Characterization of CTX-M and SHV extended-spectrum  -lactamases and associated resistance genes in Escherichia coli strains of food samples in Tunisia

Jouini, Ahlem; Vinué, Laura; Slama, Karim Ben; Sáenz, Yolanda; Klibi, Naouel; Hammami, Salah; Boudabous, Abdellatif; Torres, Carmén

doi:10.1093/jac/dkm316

Cited by 179 publications

(154 citation statements)

References 10 publications

(8 reference statements)

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“…In this study, a single isolate producing ESBL from poultry origin, was identifi ed. In Tunisia, the presence of isolates producing ESBL was reported for the fi rst time by [34], from poultry meat, by using a selective protocol for the isolation of these isolates. However [35], did not identify ESBL-producing isolates from 164 isolates by using a non-selective protocol.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial Resistance in Escherichia coli Isolates from Healthy Poultry, Bovine and Ovine in Tunisia: A Real Animal and Human Health Threat

Abbassi¹

2017

J Clin Microbiol Biochem Technol

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A total of 174 E. coli isolates collected from healthy poultry, bovine and ovine recovered between December 2009 and June 2013 in different geographic location in Tunisia, were assessed and examinated for resistance to antimicrobial agents. Avian isolates showed the highest rates of antibiotic resistance: tetracycline (74.7 %), trimethoprim/sulfamethoxazole and amoxicillin with the same rate of resistance (57 %). Prevalences of resistance to the same four antimicrobials in bovine isolates were 33.3 %, 65 %, 30 %, 28.3 %, respectively. However, for ovine isolates, low resistance rates were observed, except for tetracycline (40 %) and amoxicillin (22.85 %). Only one ESBL-producing isolate from chicken was detected. In addition, seventy-seven (44.2 %) isolates were resistant to three or more classes of antibiotics and were considered multidrug resistant (MDR). Interestingly, avian E. coli isolates were more resistant than bovine and ovine ones. These results provide novel insights into the epidemiological characteristics of poultry, bovine and ovine E.coli isolates in Tunisia, and suggest the need for the prudent use of antimicrobial agents in husbandry and the urgent need to establish a national antibiotic resistance monitoring program.

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Section: Discussionmentioning

confidence: 99%

Antimicrobial Resistance in Escherichia coli Isolates from Healthy Poultry, Bovine and Ovine in Tunisia: A Real Animal and Human Health Threat

Abbassi¹

2017

J Clin Microbiol Biochem Technol

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“…In the ESBL-containing E. coli isolates, the presence of genes encoding TEM, SHV, OXA, CTX-M, and CMY b-lactamases was studied by PCR using primers and conditions previously reported upon and sequencing (Jouini et al, 2007). DNA sequences were compared with those included in the GenBank database as well as with those deposited at the website (http://www.lahey.org/Studies/), in order to determine the specific type of b-lactamase gene.…”

Section: Methodsmentioning

confidence: 99%

Molecular characterization of vancomycin-resistant enterococci and extended-spectrum β-lactamase-containingEscherichia coliisolates in wild birds from the Azores Archipelago

et al. 2011

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To study the prevalence of vancomycin-resistant enterococci (VRE) and extended-spectrum b-lactamase (ESBL)-containing Escherichia coli isolates, and the mechanisms of resistance implicated, 220 faecal samples from wild birds were collected between 2006 and 2010 in the Azores Archipelago. Samples were spread on SlanetzÁBartley agar plates supplemented with 4 mg/l vancomycin and on Levine agar plates supplemented with 2 mg/l cefotaxime for VRE and ESBL-containing E. coli isolation, respectively. vanA-containing enterococcal isolates (four Enterococcus faecium and two Enterococcus durans) and vanC-1 Enterococcus gallinarum isolates were detected in six and seven faecal samples, respectively. VRE isolates showed ampicillin (n 011), ciprofloxacin (n 09), tetracycline (n06), erythromycin (n 05), quinupristin/dalfopristin (n 03) and high-level kanamycin resistance (n 01). The tet(L) and/or tet(M) gene was found in all tetracycline-resistant isolates and the erm(B) gene in all erythromycin-resistant isolates. Three vanAcontaining E. faecium and two E. gallinarum presented specific sequences of the Tn5397 transposon. Four VRE isolates harboured the ace virulence gene. One faecal sample revealed one ESBL-containing E. coli isolate that belongs to the A phylogenetic group, showed a phenotype of resistance to b-lactams and tetracycline, and harboured the bla CTX-M-14 , bla SHV-12 and the tet(A) genes. To our knowledge, this is the first study to focus on defining the prevalence of VRE and/or ESBL-containing E. coli strains in wild birds from the Azores. The data recovered are essential to improve knowledge about the dissemination of resistant strains through wild ecosystems and their possible implications by transferring these resistances to other animals or to humans.

