Abstract:Heterologous expression of eukaryotic gene clusters in yeast has been widely used for producing high-value chemicals and bioactive secondary metabolites. However, eukaryotic transcription cis-elements are still under-characterized, and the cross-species expression mechanism remains poorly understood. Here we used the whole expression unit (including original promoter, terminator and open reading frame with introns) of orotidine 5'-monophosphate decarboxylases from fourteen Penicillium species as a showcase, an… Show more
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