Characterization of cytotoxic compound from marine sediment derived actinomycete Streptomyces avidinii strain SU4

Sudha, S.; Selvam, M. Masilamani

doi:10.1016/s2221-1691(12)60227-5

Cited by 41 publications

(31 citation statements)

References 16 publications

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“…These findings indicate that endophytic Actinomycetes are reservoirs of various bioactive metabolites that may serve as potential anticancer/antimicrobial drugs for patients in critical health conditions (ICU), immunocompromised patients (such as cancer patients) and those with multidrug MRSA infections. The results of this work are consistent with the data on the antitumor activities of bioactive metabolites derived from marine Actinomycetes such as Streptomyces avidinii strain SU4, reported by Sudha and Selvam (2012).…”

Section: Anticancer Activity Of Compounds a And Bsupporting

confidence: 91%

“…The MS ion source temperature was 200EC, interface temperature was 240EC, event time 0.5 s, solvent cut time 5 min and MS start 5 min, MS end time 35 min. The mass spectra were recorded in electron ionization (EI) mode at 70ev as previously described by Sudha and Selvam (2012). Chromatogram integration and library searches were performed using the libraries of the Central Laboratory of Pesticides, Agricultural Research Center, Egypt.…”

Section: Gas Chromatography/mass Spectrometry Analysis (Gc/ms) Of Thementioning

confidence: 99%

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Production of bioactive metabolites from different marine endophytic Streptomyces species and testing them against methicillin-resistant Staphylococcus aureus (MRSA) and cancer cell lines

El-Gendy¹,

Kamel²,

Hekal³

et al. 2018

bta

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The aim of the work was to identify Actinomycetes strains that are able to produce high levels of anti-MRSA metabolites with a potential use in curing resistant Staphylococcus aureus infections. Twenty-six endophytic Actinomycetes strains were isolated from various marine invertebrates living in two different marine ecosystems (the Mediterranean Sea and the Red Sea). Among them, Streptomyces sp. RS-9 (obtained from the soft coral Sarcophyton sp. from the Red Sea) and Streptomyces sp. MS-26 (obtained from the jellyfish derived from the Mediterranean Sea) demonstrated the highest antagonistic activity against 18 isolates of methicillin-resistant Staphylococcus aureus (MRSA) and 2 isolates of methicillin-resistant coagulase-negative Staphylococci (MRCoNS). The optimization of culture conditions, which includes the time course (at the 6 th and the 3 rd day of growth), the incubation temperature (27.5EC), pH (8.0 and 6.0), nutritional requirements comprising different metals, carbon (glucose and glycerol, respectively), and nitrogen (mixture of valine with peanut and a mixture of soybean with methionine and asparagines, respectively) sources for RS-9 and MS-26 strains, respectively, resulted in a 2.2-fold and 2.5-fold increase in the productivity of anti-MRSA metabolites. The GC/MS analysis proved the presence of different bioactive compounds in Streptomyces sp. RS-9 and Streptomyces sp. MS-26 extracts. Two pure compounds, A) 1-Methyl-2-acetyl-6-acetoxy-7-methoxy-(1,2,3,4-tetrahydroisoquinoline) and B)-4-(dimethylamino)-1,4,4d,5,12,12a-hexahydro-3,10,11,12a-tetrahydroxy-6-methyl-1,12-dioxo-,[4S-(4-α,4a,al]-(2-naphthacene carboxamide, which is a well-known antibiotic called anhydrotetracycline), were isolated from Streptomyces sp. MS-26. Those compounds possessed potent anti-MRSA activity against all the tested clinical MRSA and MRCoNS isolates. The minimum inhibition concentration (MIC) of the above two compounds ranged from 16 to 1024 μgml !1 and 4 to 128 μgml !1 , respectively. The minimum bactericidal concentration (MBC) ranged from 16 to 1024 μgml !1 and 8 to 128 μgml !1 , respectively with anticancer activity against colon cancer (HCT-16), liver cancer (HepG-2), and lung cancer (A-549) cell lines.

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Section: Anticancer Activity Of Compounds a And Bsupporting

confidence: 91%

Section: Gas Chromatography/mass Spectrometry Analysis (Gc/ms) Of Thementioning

confidence: 99%

Production of bioactive metabolites from different marine endophytic Streptomyces species and testing them against methicillin-resistant Staphylococcus aureus (MRSA) and cancer cell lines

El-Gendy¹,

Kamel²,

Hekal³

et al. 2018

bta

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“…Because the criteria of the American National Cancer Institute consider IC 50 values of < 30 μg/mL indicative of a promising extract [19], F2-5 and F3-2-9 can be considered as potential sources of anticancer drugs. However, whether EtOAc extracts of A. marina leaves contain other secondary metabolites, such as alkaloids, triterpenoids, and tannins, requires confirmation in further studies.…”

Section: Discussionmentioning

confidence: 99%

“…DNA fragmentation assays were performed as previously described [19]. Briefly, cells were incubated with reagents at various concentrations for 48 h and were then lysed using lysis buffer.…”

Section: Methodsmentioning

confidence: 99%

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Polyphenol-rich Avicennia marina leaf extracts induce apoptosis in human breast and liver cancer cells and in a nude mouse xenograft model

Huang

et al. 2016

Oncotarget

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Avicennia marina is the most abundant and common mangrove species and has been used as a traditional medicine for skin diseases, rheumatism, ulcers, and smallpox. However, its anticancer activities and polyphenol contents remain poorly characterized. Thus, here we investigated anticancer activities of secondary A. marina metabolites that were purified by sequential soxhlet extraction in water, ethanol, methanol, and ethyl acetate (EtOAc). Experiments were performed in three human breast cancer cell lines (AU565, MDA-MB-231, and BT483), two human liver cancer cell lines (HepG2 and Huh7), and one normal cell line (NIH3T3). The chemotherapeutic potential of A. marina extracts was evaluated in a xenograft mouse model. The present data show that EtOAc extracts of A. marina leaves have the highest phenolic and flavonoid contents and anticancer activities and, following column chromatography, the EtOAc fractions F2-5, F3-2-9, and F3-2-10 showed higher cytotoxic effects than the other fractions. 1H-NMR and 13C-NMR profiles indicated that the F3-2-10 fraction contained avicennones D and E. EtOAc extracts of A. marina leaves also suppressed xenograft MDA-MB-231 tumor growth in nude mice, suggesting that EtOAc extracts of A. marina leaves may provide a useful treatment for breast cancer.

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Bioprospecting in Cuatro Cienegas: A Set of Open Possibilities to Save the Ecosystem

Arocha-Garza

Avilés-Arnaut

Medina-Chávez

et al. 2018

Cuatro Ciénegas Basin: An Endangered Hyperdiverse Oasis

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Characterization of cytotoxic compound from marine sediment derived actinomycete Streptomyces avidinii strain SU4

Cited by 41 publications

References 16 publications

Production of bioactive metabolites from different marine endophytic Streptomyces species and testing them against methicillin-resistant Staphylococcus aureus (MRSA) and cancer cell lines

Production of bioactive metabolites from different marine endophytic Streptomyces species and testing them against methicillin-resistant Staphylococcus aureus (MRSA) and cancer cell lines

Polyphenol-rich Avicennia marina leaf extracts induce apoptosis in human breast and liver cancer cells and in a nude mouse xenograft model

Bioprospecting in Cuatro Cienegas: A Set of Open Possibilities to Save the Ecosystem

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