Characterization of Complete Genome Sequences of a Porcine Endogenous Retrovirus Isolated From China Bama Minipig Reveals an Evolutionary Time Earlier Than That of Isolates From European Minipigs

Selective human cells in vitro transduction with porcine endogenous retrovirus (PERV)

“…Thus, the coefficient of variation determined at the amino acid sequence level (including only non-synonymous polymorphisms) was about 1.3% for the complete sequence of the pol gene (1147 aa), 2.3% for the gag gene (fragment of 87 aa) and 0.8% for the envA gene (fragment of 121 aa). The above results were consistent with the literature data, in particular with analysis of the entire genome of PERV-A-BM isolated from the Chinese breed of pigs, Guangxi Bam (Tang et al, 2016). Within the 8,774-nucleotide-long PERV-A-BM, the authors revealed three substitutions in gag and env genes (each causing a change in the amino acid sequence).…”

Porcine Endogenous Retrovirus (PERV) – Molecular Structure and Replication Strategy in the Context of Retroviral Infection Risk of Human Cells

“…Porcine endogenous retrovirus, as an ERV, occurs mainly in the form of provirus integrated within the DNA of the host (pig). The length of the provirus is about 9000 bp ( Czauderna et al, 2000 ; Krach et al, 2001 ; Preuss et al, 2006 ; Ma et al, 2010a ; Tang et al, 2016 ). Just like the virus genome, the provirus contains coding sequences gag, pol , and env .…”

“…PERVs are closely related to MLV, feline leukemia virus (FeLV), gibbon ape leukemia virus (GaLV), and koala retrovirus (KoRV) ( Denner, 2008 ). The estimated age of PERVs is about 7.4–8.3 million years ( Tonjes and Niebert, 2003 ; Niebert and Tonjes, 2005 ; Tang et al, 2016 ). Retroviruses are a virus family with single-stranded RNA (ssRNA) genomes characterized by the presence of reverse transcriptase (RT).…”