Characterization of contractile 5-hydroxytryptamine receptor subtypes in the in situ autoperfused kidney in the anaesthetized rat

Morán, Asunción; Urbina, Ana-Vega Ortiz de; Martín, Manuel Ángel Franco; García, Miguel Angel Sendín; Rodríguez-Barbero, Alicia; Dorado, Fernando; Román, Luís San

doi:10.1016/j.ejphar.2008.06.106

Cited by 17 publications

(30 citation statements)

References 29 publications

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“…5-HT showed a slight inhibition the electrically-induced PP increases, while α-methyl-5-HT did not modify them. These results are in agreement with those obtained previously by us [20][21][22]24], where 5-HT 2 receptor activation was involved in vasoconstrictor actions of renal vasculature. 5-HT 2 receptors are generally considered as "sympathoexcitatory" [14,27,28]; however, our research team has demonstrated that the vasoconstrictor effect by 5-HT 2 in renal territory is mediated by the renin- angiotensin system [20].…”

Section: Hemodynamic Effects Produced By the Different Treatmentssupporting

confidence: 95%

“…pretreatment significantly increased SBP and PP in all cases (see Table 1); i.a. injection of 5-HT or α-methyl-5-HT (0.0125 μg/kg each) increased PP (11.2 ± 1.7 and 16.5 ± 1.4, respectively) that immediately returned to baseline levels [20][21][22]24]. .…”

Section: Data Presentation and Statistical Evaluationmentioning

confidence: 95%

“…Blood was pumped from the right carotid artery to an aortic pouch from which the left renal artery was the only outlet [19,21,22]. The distal portion of the external circuit was connected to a pressure transducer connected to an e-corder 410 amplifier (Model ED410, Cibertec, Spain) for measurement of the PP.…”

Section: General Methodsmentioning

confidence: 99%

“…The dose of each antagonist was selected after consideration of our previous experience [13,[21][22][23].…”

Section: Other Procedures Applying To All Protocolsmentioning

confidence: 99%

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5-HT1D receptor inhibits renal sympathetic neurotransmission by nitric oxide pathway in anesthetized rats

García-Pedraza

García

Martín

et al. 2015

Vascular Pharmacology

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Section: Hemodynamic Effects Produced By the Different Treatmentssupporting

confidence: 95%

Section: Data Presentation and Statistical Evaluationmentioning

confidence: 95%

Section: General Methodsmentioning

confidence: 99%

“…The dose of each antagonist was selected after consideration of our previous experience [13,[21][22][23].…”

Section: Other Procedures Applying To All Protocolsmentioning

confidence: 99%

See 2 more Smart Citations

5-HT1D receptor inhibits renal sympathetic neurotransmission by nitric oxide pathway in anesthetized rats

García-Pedraza

García

Martín

et al. 2015

Vascular Pharmacology

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“…We opted to study the putative interaction in the well-validated [11], and in our previous experiments with lerimazoline already used rat aorta preparation, having in mind the recognized variability of agonist-induced actions in different vascular beds of rats, which depend on concentrations tested, type of receptor involved, basal vascular tone, vascular bed analyzed and the presence of possible pathological processes [121314]. …”

Section: Introductionmentioning

confidence: 99%

Elucidation of the profound antagonism of contractile action of phenylephrine in rat aorta effected by an atypical sympathomimetic decongestant

Rizvić

Janković

Savić

2017

Korean J Physiol Pharmacol

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Vasoconstrictive properties of sympathomimetic drugs are the basis of their widespread use as decongestants and possible source of adverse responses. Insufficiently substantiated practice of combining decongestants in some marketed preparations, such are those containing phenylephrine and lerimazoline, may affect the overall contractile activity, and thus their therapeutic utility. This study aimed to examine the interaction between lerimazoline and phenylephrine in isolated rat aortic rings, and also to assess the substrate of the obtained lerimazoline-induced attenuation of phenylephrine contraction. Namely, while lower concentrations of lerimazoline (10−6 M and especially 10−7 M) expectedly tended to potentiate the phenylephrine-induced contractions, lerimazoline in higher concentrations (10−4 M and above) unexpectedly and profoundly depleted the phenylephrine concentration-response curve. Suppression of NO with NO synthase (NOS) inhibitor Nw-nitro-L-arginine methyl ester (L-NAME; 10−4 M) or NO scavanger OHB12 (10−3 M), as well as non-specific inhibition of K+-channels with tetraethylammonium (TEA; 10−3 M), have reversed lerimazoline-induced relaxation of phenylephrine contractions, while cyclooxygenase inhibitor indomethacin (10−5 M) did not affect the interaction between two vasoconstrictors. At the receptor level, non-selective 5-HT receptor antagonist methiothepin reversed the attenuating effect of lerimazoline on phenylephrine contraction when applied at 3×10−7 and 10−6 M, but not at the highest concentration (10−4 M). Neither the 5-HT1D-receptor selective antagonist BRL 15572 (10−6 M) nor 5-HT7 receptor selective antagonist SB 269970 (10−6 M) affected the lerimazoline-induced attenuation of phenylephrine activity. The mechanism of lerimazoline-induced suppression of phenylephrine contractions may involve potentiation of activity of NO and K+-channels and activation of some methiothepin-sensitive receptors, possibly of the 5-HT2B subtype.

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Serotonin 5HT2A receptor antagonism mediated anti-inflammatory and anti-fibrotic effect in adriamycin-induced CKD in rats

Kaur

Krishan

2020

Naunyn-Schmiedeberg's Arch Pharmacol

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Characterization of contractile 5-hydroxytryptamine receptor subtypes in the in situ autoperfused kidney in the anaesthetized rat

Cited by 17 publications

References 29 publications

5-HT1D receptor inhibits renal sympathetic neurotransmission by nitric oxide pathway in anesthetized rats

5-HT1D receptor inhibits renal sympathetic neurotransmission by nitric oxide pathway in anesthetized rats

Elucidation of the profound antagonism of contractile action of phenylephrine in rat aorta effected by an atypical sympathomimetic decongestant

Serotonin 5HT2A receptor antagonism mediated anti-inflammatory and anti-fibrotic effect in adriamycin-induced CKD in rats

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