Characterization of cytoplasmic male sterility of rice with Lead Rice cytoplasm in comparison with that with Chinsurah Boro II cytoplasm

Itabashi, Etsuko; Kazama, Tomohiko; Toriyama, Kinya

doi:10.1007/s00299-008-0625-7

Cited by 41 publications

(55 citation statements)

References 15 publications

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“…We suggest that the different RNA binding activity observed in this study may be due to the differences between the intragenic sequences of atp6-orf79 and atp6-orfH79. In LD-CMS rice, LD-atp6-orf79 is an orthologous gene associated with CMS, yet restoration can occur with either Rf1 or Rf2 (Itabashi et al, 2009). Rf2 encodes a GRP with a mitochondrial presequence, suggesting that a divergent restoration pathway may exist even within the same CMS system.…”

Section: A Coordinated Model Of the Functions Of Rf5 And Grp In Hl-cmmentioning

confidence: 99%

The Rice Pentatricopeptide Repeat Protein RF5 Restores Fertility in Hong-Lian Cytoplasmic Male-Sterile Lines via a Complex with the Glycine-Rich Protein GRP162

et al. 2012

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The cytoplasmic male sterility (CMS) phenotype in plants can be reversed by the action of nuclear-encoded fertility restorer (Rf) genes. The molecular mechanism involved in Rf gene-mediated processing of CMS-associated transcripts is unclear, as are the identities of other proteins that may be involved in the CMS-Rf interaction. In this study, we cloned the restorer gene Rf5 for Hong-Lian CMS in rice and studied its fertility restoration mechanism with respect to the processing of the CMS-associated transcript atp6-orfH79. RF5, a pentatricopeptide repeat (PPR) protein, was unable to bind to this CMSassociated transcript; however, a partner protein of RF5 (GRP162, a Gly-rich protein encoding 162 amino acids) was identified to bind to atp6-orfH79. GRP162 was found to physically interact with RF5 and to bind to atp6-orfH79 via an RNA recognition motif. Furthermore, we found that RF5 and GRP162 are both components of a restoration of fertility complex (RFC) that is 400 to 500 kD in size and can cleave CMS-associated transcripts in vitro. Evidence that a PPR protein interacts directly with a Gly-rich protein to form a subunit of the RFC provides a new perspective on the molecular mechanisms underlying fertility restoration.

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Section: A Coordinated Model Of the Functions Of Rf5 And Grp In Hl-cmmentioning

confidence: 99%

The Rice Pentatricopeptide Repeat Protein RF5 Restores Fertility in Hong-Lian Cytoplasmic Male-Sterile Lines via a Complex with the Glycine-Rich Protein GRP162

et al. 2012

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“…In rice, ORF79 is a stable membrane-integrated polypeptide expressed in all tissues. In a line of CMS rice carrying a variant of orf79, termed L-orf79, the L-ORF79 polypeptide is not detected [100]. Itabashi et al [100] suggested that this might be due to a sequence alteration in the 5 untranslated region that prevents translation.…”

Section: Why Do Most Of the Unique Orfs Not Matter In Angiosperm Mitomentioning

confidence: 99%

“…In a line of CMS rice carrying a variant of orf79, termed L-orf79, the L-ORF79 polypeptide is not detected [100]. Itabashi et al [100] suggested that this might be due to a sequence alteration in the 5 untranslated region that prevents translation. However, it is also possible that a Glu to Ala substitution in the coding region may alter the higher-order structure, making L-ORF79 accessible to the degradation system.…”

Section: Why Do Most Of the Unique Orfs Not Matter In Angiosperm Mitomentioning

confidence: 99%

Cost of Having the Largest Mitochondrial Genome: Evolutionary Mechanism of Plant Mitochondrial Genome

Kitazaki

Kubo

2010

Journal of Botany

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The angiosperm mitochondrial genome is the largest and least gene-dense among the eukaryotes, because its intergenic regions are expanded. There seems to be no functional constraint on the size of the intergenic regions; angiosperms maintain the large mitochondrial genome size by a currently unknown mechanism. After a brief description of the angiosperm mitochondrial genome, this review focuses on our current knowledge of the mechanisms that control the maintenance and alteration of the genome. In both processes, the control of homologous recombination is crucial in terms of site and frequency. The copy numbers of various types of mitochondrial DNA molecules may also be controlled, especially during transmission of the mitochondrial genome from one generation to the next. An important characteristic of angiosperm mitochondria is that they contain polypeptides that are translated from open reading frames created as byproducts of genome alteration and that are generally nonfunctional. Such polypeptides have potential to evolve into functional ones responsible for mitochondrially encoded traits such as cytoplasmic male sterility or may be remnants of the former functional polypeptides.

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“…BT-CMS originated from the indica variety of Chinsurah boro II ( Shinjyo 1975 ) and has been studied most extensively in rice ( Iwabuchi et al 1993, Kazama and Toriyama 2003, Wang et al 2006. The LD-CMS line originated from the Burnese cultivar Lead Rice ( Watanabe 1971, Itabashi et al 2009 ). As a result, we found that the OsNek3 mRNA expression level was extensively reduced in the CW-CMS line as in our previous microarray ( Fig.…”

Section: Osnek3 Mrna Expresssion Is Suppressed Under a Cms Backgroundmentioning

confidence: 99%

Cytoplasmic Male Sterility-Related Protein Kinase, OsNek3, is Regulated Downstream of Mitochondrial Protein Phosphatase 2C, DCW11

Fujii

Yamada

Toriyama

2009

Plant and Cell Physiology

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OsNek3 ( Oryza sativa L. NIMA-related kinase) and DCW11 encoding a mitochondrial putative protein phosphatase 2C were found in our previous microarray study as downregulated genes in the rice CW-CMS line, which lacked pollen germination ability. Further analysis of DCW11 revealed that DCW11 is strongly correlated with CW-CMS occurrence. Here we show the relationship between OsNek3 and DCW11. OsNek3 was preferentially expressed in mature pollen. A knockout mutant with Tos17 inserted into OsNek3 did not show any pollen-defective phenotype. On the other hand, plants overexpressing OsNek3 occasionally produced a peculiar pollen structure in which the outer cell wall of four pollen grains fused together even at the mature pollen stages, which resembled that of quartet mutants in Arabidopsis . OsNek3 was shown to interact with a LIM domain-containing protein, OsPLIM2b, whose expression was strongly specifi c in mature pollen, suggesting that OsNek3 might play a role in pollen germination. OsNek3 was shown to be down-regulated in DCW11-knockdown lines, whereas osnek3 mutation did not result in DCW11 down-regulation. These results suggest that OsNek3 is downstream of DCW11 in retrograde signaling from the mitochondria to the nucleus and is involved in CW-CMS.

show abstract

Characterization of cytoplasmic male sterility of rice with Lead Rice cytoplasm in comparison with that with Chinsurah Boro II cytoplasm

Cited by 41 publications

References 15 publications

The Rice Pentatricopeptide Repeat Protein RF5 Restores Fertility in Hong-Lian Cytoplasmic Male-Sterile Lines via a Complex with the Glycine-Rich Protein GRP162

The Rice Pentatricopeptide Repeat Protein RF5 Restores Fertility in Hong-Lian Cytoplasmic Male-Sterile Lines via a Complex with the Glycine-Rich Protein GRP162

Cost of Having the Largest Mitochondrial Genome: Evolutionary Mechanism of Plant Mitochondrial Genome

Cytoplasmic Male Sterility-Related Protein Kinase, OsNek3, is Regulated Downstream of Mitochondrial Protein Phosphatase 2C, DCW11

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