Characterization of culturable bacteria from pulp and paper industry wastewater, with the potential for degradation of cellulose, starch, and lipids

Abstract

The search for microbial enzymatic activities applied to wastewater treatment is an important task in environmental biotechnology. Microbial enzymes have been previously explored in hostile habitats. They are increasingly important in extreme habitats; biological wastewater from the pulp and paper mill industry can harbor microorganisms with valuable enzymatic capabilities that can improve the efficiency for the same process of depuration. This study was performed to characterize and evaluate cellulolytic, amylolytic, and lipolytic activities of bacteria isolated from a pulp and paper effluent. The enzymatic activities were evaluated by the formation of a clear halo around the colonies in defined substrate media. By the use of a sequence analysis of 16S rDNA libraries, isolates were identified. The 16S rDNA libraries belong to the Bacillus subtilis, B. megaterium, B. licheniformis, B. pumilus, B. thuringiensis, B. cereus, Chryseobacterium daecheongense, and Microbacterium sediminis (an alkali-tolerant bacteria which has only been isolated from deep-sea sediment). B. cereus was the best strain for cellulose and lipase activities; moreover, C. daecheongense was best for amylase activity. The present study shows that the aerated lagoons from the pulp and paper industry are a promising source of bacterial with different enzyme activities. This data is relevant for industrial applications.

Biodegradation of Marine Pollutants by Microorganisms: A Bibliometric Analysis

“…Accordingly, with 601 frequencies in 2015, the keyword Biodegradation was the highest, and then with a frequency of 492 in 2016, the keyword Bioremediation was determined. Moreover, keywords, such as Gene sequence 30 , Gasoline 31 , Microflora 32 , Wastewater treatment [33][34][35] , and Microbiota 36 in 2019 were identified with frequencies of 36, 32, 27, 26, and 25, respectively.…”

Isolasi dan Karakterisasi Bakteri Selulolitik pada Pakan Fermentasi Eceng Gondok, Tongkol Jagung, dan Bekatul Padi

“…Hal ini diperkuat dengan Nik Shawn et al, (2010) yang melakukan penelitian dengan mengisolasi bakteri penghasil selulosa dari mangrove di beberapa daerah di Fhilipina dan ditemukan salah satu strain bakterinya merupakan Bacillus pumilus. Selain itu Bailon-Salas et al, (2018) menemukan adanya bakteri Bacillus pumilus pada air limbah dari pabrik pembuatan kertas yang dibuktikan melalui identifikasi dari hasil sekuen 16S rDNA.…”

Archives of Environmental Protection

“…In lagoon treatment system, a mixture of Bacillus subtilis, B. megaterium, B. licheniformis, B. pumilus, B. thuringiensis, B. cereus, Chryseobacterium daecheongense, and Microbacterium sediminis were identified in the treated pulp and paper mill wastewater (PPMW), whereas B. cereus was the best strain for cellulose and lipase activities (Bailon--Salas et al 2018). Likewise, Bacillus aryabhattai isolated from PPMW had the ability to reduce color and lignin (Zainith et al 2019).…”