Characterization of colonic dendritic cells in normal and colitic mice

Cruickshank, Sheena M.; English, Nicholas R.; Felsburg, Peter J.; Carding, Simon R.

doi:10.3748/wjg.v11.i40.6338

Cited by 37 publications

(37 citation statements)

References 32 publications

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“…In accordance with these reports, we too found a sizable fraction of colonic DCs to be CD103 ϩ . The phenotypic characteristics of colonic DCs described herein are similar to what was reported from studies of colonic cells isolated from naive mice by gating on CD11c ϩ MHC class II ϩ cells during flow cytometric analysis (17) and detected by immunofluorescence analysis (37). Thus, isolation of colonic DCs using MACS does not seem to cause phenotypic alteration, and this method appears to allow for isolation of different DC populations without biasing one subset over another.…”

Section: Discussionsupporting

confidence: 66%

Dendritic Cells Derived from Murine Colonic Mucosa Have Unique Functional and Phenotypic Characteristics

Takenaka

Safroneeva

Xing

et al. 2007

The Journal of Immunology

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Dendritic cells (DCs) residing in different tissues and exposed to different organisms are likely to have different reactivities to their surrounding environment. Many studies use in vitro generated DCs to examine functions of these cells, but such cells may not truly reflect the nature of DCs and their in situ activities in vivo. We have used magnetic label-based technique to isolate colonic DCs to conduct derailed characterization of these cells. Colonic DCs comprise mainly CD11b+ DCs with few CD8α+ DCs or plasmacytoid DCs. Functionally, isolated colonic DCs are able to endocytose and process proteins, undergo maturation, and stimulate T cells to proliferate. Importantly, expression of TLRs by colonic DCs is significantly lower than that of their spleen counterparts; however, they appear to be as, or more, responsive to stimulation by oligodeoxynucleotides containing CpG motif based on their cytokine production. We speculate that colonic DCs have unique reactivities differing from DCs residing in other lymphoid tissues and are adapted for the unique microenvironment of the colonic mucosa and that these cells react uniquely to their environment.

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Section: Discussionsupporting

confidence: 66%

Dendritic Cells Derived from Murine Colonic Mucosa Have Unique Functional and Phenotypic Characteristics

Takenaka

Safroneeva

Xing

et al. 2007

The Journal of Immunology

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“…Additionally, two major subtypes of CD11c ϩ DC have been described, plasmacytoid and myeloid DC. Myeloid DC are CD11c ϩ , CD11b ϩ , CD8␣ Ϫ , and B220 Ϫ (2,3,19,80), whereas plasmacytoid DC are CD8␣ ϩ and B220 ϩ and lack expression of costimulatory molecules (2,3,7,13,61,80). In preliminary studies with both naïve and infected mice, less than 3% of lung DC expressed B220 (Wozniak and Levitz, unpublished observations), suggesting that the vast majority of these DC are myeloid DC.…”

Section: Fig 4 Effect Of Infection With C Neoformans On the Percenmentioning

confidence: 99%

In Vivo Role of Dendritic Cells in a Murine Model of Pulmonary Cryptococcosis

2006

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Dendritic cells (DC) have been shown to phagocytose and kill Cryptococcus neoformans in vitro and are believed to be important for inducing protective immunity against this organism. Exposure to C. neoformans occurs mainly by inhalation, and in this study we examined the in vivo interactions of C. neoformans with DC in the lung. Fluorescently labeled live C. neoformans and heat-killed C. neoformans were administered intranasally to C57BL/6 mice. At specific times postinoculation, mice were sacrificed, and lungs were removed. Single-cell suspensions of lung cells were prepared, stained, and analyzed by microscopy and flow cytometry. Within 2 h postinoculation, fluorescently labeled C. neoformans had been internalized by DC, macrophages, and neutrophils in the mouse lung. Additionally, lung DC from mice infected for 7 days showed increased expression of the maturation markers CD80, CD86, and major histocompatibility complex class II. Finally, ex vivo incubation of lung DC from infected mice with Cryptococcus-specific T cells resulted in increased interleukin-2 production compared to the production by DC from naïve mice, suggesting that there was antigenspecific T-cell activation. This study demonstrated that DC in the lung are capable of phagocytosing Cryptococcus in vivo and presenting antigen to C. neoformans-specific T cells ex vivo, suggesting that these cells have roles in innate and adaptive pulmonary defenses against cryptococcosis.

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“…Separate studies suggest that CD11c ϩ CD8␣ ϩint CD11b ϩ cells could also be considered plasmacytoid DC (33). MLN CD11c ϩ CD8␣-DCs can be either nonplasmacytoid or myeloid DCs (36). mPDCA is expressed on peripheral plasmacytoid DCs (38); its expression on GALT DCs had not been described previously.…”

Section: Populations In the Mlns Of Tlr9 ϫ/ϫ Mice Are Reduced Compmentioning

confidence: 99%

TLR9 Is Required for the Gut-Associated Lymphoid Tissue Response following Oral Infection of Toxoplasma gondii

Minns

Ménard

Foureau

et al. 2006

The Journal of Immunology

120

111

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TLRs expressed by a variety of cells, including epithelial cells, B cells, and dendritic cells, are important initiators of the immune response following stimulation with various microbial products. Several of the TLRs require the adaptor protein, MyD88, which is an important mediator for the immune response following Toxoplasma gondii infection. Previously, TLR9-mediated innate immune responses were predominantly associated with ligation of unmethylated bacterial CpG DNA. In this study, we show that TLR9 is required for the Th1-type inflammatory response that ensues following oral infection with T. gondii. After oral infection with T. gondii, susceptible wild-type (WT; C57BL/6) but not TLR9−/− (B6 background) mice develop a Th1-dependent acute lethal ileitis; TLR9−/− mice have higher parasite burdens than control WT mice, consistent with depressed IFN-γ-dependent parasite killing. A reduction in the total T cell and IFN-γ-producing T cell frequencies was observed in the lamina propria of the TLR9−/− parasite-infected mice. TLR9 and type I IFN production was observed by cells from infected intestines in WT mice. TLR9 expression by dendritic cell populations is essential for their expansion in the mesenteric lymph nodes of infected mice. Infection of chimeric mice deleted of TLR9 in either the hemopoietic or nonhemopoietic compartments demonstrated that TLR9 expression by cells from both compartments is important for efficient T cell responses to oral infection. These observations demonstrate that TLR9 mediates the innate response to oral parasite infection and is involved in the development of an effective Th1-type immune response.

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Characterization of colonic dendritic cells in normal and colitic mice

Abstract: These findings demonstrate striking changes in the number, distribution and phenotype of DC in the inflamed colon. Their intimate association with lymphocytes in the colon and draining lymph nodes suggest that they may contribute directly to the ongoing inflammation in the colon.

Cited by 37 publications

References 32 publications

Dendritic Cells Derived from Murine Colonic Mucosa Have Unique Functional and Phenotypic Characteristics

Dendritic Cells Derived from Murine Colonic Mucosa Have Unique Functional and Phenotypic Characteristics

In Vivo Role of Dendritic Cells in a Murine Model of Pulmonary Cryptococcosis

TLR9 Is Required for the Gut-Associated Lymphoid Tissue Response following Oral Infection of Toxoplasma gondii

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