Characterization of Colletotrichum isolates causing avocado anthracnose and first report of C. gigasporum infecting avocado in Sri Lanka

Hunupolagama, D. M.; Wijesundera, R.L.C.; Chandrasekharan, N.V.; Wijesundera, W.S.S.; Kathriarachchi, Hashendra; Fernando, T.H.P.S.

doi:10.5943/ppq/5/2/10

Cited by 13 publications

(8 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…TUB2 sequences, generated for C. endophytica in the present study and deposited in the GenBank, would therefore be a valuable source of reference sequence material for future studies of Colletotrichum. The present study did not encounter either C. gloeosporioides or C. gigasporum that were previously identified to be associated with the avocado anthracnose in Sri Lanka (Hunupolagama et al, 2015).…”

Section: Discussioncontrasting

confidence: 51%

Molecular phylogeny-based identification of Colletotrichum endophytica and C. siamense as causal agents of avocado anthracnose in Sri Lanka

et al. 2021

View full text Add to dashboard Cite

Avocado (Persea americana) is a sub-tropical fruit with high nutritional value and numerous health benefits. Among the postharvest fungal diseases that affect ripe avocados, anthracnose is one of the most destructive disease worldwide, causing significant postharvest fruit losses and limiting shelf life. Over 15 Colletotrichum species have been reported as causing avocado anthracnose from avocado growing countries in the world. In the present study, 35 Colletotrichum isolates were obtained from ripe avocados showing anthracnose symptoms, collected from the Central and North Western Province of Sri Lanka. Fifteen randomly selected isolates were subjected to DNA sequence analysis using ITS, TUB2, and GAPDH regions. Species affiliations and identities of the resulting sequences were determined through similarity-based searches of the NCBI GenBank Database. Based on the combined phylogentic analysis of three gene regions, nine and six isolates were identified as C. endophytica and C. siamense respectively, both belonging to the C. gloeosporioides species complex. Of the two species, C. endophytica is reported as a causal agent of avocado anthracnose for the first time.

show abstract

Section: Discussioncontrasting

confidence: 51%

Molecular phylogeny-based identification of Colletotrichum endophytica and C. siamense as causal agents of avocado anthracnose in Sri Lanka

et al. 2021

View full text Add to dashboard Cite

show abstract

“…However, based on molecular analysis, C. gloeosporioides was also reported as the causative agent of avocado fruit anthracnose in Mexico [85], Mersin Province, Turkey [88], and Ghana [90]. Hunupolagama et al (2015) [86] identified C. gigasporum as a causative agent of avocado anthracnose in Sri Lanka based on four markers, ITS, actin (ACT), GAPDH, and β-tubulin. In Mexico, two species C. godetiae [87] and C. karstii [139] were identified as anthracnose pathogens on avocado.…”

Section: Avocado Anthracnosementioning

confidence: 99%

Diversity of Colletotrichum Species Associated with Anthracnose Disease in Tropical Fruit Crops—A Review

Zakaria

2021

Agriculture

View full text Add to dashboard Cite

In tropical fruit crops, anthracnose is mainly caused by species belonging to the fungal genus, Colletotrichum. These phytopathogens can infect several parts of the fruit crops; however, infection during postharvest or ripening stages is responsible for major economic losses. Due to the formation of black to dark brown sunken lesions on the fruit surface, anthracnose reduces fruit quality and marketability. Among the most common tropical fruit crops susceptible to anthracnose are mango, papaya, banana, avocado, guava, and dragon fruit; these are economically relevant products in many developing countries. It is important to document that the newly recorded Colletotrichum spp. associated with fruit anthracnose can infect multiple hosts, but some species may be host-specific. By using multiple markers, many phylogenetic species of Colletotrichum have been reported as anthracnose-causing pathogens. Taking into account that disease management strategies strongly rely on adequate knowledge of the causative agents, updated information on Colletotrichum species and the hazard posed by the most recently identified species in tropical fruit plantations and harvested fruits becomes vital. Besides, the newly recorded species may be important for biosecurity and should be listed as quarantine pathogens, considering that tropical fruits are traded worldwide.

show abstract

“…Different studies suggest a predominance of species belonging to the gloeosporioides species complex, followed by members of the acutatum and boninense complexes [ 13 , 14 , 15 , 17 ]. Similarly, C. gigasporum and an undescribed species of the magnum complex represent unique detections in Sri Lanka and Mexico, respectively [ 12 , 15 ]. In this study, we report for the first time the occurrence of a member of the dematium complex causing the disease in Chile.…”

Section: Discussionmentioning

confidence: 99%

“…The causal agents of avocado anthracnose are fungi of the genus Colletotrichum [ 11 ], and approximately 20 species have been reported worldwide, comprising members of five different species complexes, namely gloeosporioides, acutatum, boninense, gigasporum, and magnum [ 11 , 12 , 13 , 14 , 15 , 16 , 17 ]. In Chile, however, only C. gloeosporioides sensu lato has been reported [ 18 ], which produces orange-colored sporulating lesions.…”

Section: Introductionmentioning

confidence: 99%

First Record of Colletotrichum anthrisci Causing Anthracnose on Avocado Fruits in Chile

et al. 2022

View full text Add to dashboard Cite

Anthracnose caused by Colletotrichum species is one of the most frequent and damaging fungal diseases affecting avocado fruits (Persea americana Mill.) worldwide. In Chile, the disease incidence has increased over the last decades due to the establishment of commercial groves in more humid areas. Since 2018, unusual symptoms of anthracnose have been observed on Hass avocado fruits, with lesions developing a white to gray sporulation. Morphological features and multi-locus phylogenetic analyses using six DNA barcodes (act, chs-1, gapdh, his3, ITS, and tub2) allowed the identification of the causal agent as Colletotrichum anthrisci, a member of the dematium species complex. Pathogenicity was confirmed by inoculating healthy Hass avocado fruits with representative isolates, reproducing the same symptoms initially observed, and successfully reisolating the same isolates from the margin of the necrotic pulp. Previously, several Colletotrichum species belonging to other species complexes have been associated with avocado anthracnose in other countries. To our knowledge, this is the first record of C. anthrisci and of a species of the dematium species complex causing anthracnose on avocado fruits in Chile and worldwide.

show abstract

Characterization of Colletotrichum isolates causing avocado anthracnose and first report of C. gigasporum infecting avocado in Sri Lanka

Cited by 13 publications

References 30 publications

Molecular phylogeny-based identification of <em>Colletotrichum endophytica</em> and <em>C. siamense</em> as causal agents of avocado anthracnose in Sri Lanka

Molecular phylogeny-based identification of <em>Colletotrichum endophytica</em> and <em>C. siamense</em> as causal agents of avocado anthracnose in Sri Lanka

Diversity of Colletotrichum Species Associated with Anthracnose Disease in Tropical Fruit Crops—A Review

First Record of Colletotrichum anthrisci Causing Anthracnose on Avocado Fruits in Chile

Contact Info

Product

Resources

About