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“…Randomly choosing a colony from each cloacal sample, bla CTX-M (CTX-M groups 1, 8, 9, and 10), bla TEM, bla OXA-1, bla FOX, bla CMY, bla SHV , and ampC beta lactamase genes were studied using PCR (7,8). All obtained amplicons were sequenced on both strands, and sequences were compared with those included in the GenBank database and on the website http:/www.lahey.org/Studies/ to identify the betalactamase genes.…”

Section: Characterization Of Esbl Genesmentioning

confidence: 99%

Extended-spectrum β-lactamases among cloacal Escherichia coliisolates in healthy broilers in Turkey

Ünal¹,

Karagöz²,

Aşkar³

et al. 2017

Turk J Vet Anim Sci

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IntroductionEscherichia coli strains are important pathogens in animals and humans, and are normal commensals in intestinal tracts. The β-lactam group of antibiotics has been intensely used in human and veterinary medicine as infectious disease treatments (1). Extended-spectrum betalactamases (ESBLs) hydrolyze oxyimino-cephalosporins and give resistance to bacteria against the penicillins, cephalosporins (first, second, and third generations), and aztreonam. They are repressed by β-lactamase inhibitors. ESBLs are classified as β-lactamases containing three main families: TEM (named after the patient Temoneria), SHV (sulfhydryl reagent variable), and CTX-M (active on cefotaxime, first isolated in Munich) (2). The prevalence of CTX-M-type β-lactamase-producing E. coli has increased in poultry over the last decade (3).Increasing prevalence of ESBL-producing E. coli in poultry has become a major zoonotic risk because of possible transmission from broilers to humans (3). In addition, ESBL-producing E. coli strains that may have multidrug resistance (MDR) and cause therapeutic problems have been reported in human and veterinary practice (4). The alarming spread of ESBL-producing Enterobacteriaceae poses a serious public health threat and has attracted the attention of scientists, politicians, and the general public globally. There was no previous study about the presence, prevalence, and molecular epidemiology of ESBL-producing E. coli isolates from broilers in Turkey. The aim of the present study was to investigate the resistance phenotype and genotyping characteristics of ESBL-carrying E. coli strains in healthy broilers in Turkey. Materials and methods Samples, isolations, and identificationFrom January to April 2012, 300 samples were collected from cloacal samples of healthy slaughterhouse broilers in two regions of Turkey. Broiler cloacal samples were obtained from a total of 30 different poultry houses

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Characterization of CTX-M and SHV extended-spectrum -lactamases and associated resistance genes in Escherichia coli strains of food samples in Tunisia

Cited by 179 publications

References 10 publications

Antimicrobial Resistance in Escherichia coli Isolates from Healthy Poultry, Bovine and Ovine in Tunisia: A Real Animal and Human Health Threat

Antimicrobial Resistance in Escherichia coli Isolates from Healthy Poultry, Bovine and Ovine in Tunisia: A Real Animal and Human Health Threat

Molecular characterization of vancomycin-resistant enterococci and extended-spectrum β-lactamase-containingEscherichia coliisolates in wild birds from the Azores Archipelago

Extended-spectrum β-lactamases among cloacal Escherichia coliisolates in healthy broilers in Turkey

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