Characterization of combined cross-linked enzyme aggregates from laccase, versatile peroxidase and glucose oxidase, and their utilization for the elimination of pharmaceuticals

Touahar, Imad E.; Haroune, Lounès; Ba, Sidy; Bellenger, Jean-Phillipe; Cabana, Hubert

doi:10.1016/j.scitotenv.2014.01.132

Cited by 133 publications

(76 citation statements)

References 61 publications

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“…C 6 and C24 are control samples with no enzymes incubated for respectively 6 and 24 h, LP6 and LP24 are samples enzymatically pretreated for respectively 6 and 24 h. The data shows the increase of the parameters, as the measurements of the substrates at the start of the incubation are subtracted from the original data (n = 3). of a combination of laccase and versatile peroxidase was also reported in other studies, where a significant removal of pharmaceuticals was already obtained after 5 h (Touahar et al, 2014). The use of enzymes gives a significant difference in VSS (p = 0.041) of the solid fraction, total phenolic content (p < 0.001) and COD (p < 0.001) in the liquid fraction.…”

Section: Resultssupporting

confidence: 78%

Effect of enzymatic pretreatment of various lignocellulosic substrates on production of phenolic compounds and biomethane potential

Schroyen

Vervaeren

Vandepitte

et al. 2015

Bioresource Technology

103

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Section: Resultssupporting

confidence: 78%

Effect of enzymatic pretreatment of various lignocellulosic substrates on production of phenolic compounds and biomethane potential

Schroyen

Vervaeren

Vandepitte

et al. 2015

Bioresource Technology

103

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“…Meanwhile, the mechanisms by which SOD-2 can lead to increased cell death have been reported [35]. Also another mechanism for SOD-2 induced apoptosis is through its ability to activate p53 by production of H 2 O 2 [36].…”

Section: Discussionmentioning

confidence: 99%

Bisphenol A Induces Apoptosis in Liver Cells through Induction of ROS

Kourouma

Quan

Duan

et al. 2015

Advances in Toxicology

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Oxidative stress mechanisms are involved in hepatotoxicity. The liver is reported to be affected by bisphenol A (BPA) in animals studies and has been also reported to possess hepatic toxicity. This study aimed to examine association between liver health status and the effects of BPA on the antioxidant defense systems and liver biomarkers. BPA (0, 2, 10, and 50 mg/kg) body weight was mixed in corn oil and intraperitoneally administered every forty-eight hours for 30 days in dose-dependent manner. There was no significant difference between the body weight and weight of rat liver in BPA-treated groups and control groups. The study results show that the levels of malondialdehyde (MDA) and hydrogen peroxide (H 2 O 2 ) increased after exposure to BPA. However, the antioxidant enzymes catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GSH-PX) were significantly ( < 0.001, < 0.05, and < 0.001, resp.) decreased at 50 mg/kg dosage. Liver markers activities such as lactate dehydrogenase (LDH), glutamic-oxalacetic transaminase (GOT), and glutamic-pyruvic transaminase (GPT) were significantly increased, while -glutamyl transferase ( -GT) activity was decreased. BPA exposure increased activity of liver biomarkers indicating liver hyperactivity. Analysis of the liver section provided essential evidence of liver apoptosis. Moreover, BPA may lead to induced toxic response of liver oxidative system.

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“…In another study, a wide range of pharmaceutically active compounds were transformed by three cross-linked oxidative enzymes: laccase from Trametes versicolor, VP from B. adusta, and glucose oxidase from Aspergillus niger. These cross-linked aggregates lead to the formation of insoluble and versatile biocatalysts with expanded substrate ranges as well as versatile operating conditions (Touahar et al 2014). Pleurotus MnPs and VPs were also studied for use in specific applications, mainly for decolorization of textile dyes (Heinfling et al 1998;Zhao et al 2007;Solís et al 2012).…”

Section: Biotechnological Applicationsmentioning

confidence: 99%

The ligninolytic peroxidases in the genus Pleurotus: divergence in activities, expression, and potential applications

Knop

Yarden

Hadar

2014

Appl Microbiol Biotechnol

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Mushrooms of the genus Pleurotus are comprised of cultivated edible ligninolytic fungi with medicinal properties and a wide array of biotechnological and environmental applications. Like other white-rot fungi (WRF), they are able to grow on a variety of lignocellulosic biomass substrates and degrade both natural and anthropogenic aromatic compounds. This is due to the presence of the non-specific oxidative enzymatic systems, which are mainly consisted of lacasses, versatile peroxidases (VPs), and short manganese peroxidases (short-MnPs). Additional, less studied, peroxidase are dye-decolorizing peroxidases (DyPs) and heme-thiolate peroxidases (HTPs). During the past two decades, substantial information has accumulated concerning the biochemistry, structure and function of the Pleurotus ligninolytic peroxidases, which are considered to play a key role in many biodegradation processes. The production of these enzymes is dependent on growth media composition, pH, and temperature as well as the growth phase of the fungus. Mn(2+) concentration differentially affects the expression of the different genes. It also severs as a preferred substrate for these preoxidases. Recently, sequencing of the Pleurotus ostreatus genome was completed, and a comprehensive picture of the ligninolytic peroxidase gene family, consisting of three VPs and six short-MnPs, has been established. Similar enzymes were also discovered and studied in other Pleurotus species. In addition, progress has been made in the development of molecular tools for targeted gene replacement, RNAi-based gene silencing and overexpression of genes of interest. These advances increase the fundamental understanding of the ligninolytic system and provide the opportunity for harnessing the unique attributes of these WRF for applied purposes.

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Characterization of combined cross-linked enzyme aggregates from laccase, versatile peroxidase and glucose oxidase, and their utilization for the elimination of pharmaceuticals

Cited by 133 publications

References 61 publications

Effect of enzymatic pretreatment of various lignocellulosic substrates on production of phenolic compounds and biomethane potential

Effect of enzymatic pretreatment of various lignocellulosic substrates on production of phenolic compounds and biomethane potential

Bisphenol A Induces Apoptosis in Liver Cells through Induction of ROS

The ligninolytic peroxidases in the genus Pleurotus: divergence in activities, expression, and potential applications

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