Characterization of cytotoxic proteins from mistletoe (Viscum album L.)

Jung, Marie‐Louise; Baudino, Sylvie; Ribéreau-Gayon, G.; Beck, Jean‐Paul

doi:10.1016/0304-3835(90)90044-x

Cited by 63 publications

(33 citation statements)

References 13 publications

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“…The extent of cytotoxic damage was highly dependent on the culture medium with which the mistletoe extract was diluted: the presence of fetal calf serum in the medium reduced the cytotoxic effects of Iscador QuFrF. Cytotoxicity of mistletoe is mainly attributed to its lectins and viscotoxins (Hohskog et al, 1988;Jung et al, 1990;Stettin et al, 1990). As mistletoe lectins bind to sugar residues, it seems reasonable to speculate that glycoproteins from serum compete with cell surface glycoproteins for lectin binding, thereby reducing the amount of lectins engulfed by cells.…”

Section: Discussionmentioning

confidence: 95%

Effect of a mistletoe extract (Iscador® QuFrF) on viability and migratory behavior of human peripheral CD4+ and CD8+ T lymphocytes in three-dimensional collagen lattices

Nikolai

Friedl

Werner

et al. 1997

In Vitro Cell.Dev.Biol.-Animal

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Migration and tissue distribution of immunocompetent cells may be critical prerequisites for efficient immune surveillance. The effect of various concentrations of the mistletoe extract Iscador QuFrF on the locomotory behavior and viability of immunomagnetically isolated human CD4+ and CD8+ T lymphocytes within three-dimensional collagen gels was investigated. Although variation in baseline activities of spontaneously migrating T cells was donor-dependent, a dose-dependent stimulation of the locomotory activity in both CD4+ and CD8+ T cells for noncytotoxic concentrations of Iscador QuFrF (0.25-1.25 micrograms/ml) was detected. The optimal concentration of mistletoe extract and time of maximal response were specific for each donor. As shown by cell tracking and subsequent data analysis, CD4+ T cells exposed to the mistletoe extract displayed a significant increase in mean velocity and time locomoting; total distance migrated was nearly doubled. In contrast, CD8+ T cells showed less pronounced changes in these critical parameters. Cytotoxic effects of the mistletoe preparation on T lymphocytes, which could at least partially be attributed to the induction of apoptosis, were drastically reduced in the presence of fetal calf serum in the culture system. Our data suggest that the direct stimulation of T-cell migration in the presence of mistletoe components may modulate in a dose-dependent manner the system of immune surveillance and recognition in patients under mistletoe therapy.

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Section: Discussionmentioning

confidence: 95%

Effect of a mistletoe extract (Iscador® QuFrF) on viability and migratory behavior of human peripheral CD4+ and CD8+ T lymphocytes in three-dimensional collagen lattices

Nikolai

Friedl

Werner

et al. 1997

In Vitro Cell.Dev.Biol.-Animal

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“…The lectins inhibit protein biosynthesis by inactivating the 60S ribosomal subunit (Endo et al, 1988;Witthohn et al, 1996). For all three MLs, cytotoxic effects by inducing apoptosis in a variety of malignant tumour cells have been shown (Schumacher et al, 1995b;Valentiner et al, 2002;Lyu et al, 2002;Yoon et al, 1999), with ML-I being the predominant cytotoxic agent (Jung et al, 1990). In addition to its cytotoxic action, ML-I stimulates the immune system non-specifically by increasing the number and activity of natural killer cells and neutrophils (Beuth et al, 1995).…”

Section: Introductionmentioning

confidence: 97%

Influence of mistletoe lectins and cytokines induced by them on cell proliferation of human melanoma cells in vitro

et al. 2005

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“…Genellikle aktiviteden sorumlu ve çeşitli konakçılar üzerinde yetişen V. album alt türlerinin viskotoksin, fenilpropanoit ve lignan türevi madde leri (12)(13)(14)(15)(16) içerip içermediğini tespit etmek üzere eşit miktarda bitkiden hareketle ve değişik solvanlarla yapılan fraksiyonlama sonucu elde edi- İnce tabaka kromatografısi yöntemiyle standart maddelerle yapılan karşılaştırmalı incelemeler sonucu, çeşitli konakçılardaki örneklerin ver diği lekelerin renk konsantrasyonu ve büyüklüğü, tabloda "+" işaretinin sayısı ile orantılı olarak verilmiştir. V.album ssp.…”

Section: Sonuç Ve Tartişmaunclassified

Farklı Konakçılardaki Vìscum album L. Örneklerinin Etken Maddeleri Yönünden İncelenmesi : Studies on the Active Principles of the Various Viscum album L. Collected From Different Host Plants.

Ergün¹,

Deliorman²

1996

Ankara Universitesi Eczacilik Fakultesi Dergisi

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ÖZETBu çalışmada, çeşitli konakçı bitkilerden toplanan farklı Viscum album L. (Loranthaceae) örneklerinin kimyasal bileşimi; temel etken maddeleri açısından karşılaştırılmıştır. Nitel analizler sonucu alkaloit, fenilpropanoit, flavonoit, kardiyoaktif hcterozit, lignan, redüktör ve nonredüktör oz, saponozit, siyanogenetik heterozit, tanen ve viskotoksin gibi etken madde gruplarının varlığı tespit edilmiştir.Anahtar Kelimeler: Loranthaceae, Viscum album, konakçı bitki, etken madde. SUMMARYIn this study, chemical composition of the different Viscum album L. (Loranthaceae) samples collected from various host plants are compared from the view point of main active principles. Qualitative analysis revea led that alkaloid, phenylpropanoid, flavonoid, cardioactive glycoside, lig nan, reducing or nonreducing sugar, saponin, cyanogenic glycoside, tan nin and viscotoxin are found as active principle in the drug.

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Characterization of cytotoxic proteins from mistletoe (Viscum album L.)

Cited by 63 publications

References 13 publications

Effect of a mistletoe extract (Iscador® QuFrF) on viability and migratory behavior of human peripheral CD4+ and CD8+ T lymphocytes in three-dimensional collagen lattices

Effect of a mistletoe extract (Iscador® QuFrF) on viability and migratory behavior of human peripheral CD4+ and CD8+ T lymphocytes in three-dimensional collagen lattices

Influence of mistletoe lectins and cytokines induced by them on cell proliferation of human melanoma cells in vitro

Farklı Konakçılardaki Vìscum album L. Örneklerinin Etken Maddeleri Yönünden İncelenmesi : Studies on the Active Principles of the Various Viscum album L. Collected From Different Host Plants.

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