándose como un "verdadero endófito", sin embargo, se necesitan más estudios para terminar de dilucidar su papel en la asociación con el trigo.Palabras clave: Endofitos septados oscuros, hongos endofitos, Microdochium bolleyi, trigo.

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“…In comparison to fungal endophytes of plant aerial portions, the identification of DSE fungi in commercially important crop species is limited. The diversity of root endophytic (DSE) fungus in rare wild rice (Oryza granulate) in China, and soybean roots was studied by Yuan et al (2010) and Rothen et al (2017), respectively. The DSE fungi are polyphyletic and mainly belong to the orders such as Capnodiales, Eurotiales, Helotiales, Hypocreales, Microascales, Pleosporales and Xylariales (Newsham, 2011;Knapp et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Assessment of root colonization, identification, and phosphate solubilization activity of root endophytic fungi obtained from hybrid tomato Lycopersicon esculentum Mill. var. TNAU CO3

Surendirakumar,

Rajendran,

Pandey

et al. 2024

JMycR

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Land plants, in general, rely heavily on positive interactions with fungal endophytes for growth, disease resistance, and stress tolerance. Root colonization, isolation and tri-calcium phosphate (TCP) solubilizing activity of root endophytic fungi (REF) obtained from hybrid tomato (Lycopersicon esculentum Mill. var. TNAU CO3) plants grown in natural fields were examined. All field-collected tomato roots were positively colonized by DSE (dark septate endophyte) fungi. A total of 94 culturable fungal isolates belonging to 8 distinct orders, 9 genera, and 11 species were recovered and identified by morphological examination. In L. esculentum roots, the endophytic fungal colonization rate (CR%) was noticeably high (62.67%). Aspergillus niger was the dominant fungus with a relative abundance (RA%) of 23.4%, while A. niger, Cladosporium cladosporioides, and Fusarium oxysporum had the maximum isolation frequency (IF%). Furthermore, we evaluated the nature of the identified fungal isolates as endophytes by a pot study. Thus, 9 of 11 fungi colonized the test plant roots with clear intracellular structures, including Alternaria spp., C. cladosporioides, Colletotrichum spp., and F. oxysporum, which are DSE fungi. Furthermore, four REF were able to solubilize the TCP, with SI% ranging from 1.3 to 2.4%. This study's findings will help to improve our understanding of REF interactions and their potential ability to solubilize inorganic phosphate, as well as the application of these fungi to increase the development and productivity of diverse agricultural plants in sustainable agriculture.

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Characterization of dark septate endophyte fungi associated with cultivated soybean at two growth stages

Cited by 18 publications

References 53 publications

Fungal and Bacterial Microbiome Associated with the Rhizosphere of Native Plants from the Atacama Desert

Fungal and Bacterial Microbiome Associated with the Rhizosphere of Native Plants from the Atacama Desert

Xylariales): Physiological characterization and structural features of its association with wheat.

Assessment of root colonization, identification, and phosphate solubilization activity of root endophytic fungi obtained from hybrid tomato Lycopersicon esculentum Mill. var. TNAU CO3

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