Characterization of Cultivable Bacteria from Brazilian Sponges

Santos-Gandelman, Juliana F.; Santos, O.C.S.; Pontes, Paula Veronesi Marinho; Andrade, Cleyton Lage; Korenblum, Elisa; Muricy, Guilherme; Giambiagi-deMarval, Márcia; Laport, Marinella Silva

doi:10.1007/s10126-013-9518-z

Cited by 14 publications

(10 citation statements)

References 28 publications

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“…The antibiotic resistance profile of the culturable bacteria associated with P. citrina has already been reported. 6 This conforms to the data reported herein, as we have reported genes for quinolone and erythromycin resistance. Besides, the results also indicate that the hologenome of P. citrina contains genes encoding antibiotic resistance to erythromycin, methicillin, mupirocin, quinolone, and tetracycline.…”

Section: Resultssupporting

confidence: 92%

“…The bacterial strains were isolated and identified from P. citrina by Santos-Gandelman and colleagues in an earlier study. 6 Of them, six were selected according to their antibacterial activity against certain medically important strains 7 and/or antibiotic resistance profile. 6 Bacillus Pc31 and Pc32, Enterococcus Pc5b and Shigella Pc5a strains were grown in brain–heart infusion medium (BHI) (Difco, MI, USA), and Bacillus Pc3M and Halomonas Pc51M were grown in a marine medium (Marine 2216, Difco), at 25 °C for 24 h.…”

Section: Methodsmentioning

confidence: 99%

“… * Antibiotic resistance to: aztreonam (ATM R ), ceftazidime (CAZ R ), cephalexin (CFE R ), ciprofloxacin (CIP R ), gentamicin (GEN R ), trimethoprim/sulfamethoxazole (SXT R ), tetracycline (TET R ) (Santos-Gandelman et al . 6 ). …”

Section: Tablementioning

confidence: 99%

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Antibiotic resistance genes detected in the marine sponge Petromica citrina from Brazilian coast

Laport

Pontes

Santos

et al. 2016

Brazilian Journal of Microbiology

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Although antibiotic-resistant pathogens pose a significant threat to human health, the environmental reservoirs of the resistance determinants are still poorly understood. This study reports the detection of resistance genes (ermB, mecA, mupA, qnrA, qnrB and tetL) to antibiotics among certain culturable and unculturable bacteria associated with the marine sponge Petromica citrina. The antimicrobial activities elicited by P. citrina and its associated bacteria are also described. The results indicate that the marine environment could play an important role in the development of antibiotic resistance and the dissemination of resistance genes among bacteria.

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Section: Resultssupporting

confidence: 92%

Section: Methodsmentioning

confidence: 99%

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Antibiotic resistance genes detected in the marine sponge Petromica citrina from Brazilian coast

Laport

Pontes

Santos

et al. 2016

Brazilian Journal of Microbiology

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“…Resistance genes were shown to be present (Durso et al 2012 ) and expressed (Versluis et al 2015 ) in all biological niches with complex microbial communities that so far have been investigated. It is therefore hypothesised that also sponge-associated bacteria harbour resistance genes that could (eventually) become available to human pathogens (Santos-Gandelman et al 2013 ). The bacteria isolated in this study show resistance to a diverse range of antibiotics that are routinely used in the clinic.…”

Section: Discussionmentioning

confidence: 99%

Recovery of Previously Uncultured Bacterial Genera from Three Mediterranean Sponges

et al. 2017

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Sponges often harbour a dense and diverse microbial community. Presently, a large discrepancy exists between the cultivable bacterial fraction from sponges and the community in its natural environment. Here, we aimed to acquire additional insights into cultivability of (previously uncultured) bacteria from three sponge species, namely Aplysina aerophoba, Corticium candelabrum and Petrosia ficiformis, by studying bacterial growth on five media in the form of 60 communities scraped from plates without antibiotics, as well as in the form of individual isolates that were grown on these media supplemented with antibiotics. We applied (double-)barcoded 16S ribosomal RNA (rRNA) gene amplicon sequencing for species identification. We show that previously uncultured bacteria can be cultivated using conventional plating and that application of antibiotics in the media can serve to capture a greater bacterial diversity. Moreover, we present criteria to address an important caveat of the plate scraping method whereby bacteria may be detected that did not actually grow. Fourteen out of 27 cultivated novel taxa (<95% identity of the 16S rRNA gene amplicon to reported species) belong to Actinobacteria, which indicates the presence of a large untapped reservoir of bioactive compounds. Three Flavobacteriaceae spp. were isolated that potentially constitute two new genera and one new species.Electronic supplementary materialThe online version of this article (doi:10.1007/s10126-017-9766-4) contains supplementary material, which is available to authorized users.

