Characterization of constituents in Stellera chamaejasme L. by rapid‐resolution liquid chromatography–diode array detection and electrospray ionization time‐of‐flight mass spectrometry

Abstract

A reliable and rapid method based on rapid-resolution liquid chromatography-diode array detection (RRLC-DAD) and electrospray ionization time-of-flight mass spectrometry (ESI-TOF/MS) has been developed for the isolation and characterization of multiple constituents in the root of Stellera chamaejasme L., which was extracted by sonication with methanol in an optimized procedure. Separation of the multiple constituents was achieved on an Agilent Zorbax XDB-C18 (50x3.0 mm i.d.; 1.8 microm) column using a gradient elution at a flow rate of 0.4 mL/min. The detection wavelength was 210 nm. Mass spectra were acquired in both positive and negative modes. A formula database of the known chemical constituents in the root of Stellera chamaejasme L. was established by an Agilent software. Twenty-two obvious peaks appeared in the total ion chromatogram and nine of them were characterized by TOF/MS. The RRLC-DAD and ESI-TOF/MS method with ultrasonic extraction would be useful for rapid and effective characterization of chemical constituents in the root of Stellera chamaejasme L.

Ultra‐high performance liquid chromatography with linear ion trap‐Orbitrap hybrid mass spectrometry combined with a systematic strategy based on fragment ions for the rapid separation and characterization of components in Stellera chamaejasme extracts

“…However, due to the intrinsic complexity of the chemical constitutes of S. chamaejasme, hardly any investigations formed an overall recognition for the chemical profiles of this herb but remained in the stage of separation and confirmation for several or a class of compounds. Zhao et al [5] detected 22 isolated chromatography peaks in the total ion current chromatogram (TIC) and nine of them were characterized by RPLC-DAD tandem ESI-Q-TOF-MS. And because of the unavailability of commercial reference standards and the lack of multi-grade fragmentation ability of Q-TOF instrument, diterpenes could not be identified and the reliability of identification results which only based on the accurate molecular weights was doubtable in some degree. Su et al [6] characterized and compared the chemical constitutes of three Chinese herbs "Langdu" (Stellera chamaejasme, Euphorbia fischeriana, and Euphorbia ebracteolata) by LC-MS/MS analysis, but this investigation mainly focused on developing the analytical strategy in the light of respective makers for differentiating these three herbs from the related species.…”

The determination of flavonoids in Wikstroemia indica C. A. Mey. by liquid chromatography with photo‐diode array detection and negative electrospray ionization tandem mass spectrometry

“…(b) to (e) each contained a deprotonated molecule at m/z 541 in the full scan mass spectra. The root of Stellera chamaejasme , part of the same family as W. indica , has been reported to contain wikstrol A and its isomers chamaejasmine, chamaechromone, neochamaejasmin, and isochamaejasmin . These isomers may be related to the compounds with the same deprotonated species at m/z 541.…”

Determination of chamaechromone in rat plasma by liquid chromatography–tandem mass spectrometry: Application to pharmacokinetic study

“…About 200 L blood samples were collected by scissoring rat tails into heparinized tubes. This was performed at 0 (pre-dose), 0.5, 1,2,4,7,8,9,10,11,12,14,16,20,24,36,48 and 60 h after administration orally and at 0 (pre-dose), 0.083, 0.17, 0.25, 0.5, 1, 1.5, 2, 3, 5, 7, 9, 12, 24 and 48 h after i.v. administration via the tail vein.…”

“…Most of them were based on liquid chromatography-mass spectrometry (LC-MS) [8], rapid-resolution liquid chromatography-diode array detection (RRLC-DAD) and electrospray ionization time-of-flight mass spectrometry (ESI-TOF/MS) [10], high performance liquid chromatography with ultraviolet detection (HPLC-UV) [11], highresolution ESMS [11] and NMR spectrometry [11,12] for detection and identification of the constituents. Until now, no method has been reported on pharmacokinetic study.…”