Characterization of culture supernatant proteins from Brucella abortus and its protection effects against murine brucellosis

Lee, Jin Ju; Lim, Jeong Ju; Kim, Dae Geun; Simborio, Hannah Leah Tadeja; Kim, Dong Hyeok; Reyes, Alisha Wehdnesday Bernardo; Min, Wongi; Lee, Hu Jang; Kim, Dong Hee; Chang, Hong; Kim, Suk

doi:10.1016/j.cimid.2014.06.001

Cited by 20 publications

(15 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…More than twenty-seven proteins including CuZn SOD, OMPs, GroEL and DnaK were identified (Figure 1). Immunization of mice with culture supernatant proteins induced a strong humoral and cell mediated immune responses and exhibited higher protection upon B. abortus infection [28].…”

Section: Brucella Exoproteomementioning

confidence: 99%

Proteomics of Brucella

Poetsch

Marchesini

2020

Proteomes

View full text Add to dashboard Cite

Brucella spp. are Gram negative intracellular bacteria responsible for brucellosis, a worldwide distributed zoonosis. A prominent aspect of the Brucella life cycle is its ability to invade, survive and multiply within host cells. Comprehensive approaches, such as proteomics, have aided in unravelling the molecular mechanisms underlying Brucella pathogenesis. Technological and methodological advancements such as increased instrument performance and multiplexed quantification have broadened the range of proteome studies, enabling new and improved analyses, providing deeper and more accurate proteome coverage. Indeed, proteomics has demonstrated its contribution to key research questions in Brucella biology, i.e., immunodominant proteins, host-cell interaction, stress response, antibiotic targets and resistance, protein secretion. Here, we review the proteomics of Brucella with a focus on more recent works and novel findings, ranging from reconfiguration of the intracellular bacterial proteome and studies on proteomic profiles of Brucella infected tissues, to the identification of Brucella extracellular proteins with putative roles in cell signaling and pathogenesis. In conclusion, proteomics has yielded copious new candidates and hypotheses that require future verification. It is expected that proteomics will continue to be an invaluable tool for Brucella and applications will further extend to the currently ill-explored aspects including, among others, protein processing and post-translational modification.

show abstract

Section: Brucella Exoproteomementioning

confidence: 99%

Proteomics of Brucella

Poetsch

Marchesini

2020

Proteomes

View full text Add to dashboard Cite

show abstract

“…B. abortus strain 544 (ATCC23448) was used as the virulent challenge strain [ 7 12 ]. The bacterial strains, plasmids, and primers used in this study are listed in Table 1 .…”

Section: Methodsmentioning

confidence: 99%

Vaccination of goats with a combinationSalmonellavector expressing fourBrucellaantigens (BLS, PrpA, Omp19, and SOD) confers protection againstBrucella abortusinfection

et al. 2018

View full text Add to dashboard Cite

Salmonella is an intracellular pathogen with a cellular infection mechanism similar to that of Brucella, making it a suitable choice for use in an anti-Brucella immune boost system. This study explores the efficacy of a Salmonella Typhimurium delivery-based combination vaccine for four heterologous Brucella antigens (Brucella lumazine synthase, proline racemase subunit A, outer-membrane protein 19, and Cu/Zn superoxide dismutase) targeting brucellosis in goats. We inoculated the attenuated Salmonella delivery-based vaccine combination subcutaneously at two different inoculation levels; 5 × 109 colony-forming unit (CFU)/mL (Group B) and 5 × 1010 CFU/mL (Group C) and challenged the inoculations with virulent Brucella abortus at 6 weeks post-immunization. Serum immunoglobulin G titers against individual antigens in Salmonella immunized goats (Group C) were significantly higher than those of the non-immunized goats (Group A) at 3 and 6 weeks after vaccination. Upon antigenic stimulation, interferon-γ from peripheral blood mononuclear cells was significantly elevated in Groups B and C compared to that in Group A. The immunized goats had a significantly higher level of protection as demonstrated by the low bacterial loads in most tissues from the goats challenged with B. abortus. Relative real-time polymerase chain reaction results revealed that the expression of Brucella antigens was lower in spleen, kidney, and lung of immunized goats than of non-immunized animals. Also, treatment with our combination vaccine ameliorated histopathological lesions induced by the Brucella infection. Overall, the Salmonella Typhimurium delivery-based combination vaccine was effective in delivering immunogenic Brucella proteins, making it potentially useful in protecting livestock from brucellosis.

show abstract

“…In addition, attenuated S. Typhimurium delivery strain containing pMMP65 vector only, was used as vector control strain [ 21 ]. B. abortus strain 544 was used as the virulent challenge strain [ 23 ]. The recombinant BCSP31, Omp3b, and SOD proteins were overexpressed and purified from HJL906, HJL904, and HJL908 strains, respectively [ 21 ].…”

Section: Methodsmentioning

confidence: 99%

Protective efficacy of a canine brucellosis vaccine candidate based on live attenuated <i>Salmonella</i> expressing recombinant <i>Brucella</i> BCSP31, Omp3b and SOD proteins in Beagles

Kim

Moon

Cho

et al. 2018

The Journal of Veterinary Medical Science

View full text Add to dashboard Cite

The aim of this study was to establish a proof-of-concept of protective efficacy of Salmonella-based B. abortus vaccine candidate in Beagles. Group A Beagles (n=10) were subcutaneously (SC) inoculated with S. Typhimurium delivery strain containing pMMP65 (vector to deliver antigens) only as vector control. Group B Beagles (n=10) were SC vaccinated with the mixture of the three Salmonella delivery strains expressing the recombinant B. abortus BCSP31, Outer membrane protein 3b (Omp3b), and superoxide dismutase (SOD) proteins, respectively. No Salmonella delivery strains were isolated from all tissues tested. Serum IgG, interleukin-4, tumor necrosis factor-alpha, and interferon-gamma concentrations were significantly higher in group B than in group A. Following intraconjunctival challenge with B. abortus 544, among 5 group B Beagles, the challenge strain was isolated from mandibular, and retropharyngeal lymph nodes of three Beagles, and no isolates were observed from all tissues of two Beagle. However, the challenge strains were detected from spleen, uterus (except two Beagles), and mandibular, prescapular, retropharyngeal, and superficial inguinal lymph nodes of all group A Beagles. These results suggest that the mixture of three S. Typhimurium delivery strains be a good vaccine candidate against brucellosis by B. abortus in dogs. Further investigations are needed to improve the protective efficacy of the Salmonella-based B. abortus vaccine candidate and explore its practical application in dogs.

show abstract

Characterization of culture supernatant proteins from Brucella abortus and its protection effects against murine brucellosis

Cited by 20 publications

References 25 publications

Proteomics of Brucella

Proteomics of Brucella

Vaccination of goats with a combinationSalmonellavector expressing fourBrucellaantigens (BLS, PrpA, Omp19, and SOD) confers protection againstBrucella abortusinfection

Protective efficacy of a canine brucellosis vaccine candidate based on live attenuated <i>Salmonella</i> expressing recombinant <i>Brucella</i> BCSP31, Omp3b and SOD proteins in Beagles

Contact Info

Product

Resources

About