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“…Atualmente, membros dos filos Actinobacteria, Bacteroidetes, Cianobacteria, Firmicutes, Planctomycetes, Proteobacteria e Verrucomicrobia têm sido isolados de diversas espécies de esponjas em todo o mundo JACKSON et al, 2012;JUNG et al, 2014;LAVY et al, 2014;MONTALVO et al, 2014;HARDOIM et al, 2015;ESTEVES et al, 2016). Contudo, em especial no Brasil, poucos estudos têm sido direcionados a isolar e caracterizar bactérias associadas a animais marinhos (MENEZES et al, 2010;SANTOS-GANDELMAN et al, 2013;SANTOS et al, 2010LAPORT et al, 2016 MENEZES et al, 2010;TABARES et al, 2011;SUN et al, 2015;SILVA, 2015 a essas mesmas espécies de esponjas amostradas em diferentes locais (POPPELL et al, 2013;GLOECKNER et al, 2014;RUA et al, 2015). Xanthomonadaceae.…”

Section: Introductionunclassified

Exploração da diversidade bacteriana de esponjas marinhas por abordagens dependente e independente de cultivo

Souza¹

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Bioprospecting the bacterial diversity of marine sponges by culture-dependent and culture-independent approaches This study describes the diversity of associated bacterial communities to five marine sponges, and the potential of these microorganisms as producers of bioactive substances with fungicidal properties. Sponges live in symbiosis with microorganisms that have a high ecological interest, evolutionary and biotechnological. However, this microbial system remains poorly understood. To fully understand sponge biology, it is necessary to describe the ecological and evolutionary factors that influence the structure and dynamics of their microbial communities. In this work, it is supported the hypothesis that the taxonomic composition and structure of bacterial communities correlate with phylogenetic relatedness of their corresponding hosts. Bacterial communities associated with the sponges Aplysina fulva, Aiolochroia crassa, Chondrosia collectrix, Didiscus oxeata and Scopalina ruetzleri were examined using the Ion Torrent platform for partial sequencing of the 16S rRNA gene. Seawater surrounding specimens were collected for comparisons. The analysis detected a complex and specific microbial system living in sponges, with the operational taxonomic units dominant classified in the phyla: Acidobacteria, Actinobacteria, Chloroflexi, Proteobacteria and Gemmatimonadetes. Despite sympatric occurrence of the specimens, the studied sponges presented different bacterial compositions that differed from those observed in seawater. However, lower dissimilarities in bacterial communities were clearly observed within sponges from the same phylogenetic group (A. fulva and A. crassa). Isolation of bacteria was done from the sponges D. oxeata and S. ruetzleri. Fifty-six strains were isolated and classified into three phyla: Actinobacteria, Proteobacteria and Firmicutes. Phylogenetic analysis indicated five possible novel bacterial species. Based in a polyphasic taxonomy approach, one of the isolates denominated ASPSP 40 was identified as belonging to a novel species of the genus Saccharopolyspora for which the name, Saccharopolyspora spongiae sp. nov. has been proposed. All bacterial isolates were evaluated by their antagonisms against Pythium species. Two of them, Terrabacter sp. ASPSP 140 and Bacillus sp. ASPSP 434 demonstrated strong potential in inhibiting the following species P. aphanidermatum, P. ultimum and P. graminicola. The bioactive secondary metabolites of both, characterized by LC-MS/MS, were identified as a mixture of cyclic dipepitides belonging to the class of diketopiperazine (DKP). This is the first report of fungicidal activity, and thus the detection of DKP of the genus Terrabacter.

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Characterization of Cultivable Bacteria from Brazilian Sponges

Cited by 14 publications

References 28 publications

Antibiotic resistance genes detected in the marine sponge Petromica citrina from Brazilian coast

Antibiotic resistance genes detected in the marine sponge Petromica citrina from Brazilian coast

Recovery of Previously Uncultured Bacterial Genera from Three Mediterranean Sponges

Exploração da diversidade bacteriana de esponjas marinhas por abordagens dependente e independente de cultivo

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