Characterization of CRISPR array in Salmonella enterica from asymptomatic people and patients

Zhang, Kai; Zhang, Yue; Wang, Zhenyu; Li, Yang; Xu, Haiyan; Jiao, Xinan; Li, Qiuchun

doi:10.1016/j.ijfoodmicro.2021.109338

Cited by 6 publications

(3 citation statements)

References 33 publications

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“…Dublin recovered from humans and animals ( 41 ). CT identified 76 types with a discriminatory power of 97.6% among 180 clinical Salmonella strains isolated during 2017–2018 ( 42 ). Several previous studies have proven the efficiency of CRISPR-MVLST in typing different Salmonella serovars, including Typhimurium, Newport, and Enteritidis, suggesting the use of this method to complement and validate results obtained by PFGE ( 43 – 46 ).…”

Section: Discussionmentioning

confidence: 99%

Comprehensive Assessment of Subtyping Methods for Improved Surveillance of Foodborne Salmonella

et al. 2022

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In public health field, high-resolution and efficient typing of the bacterial pathogen is essential, considering source-tracking and risk assessment are fundamental issues. Currently, there are no recommendations for applying molecular characterization methods for Salmonella along the food chain, and a systematic in-field investigation comparing subtyping methods in the context of routine surveillance was partially addressed.

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Section: Discussionmentioning

confidence: 99%

Comprehensive Assessment of Subtyping Methods for Improved Surveillance of Foodborne Salmonella

et al. 2022

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“…Both humans and animals (including swine, cattle, chickens, dogs, cats, ornamental birds, reptiles, amphibians, and rodents) can become asymptomatic carriers following Salmonella infection. Serovars Typhimurium, Derby, London, Newport, and Senftenberg commonly cause asymptomatic infections in humans [ 7 , 53 ]. Asymptomatic carriers have the potential to contaminate the environment, water sources, and food by intermittently shedding bacteria in feces over extended periods, thereby posing infection risks to vulnerable populations in the community [ 54 , 55 ].…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial resistance and genetic relatedness of Salmonella serotypes isolated from food, asymptomatic carriers, and clinical cases in Shiyan, China

Lv,

Geng,

et al. 2024

PLoS ONE

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Salmonella is a primary cause of foodborne diseases globally. Despite food contamination and clinical infections garnering substantial attention and research, asymptomatic Salmonella carriers, potential sources of infection, have been comparatively overlooked. In this study, we conducted a comparative analysis of serotype distribution, antimicrobial resistance phenotypes, and genetic profiles of archived Salmonella strains isolated from food (26), asymptomatic carriers (41), and clinical cases (47) in Shiyan City, China. Among the 114 Salmonella strains identified, representing 31 serotypes and 34 Sequence Types (STs), the most prevalent serovars included Typhimurium, Derby, Enteritidis, Thompson, and London, with the most predominant STs being ST11, ST40, ST26, ST34, and ST155. Antimicrobial resistance testing revealed that all strains were only sensitive to meropenem, with 74.6% showing antimicrobial resistance (AMR) and 53.5% demonstrating multidrug resistance (MDR). Strains resistant to five and six classes of antibiotics were the most common. Pearson’s chi-square test showed no statistically significant difference in the occurrence of AMR (p = 0.105) or MDR (p = 0.326) among Salmonella isolates from the three sources. Our findings underscore associations and diversities among Salmonella strains isolated from food, asymptomatic carriers, and clinical patients, emphasizing the need for increased vigilance towards asymptomatic Salmonella carriers by authorities.

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“…With the rapid development of molecular biology, there are various methods for Salmonella subtyping. CRISPR is one of the subtyping methods [ 13 , 15 , 35 ]. The RNA secondary structure of typical DRs were predicted using RNAfold and CRISPRCasFinder, and DRs and spacer sequences were displayed.…”

Section: Discussionmentioning

confidence: 99%

The CRISPR genotypes and genetic diversity of different serogroups of nontyphoidal Salmonella in Guizhou Province, 2013–2018

Bai

Li³

et al. 2022

PLoS ONE

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Nontyphoidal Salmonella is a bacterial and foodborne pathogen that poses a severe public health threat. However, the genetic diversity of different serogroups of nontyphoidal Salmonella in Guizhou is unknown. This study aimed to obtain the RNA secondary structure of the typical direct repeat sequences, the characteristics of clustered regularly interspaced short palindromic repeats (CRISPR) genotypes, and the genetic diversity of different serogroups of nontyphoidal Salmonella strains. The 342 nontyphoidal Salmonella strains were collected from nine cities (prefectures) of Guizhou province during 2013–2018, serotyped by slide agglutination, and examined the molecular genotypes by CRISPR method. The strains were divided into five serogroups. The dominant serogroup was group B (47.08%), followed by group D1 (36.55%). One hundred and thirty-five CRISPR genotypes were detected with 108 novel spacer sequences amongst 981 unique spacer sequences. The diversity of nontyphoidal Salmonella CRISPR loci was not only the deletion, duplication, or point mutation of spacer sequences but also the acquisition of new spacer sequences to form novel genotypes. The CRISPR genotyping was an effective typing method that could reveal the genetic diversity of different nontyphoidal Salmonella serotypes except for S. Enteritidis.

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Characterization of CRISPR array in Salmonella enterica from asymptomatic people and patients

Cited by 6 publications

References 33 publications

Comprehensive Assessment of Subtyping Methods for Improved Surveillance of Foodborne Salmonella

Comprehensive Assessment of Subtyping Methods for Improved Surveillance of Foodborne Salmonella

Antimicrobial resistance and genetic relatedness of Salmonella serotypes isolated from food, asymptomatic carriers, and clinical cases in Shiyan, China

The CRISPR genotypes and genetic diversity of different serogroups of nontyphoidal Salmonella in Guizhou Province, 2013–2018

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