Characterization of conjunctival microbiome dysbiosis associated with allergic conjunctivitis

Liang, Qiaoxing; Li, Jing; Zhang, Shiyao; Liao, Yinglin; Guo, Shixin; Liang, Juanran; Deng, Xiaoyi; Liu, Yu; Zou, Bin; Wen, Xiaofeng; Liang, Lingyi; Wei, Lai

doi:10.22541/au.159715010.01330710

Cited by 2 publications

(3 citation statements)

References 49 publications

(56 reference statements)

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“…In VKC, Bacteroidetes and Fusobacteria constitute the core bacterial microbiome, which includes different species of gram‐negative bacteria, whereas the fungal microbiome analysis revealed increased abundance of Malasseziaceae family members 91 . In another study, SAC/PAC patients exhibited a significantly different microbiome as compared to VKC, with Brevibacterium aurantiacum and Staphylococcus sciuri species more predominant in SAC/PAC and Streptococcus species in VKC 93 …”

Section: Factors Contributing To Ocular Barrier Dysregulation In Oamentioning

confidence: 94%

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Epithelial barrier dysfunction in ocular allergy

Singh

Diebold

Sahu

et al. 2021

Allergy

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The epithelial barrier is the first line of defense that forms a protective barrier against pathogens, pollutants, and allergens. Epithelial barrier dysfunction has been recently implicated in the development of allergic diseases such as asthma, atopic dermatitis, food allergy, and rhinitis. However, there is limited knowledge on epithelial barrier dysfunction in ocular allergy (OA). Since the ocular surface is directly exposed to the environment, it is important to understand the role of ocular epithelia and their dysfunction in OA. Impaired epithelial barrier enhances allergen uptake, which lead to activation of immune responses and development of chronic inflammation as seen in allergies. Abnormal expression of tight junction proteins that helps to maintain epithelial integrity has been reported in OA but sufficient data not available in chronic atopic (AKC) and vernal keratoconjunctivitis (VKC), the pathophysiology of which is not just complex, but also the current treatments are not completely effective. This review provides an overview of studies, which indicates the role of barrier dysfunction in OA, and highlights how ocular barrier dysfunction possibly contributes to the disease pathogenesis. The review also explores the potential of ocular epithelial barrier repair strategies as preventive and therapeutic approach.

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Section: Factors Contributing To Ocular Barrier Dysregulation In Oamentioning

confidence: 94%

“…91 In another study, SAC/PAC patients exhibited a significantly different microbiome as compared to VKC, with Brevibacterium aurantiacum and Staphylococcus sciuri species more predominant in SAC/ PAC and Streptococcus species in VKC. 93…”

Section: Early-life Events Microbiome and Epithelial Barrier Disruptionmentioning

confidence: 99%

Epithelial barrier dysfunction in ocular allergy

Singh

Diebold

Sahu

et al. 2021

Allergy

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“…The DNA metagenomic shotgun sequencing of the stool samples was performed as previously described. 17 In brief, the VAHTS Universal DNA library Prep Kit for Illumina (Vazyme, Nanjing, China) was used to prepare sequencing libraries and the KAPA SYBR FAST qPCR Kit (Kapa Biosystems, Wilmington, MA, USA) was used to assess quantity by qPCR. Paired-end 2×150-bp sequencing was performed on a NovaSeq 6000 instrument (Illumina, San Diego, CA, USA).…”

Section: Metagenomic Sequencing Of Intestinal Microbiotamentioning

confidence: 99%

A multi‐omics analysis for the prediction of neurocognitive disorders risk among the elderly in Macao

Han

Quan

Chuang

et al. 2022

Clinical & Translational Med

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Background Due to the increasing ageing population, neurocognitive disorders (NCDs) have been a global public health issue, and its prevention and early diagnosis are crucial. Our previous study demonstrated that there is a significant correlation between specific populations and NCDs, but the biological characteristics of the vulnerable group predispose to NCDs are unclear. The purpose of this study is to investigate the predictors for the vulnerable group by a multi‐omics analysis. Methods Multi‐omics approaches, including metagenomics, metabolomic and proteomic, were used to detect gut microbiota, faecal metabolites and urine exosome of 8 normal controls and 13 vulnerable elders after a rigorous screening of 400 elders in Macao. The multi‐omics data were analysed using R and Bioconductor. The two‐sided Wilcoxon's rank‐sum test, Kruskal–Wallis rank sum test and the linear discriminant analysis effective size were applied to investigate characterized features. Moreover, a 2‐year follow‐up was conducted to evaluate cognitive function change of the elderly. Results Compared with the control elders, the metagenomics of gut microbiota showed that Ruminococcus gnavus , Lachnospira eligens , Escherichia coli and Desulfovibrio piger were increased significantly in the vulnerable group. Carboxylates, like alpha‐ketoglutaric acid and d ‐saccharic acid, and levels of vitamins had obvious differences in the faecal metabolites. There was a distinct decrease in the expression of eukaryotic translation initiation factor 2 subunit 1 (eIF2α) and amine oxidase A (MAO‐A) according to the proteomic results of the urine exosomes. Moreover, the compound annual growth rate of neurocognitive scores was notably decreased in vulnerable elders. Conclusions The multi‐omics characteristics of disturbed glyoxylate and dicarboxylate metabolism (bacteria), vitamin digestion and absorption and tricarboxylic acid cycle in vulnerable elders can serve as predictors of NCDs risk among the elderly of Macao. Intervention with them may be effective therapeutic approaches for NCDs, and the underlying mechanisms merit further exploration.

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Characterization of conjunctival microbiome dysbiosis associated with allergic conjunctivitis

Cited by 2 publications

References 49 publications

Epithelial barrier dysfunction in ocular allergy

Epithelial barrier dysfunction in ocular allergy

A multi‐omics analysis for the prediction of neurocognitive disorders risk among the elderly in Macao

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